Acta Theriologica

, Volume 57, Issue 3, pp 217–223

Patterns of variation in reproductive parameters in Eurasian lynx (Lynx lynx)

  • Erlend B. Nilsen
  • John D. C. Linnell
  • John Odden
  • Gustaf Samelius
  • Henrik Andrén
Open Access
Original Paper

DOI: 10.1007/s13364-011-0066-5

Cite this article as:
Nilsen, E.B., Linnell, J.D.C., Odden, J. et al. Acta Theriol (2012) 57: 217. doi:10.1007/s13364-011-0066-5

Abstract

Detailed knowledge of the variation in demographic rates is central for our ability to understand the evolution of life history strategies and population dynamics, and to plan for the conservation of endangered species. We studied variation in reproductive output of 61 radio-collared Eurasian lynx females in four Scandinavian study sites spanning a total of 223 lynx-years. Specifically, we examined how the breeding proportion and litter size varied among study areas and age classes (2-year-old vs. >2-year-old females). In general, the breeding proportion varied between age classes and study sites, whereas we did not detect such variation in litter size. The lack of differences in litter sizes among age classes is at odds with most findings in large mammals, and we argue that this is because the level of prenatal investment is relatively low in felids compared to their substantial levels of postnatal care.

Keywords

Demography Reproduction Carnivores Litter size Breeding proportion 

Introduction

Describing and quantifying factors that contribute to variation in vital rates are crucial to our understanding of the evolution of life histories, population dynamics, and to our ability to understand factors affecting population viability in conservation contexts (Kerley et al. 2003). In long-lived species with long generation times, such as large herbivores and large carnivores, the functional relationship between long-term population growth and adult survival is stronger than that for recruitment (Gaillard et al. 2000; Bielby et al. 2007; Nilsen et al. 2009). However, the impact of a vital rate on observed variation in population growth rate is the product of the elasticity of population growth rate and the coefficient of variation in that parameter as well as the actual variation in that parameter (Coulson et al. 2005; Horvitz et al. 1997). As variation usually reduces the long-term growth rate of populations (Tuljapurkar and Orzack 1980), reproductive output typically shows more pronounced variation across time and space than survival rates (Gaillard et al. 2000; Gaillard and Yoccoz 2003). Understanding sources of variation in reproductive output is therefore of crucial importance for successful conservation and management planning.

Variation in fertility rates is caused by variation in a sequence of factors that together define fertility. For example, if defining fertility as the number of offspring per female at birth (Caswell 2001), fertility is given by the product between the proportion of females that breed in a given year and the litter sizes of breeding females. In polytocous species (such as the Eurasian lynx, Lynx lynx, which is studied here) these rates might vary independently of each other. Both phylogenetic and physiological constraints, and adaptive allocation to current reproduction relative to survival and future fitness prospects (Stearns 1992) might induce variation in reproductive output.

In long-lived iteroparous species, reproductive output is often strongly age-dependent (Gaillard et al. 1992), with a lower reproductive output in first-time breeders (Forslund and Part 1995). Such patterns could arise due to several factors, both due to constraints in the skills of young animals (Forslund and Part 1995; Rutz et al. 2006) and due to restraints (Curio 1983); i.e., young animals might put less effort into breeding because reproductive effort might increase mortality and/or reduce future reproductive success (Stearns 1992). Furthermore, varying ecological conditions might cause fertility to vary also within age classes. In general, low food availability and high population densities have been shown to reduce fertility rates especially among young individuals (Gaillard et al. 1998b; Gaillard et al. 2000). The relationship between fertility rate and ecological conditions is often more pronounced in certain stages of the life cycle, and it is generally acknowledged that reproductive output among young females is among the first traits affected when ecological conditions get harsh (Gaillard et al. 2000). Finally, as late gestation and lactation are commonly regarded as costly, spontaneous or selective abortion (as well as perinatal survival) might result in differences in the proportion of pregnant females that give birth to viable young, despite no differences in the proportion that implant and become pregnant. If such early mortality/abortion is not independent among litter mates, this should create variation in breeding proportion rather than litter sizes.

Eurasian lynx have a wide distribution, covering much of the boreal forest in Eurasia (von Arx et al. 2004). The combined population from Norway and Sweden is >2,000 individuals implying a high degree of viability (Andrèn and Liberg 2008; Brøseth and Tovmo 2011). However, the species is associated with widespread conflicts (with livestock breeders and hunters) and is therefore subject to intensive hunting and lethal control (Nilsen et al., online early), as well as poaching (Andrèn et al. 2006; Linnell et al. 2010). Thus, lynx is a species of conservation concern subject to active management in Scandinavia and its conservation requires solid biological knowledge. This includes high-quality demographic data to be used when setting hunting quotas and when monitoring the population size based on surveys of lynx family groups (i.e., females with dependent kittens) (Linnell et al. 2010; Andrèn et al. 2002). To date, the only available published data on lynx reproductive rates are from a reintroduced population in the Jura Mts. (Breitenmoser-Würsten et al. 2007), captive lynx (Henriksen et al. 2005), and some older Norwegian data extracted from shot lynx (Kvam 1991). Based on an exceptionally large data base of radio-collared Eurasian lynx from four study sites in Scandinavia (n = 61 female lynx followed for a total of 223 lynx years) we examined how breeding proportion and litter size varied among age classes (2-year-old vs. >2-year-old females) and between lynx populations across a range of ecological settings. Based on available literature from large mammals (see above sections) we expected that (a) the proportion of young females producing offspring should be higher in the southern populations with higher prey densities, and (b) a lower proportion of young compared to older females should produce offspring. Furthermore, we expected that c) the litter sizes of prime aged females should be larger than those of young females.

Methods

Study areas

This study was based on individual data obtained from radio-collared lynx in four study areas in Scandinavia: Sarek and Bergslagen in Sweden, Hedmark and Akershus in Norway (Table 1; Fig. 1). The northernmost study area is partly located within the Sarek National Park around Kvikkjokk in the county of Norrbotten (67°00′N, 17°40′E) and consists of a mixture of coniferous forests, mountain birch Betula sp. forest and alpine tundra in mountainous terrain. The central area is situated in the county of Hedmark in southeastern Norway (61°15′N, 11°30′E). The topography of the area consists of several parallel river valleys running from north to south, and about 72% of the area consists of coniferous forest. The Akershus study area (59°45′N, 11°15′E) is situated around the Norwegian capital, Oslo, and is similar to the Hedmark area although the proportion of farmland and human density is much higher and the topography is less hilly. The Bergslagen study area is located around Grimsