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Application of nitrogen and boron isotopes for tracing sources of anthropogenic contamination in Monforte-Alter do Chão aquifer system, Portugal

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Abstract

The Monforte-Alter do Chão aquifer system represents one of the main water resources in Alentejo region (SW Portugal), and is located in an area of intensive agriculture and cattle breeding. The groundwater shows varied chemical composition, reflecting fast chemical changes associated with the water–rock interaction, with waters of Ca–HCO3, Na/Ca–HCO3, and Na–HCO3 facies. Some of the waters have strong evidence of nitrogen contamination with nitrate concentrations above 50 mg/l. The implementation of the European Nitrates Directive and the Water Framework Directive has led to the designation of large areas as Nitrate Vulnerable Zones, for being at risk from agricultural nitrate pollution: groundwater containing or that could contain more than 50 mg/l of nitrates. Corrective measures taken to reverse the trend can only be established when the contaminant source is known. In this study, the isotopic compositions of NO3 (δ15N and δ18O) and B (δ11B) were used to identify the sources of the nitrogen contamination. Despite the occurrence of some evaporation processes, the δ15N and δ18O isotopes allowed to distinguish two groups of waters with different sources of nitrates, fertilizers, and soils or manure and domestic effluents. The analysis of the NO3 source based on δ11B isotope ratio pointed out contamination by pig farms effluents. However, considering the land occupation and the strong livestock activities in the area, it is possible that the adsorption of 11B isotopes onto clays or the high pH of the groundwater could explain the high δ11B values.

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(data from IPMA 2018)

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(adapted from Fernandes and Francés 2009)

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References

  • Almeida C, Mendonça JL, Jesus MR, Gomes AJ (2000) Sistemas Aquíferos de Portugal Continental, Relatório. INAG, Instituto da Água, Lisboa

    Google Scholar 

  • Araújo A (1995) Estrutura de uma geotransversal entre Brinches e Mourão (Zona de Ossa-Morena): implicações na evolução geodinâmica da margem sudoeste do Terreno Autóctone Ibérico.Tese de Doutoramento, Dep. Geociências da Univ. de Évora

  • Aravena R, Evans ML, Cherry JA (1993) Stable isotopes of oxygen and nitrogen in source identification of nitrate from septic systems. Ground Water 31:180–186

    Article  Google Scholar 

  • Aravena R, Mayer B, Aelion CM (2010) Isotopes and processes in the nitrogen and sulfur cycles. In: Höhener P, Hunkeler D, Aravena R (Eds.) Environmental isotopes in biodegradation and bioremediation. CRC Press, Boca Raton, pp 203–246

    Google Scholar 

  • Baily A, Rock L, Watson CJ, Fenton O (2011) Spatial and temporal variations in groundwater nitrate at an intensive dairy farm in south-east Ireland: insights from stable isotope data. Agric Ecosys Environ 144:308–318

    Article  Google Scholar 

  • Basset RL, Buszka PM, Davidson GR, Chong-Diaz D (1995) Identification of groundwater solute sources using boron isotopic composition. Environ Sci Technol 29:2915–2922

    Article  Google Scholar 

  • Bassett RL (1990) A critical evaluation of the available measurements for the stable isotopes of boron. Appl Geochem 5:541–554

    Article  Google Scholar 

  • Bohlke JK (2002) Groundwater recharge and agricultural contamination. Hydrogeol J 10:153–179

    Article  Google Scholar 

  • Bottcher J, Strebel O, Voerkelius S, Schmidt HL (1990) Using isotope fractionation of nitrate-nitrogen and nitrate-oxygen for evaluation of microbial denitrification in a sandy aquifer. J Hydrol 114:413–424

    Article  Google Scholar 

  • Carvalho MR, Fernandes P, Silva C (2011) Directiva Nitratos—Determinação da origem do nitrato no sistema aquífero de Monforte-Alter do Chão utilizando técnicas isotópicas. Anexo. INAG e FFCUL, Lisboa

    Google Scholar 

  • Clark I, Fritz P (1997) Environmental isotopes in hydrogeology. Lewis Publishers, Boca Raton

    Google Scholar 

  • Costa A (1995) Avaliação de Recursos Hídricos Subterrâneos na Herdade de Torre de Palma. Relatório Interno, IGM, Lisboa

    Google Scholar 

  • Costa IR, Barriga F, Mata J, Munhá JM (1993) Rodingitization and serpentinization processes in Alter do Chão Massif (NE Alentejo). In: Noronha F, Marques M, Nogueira P (eds) Actas da IX Semana de Geoquímica. Universidade do Porto. Faculdade de Ciências. Museu e Laboratório Mineralógico e Geológico, Porto, pp 27–31

    Google Scholar 

  • Craig H (1961) Standard for reporting concentrations of deuterium and oxygen-18 in natural water. Science 133:1833–1834

    Article  Google Scholar 

  • Craig H, Gordon LI, Horibe Y (1963) Isotopic exchange effects in the evaporation of water. J Geophys Res 68:5079–5087

    Article  Google Scholar 

  • Curt MD, Aguado P, Sánchez G, Bigeriego M, Fernández J (2004) Nitrogen isotope ratios of synthetic and organic sources of nitrate water contamination in Spain. Water Air Soil Pollut 151:135–142

    Article  Google Scholar 

  • Delgado JFN (1904) Faune cambrienne du Haut-Alemtejo (Portugal) Comunicações da Comissão do Serviço. Geológico de Portugal 5:307–374

    Google Scholar 

  • Eisenhut S, Heumann KG, Vengosh A (1996) Determination of boron isotopic variations in aquatic systemswith negative thermal ionizationmass spectrometry as a tracer for anthropogenic influences. Fresenius J Anal Chem 354:903–909

    Google Scholar 

  • ERHSA (2001) Estudo dos recursos hídricos subterrâneos do Alentejo—Relatório Técnico. Universidade de Évora, Instituto da Água, Instituto Geológico e Mineiro, Direcção Regional do Ambiente do Alentejo, Beja

    Google Scholar 

  • European Commission (1991) Directive 91/676/EEC. Council Directive of 12 December 1991 concerning the protection of waters against pollution caused by nitrates from agricultural sources. Off J Eur Commun L375:1–8

    Google Scholar 

  • European Commission (2000) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Off J Eur Commun L327:1–72

    Google Scholar 

  • Faure G, Mensing T (2005) Isotopes: principles and applications. Wiley, New Jersey

    Google Scholar 

  • Fernandes J (2001) Sistema Aquífero Monforte-Alter do Chão. Fichas dos Sistemas Aquíferos do Alentejo—Anexo II do Relatório Técnico do ERHSA (IGM). Publicado pela Comissão de Coordenação da Região Alentejo, Évora

    Google Scholar 

  • Fernandes J, Francés A (2009) Sistema Aquífero Monforte-Alter do Chão: resultados obtidos no âmbito do Estudo dos Recursos Hídricos Subterrâneos do Alentejo, ERHSA. AHR Tejo, Beja

    Google Scholar 

  • Fernandes J, Ribeiro L (2001) A geomathematical approach to characterizing the spatio-temporal groundwater flow in Monforte-Alter do Chão aquifer, Portugal. In: Seiler K-P, Wohnlich S (eds) New approaches characterizing groundwater flow, vol 1. A.A. Balkema, Swets & Zeitlinger B.V., Lisse, pp 311–314

    Google Scholar 

  • Gonçalves F, Fernandes A (1997) Carta Geológica de Portugal na escala de 1:50000 e notícia explicativa da folha 32-B (Portalegre). Serviços Geológicos de Portugal, Lisboa

    Google Scholar 

  • Gonçalves F, Zbyszewski G, Coelho A (1975) Carta Geológica de Portugal na escala de 1:50000 e notícia explicativa da folha 32-D (Sousel). Serviços Geológicos de Portugal, Lisboa

    Google Scholar 

  • Guy R, Berry J, Fogel M, Hoering T (1989) Differential fractionation of oxygen isotopes by cyanide-resistant and cyanide-sensitive respiration in plants. Planta 177:483–491

    Article  Google Scholar 

  • Heaton THE (1986) Isotopic studies of nitrogen pollution in the hydrosphere and atmosphere: a review. Chem Geol 59:87–102

    Article  Google Scholar 

  • IAEA/WMO (2017) Global network of isotopes in precipitation. The GNIP Database. https://nucleus.iaea.org/wiser. Accessed 19 Feb 2018

  • IPMA (2018) Normais climatológicas 1981–2010 (provisórias) de Portalegre. https://www.ipma.pt/pt/oclima/normais.clima/1981-2010/015/. Accessed 15 Dec 2017

  • Kendall C (1998) Tracing nitrogen sources and cycles in catchments. In: Kendall C, McDonnell JJ (eds) Isotope tracers in catchment hydrology. Elsevier, Amsterdam, pp 519–576

    Chapter  Google Scholar 

  • Kendall C, McDonnell JJ (eds) (1998) Isotope tracers in catchment hydrology. Elsevier Science B.V., Amsterdam

    Google Scholar 

  • Kendall C, Elliott EM, Wankel SD (2007) Tracing anthropogenic inputs of nitrogen in ecosystems. In: Michener R, Lajtha K (eds) Stable isotopes in ecology and environmental science, 2nd edn. Blackwell Publishing Inc., Oxford, pp 375–449

    Chapter  Google Scholar 

  • Komor SC (1997) Boron contents and isotopic compositions of hog manure, selected fertilizers, and water in Minnesota. J Environ Qual 26:1212–1222

    Article  Google Scholar 

  • Kreitler CW (1979) Nitrogen isotope ratio studies of soils and groundwater nitrate from alluvial fan aquifer in Texas. J Hydrol 42:147–170

    Article  Google Scholar 

  • Kristensen E (1990) Characterization of biogenic organic matter by stepwise thermogravimetry (STG). Biogeochemistry 9:135–159

    Article  Google Scholar 

  • Lane GA, Dole M (1956) Fractionation of oxygen isotopes during respiration. Science 123:574–576

    Article  Google Scholar 

  • Lopes JC, Munhá JM, Wu CT, Oliveira VMJ (1998) O Complexo Plutónico de Monforte-Santa Eulália (Alentejo-NE, Portugal central): caracterização geoquímica e considerações petrogenéticas. Comun Inst Geol e Mineiro 83:127–142

    Google Scholar 

  • Marques JM, Carreira PM, Carvalho MR, Matias MJ, Goff FE, Basto MJ, Graça RC, Aires-Barros L, Rocha L (2008) Origins of high pH mineral waters from ultramafic rocks, Central Portugal. Appl Geochem 23(12):3278–3289

    Article  Google Scholar 

  • Michalski G, Kolanowski M, Rihaa KM (2015) Oxygen and nitrogen isotopic composition of nitrate in commercial fertilizers, nitric acid, and reagent salts. Isot Environ Health Stud 51:382–391

    Article  Google Scholar 

  • Novotny V, Olem H (1994) Water Quality: Prevention, Identification, and Management of Diffuse Pollution. Wiley, New Jersey

    Google Scholar 

  • Panno SV, Hackley KC, Hwang HH, Kelly WR (2001) Determination of the sources of nitrate contamination in karst springs using isotopic and chemical indicators. Chem Geol 179:113–128

    Article  Google Scholar 

  • Puig R, Soler A, Widory D, Mas-Pla J, Domènecha C, Otero N (2017) Characterizing sources and natural attenuation of nitrate contamination in the Baix Ter aquifer system (NE Spain) using a multi-isotope approach. Sci Total Environ 580:518–532

    Article  Google Scholar 

  • Raposo J, Nunes A (1978) Balanço Hídrico do Continente. Comissão Nacional do Ambiente, Lisboa

    Google Scholar 

  • Rebelo A (2016) Origem de Nitratos na Massa de Água Monforte-Alter do Chão: Contribuição dos Solos. Projeto de Investigação, Mestrado em Geologia Aplicada, Especialização em Hidrogeologia. Faculd. Ciências. Universidade Lisboa, Lisboa

    Google Scholar 

  • Sacchi E (2013) Origin and fate of nitrates in groundwater from the central Po Plain: insights from isotopic investigations. Appl Geochem 34:164–180

    Article  Google Scholar 

  • Tirez K, Brusten W, Widory D, Petelet E, Bregnot A, Xue D, Boeckx P, Bronders J (2010) Boron isotope ratio (δ11B) measurements in Water Framework Directive monitoring programs: comparison between double focusing sector field ICP and thermal ionization mass spectrometry. J Anal Atom Spectrom 25:964–974

    Article  Google Scholar 

  • Vane CH, Kim AW, McGowan S, Leng MJ, Heaton THE, Kendrick CP, Coombs P, Yang H, Swann GEA (2010) Sedimentary records of sewage pollution using faecal markers in contrasting peri-urban shallow lakes. Sci Total Environ 409:345–356

    Article  Google Scholar 

  • Vengosh A (1998) Boron isotopes and groundwater pollution. Water Environ News 3:15–16

    Google Scholar 

  • Vengosh A, Heumann KG, Juraske S, Kasher R (1994) Boron isotope application for tracing sources of contamination in groundwater. Environ Sci Technol 28:1968–1974

    Article  Google Scholar 

  • Vengosh A, Barth S, Heumann KG, Eisenhut S (1999) Boron isotopic composition of freshwater lakes from Central Europe and possible contamination sources. Acta Hydrochim Hydrobiol 27:416–421

    Article  Google Scholar 

  • Vitòria L, Otero N, Soler A, Canals A (2004) Fertilizer characterization: isotopic data (N, S, O, C, and Sr). Environ Sci Technol 38:3254–3262

    Article  Google Scholar 

  • Widory D, Kloppmann W, Chery L, Bonnin J, Rochdi H, Guinamant JL (2004) Nitrate in groundwater, an isotopic multi-tracer approach. J Contam Hydrol 72:165–188

    Article  Google Scholar 

  • Widory D, Petelet-Giraud E, Negrel P, Ladouche B (2005) Tracking the sources of nitrate in groundwater using coupled nitrogen and boron isotopes: a synthesis. Environ Sci Technol 39:539–548

    Article  Google Scholar 

  • Xue D, Botte J, De Baets B, Accoe F, Nestler A, Taylor P, Van Cleemput O, Berglund M, Boeckx P (2009) Present limitations and future prospects of stable isotope methods for nitrate source identification in surface- and groundwater. Water Res 43:1159–1170

    Article  Google Scholar 

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Acknowledgements

The authors are grateful for the financial support of the national agency for environmental protection—Agência Portuguesa do Ambiente—APA, I.P., and Fundação Ciência e Tecnologia—FCT project reference UID/GEO/50019/2013—Instituto Dom Luiz. Authors acknowledge the fruitful revision and contribution of the two anonymous reviewers.

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Authors and Affiliations

  1. Departamento de Geologia, Faculdade de Ciências, Universidade de Lisboa, Ed. C6, Campo Grande, 1749-014, Lisbon, Portugal

    P. Fernandes, M. R. Carvalho, M. C. Silva, A. Rebelo & J. Zeferino

  2. Instituto D. Luiz, Faculdade de Ciências, Universidade de Lisboa, Ed. C1, Campo Grande, 1749-014, Lisbon, Portugal

    M. R. Carvalho

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  1. P. Fernandes
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  2. M. R. Carvalho
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  4. A. Rebelo
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  5. J. Zeferino
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Correspondence to M. R. Carvalho.

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This article is part of the special issue on Sustainable Resource Management: Water Practice Issues.

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Fernandes, P., Carvalho, M.R., Silva, M.C. et al. Application of nitrogen and boron isotopes for tracing sources of anthropogenic contamination in Monforte-Alter do Chão aquifer system, Portugal. Sustain. Water Resour. Manag. 5, 249–266 (2019). https://doi.org/10.1007/s40899-018-0265-1

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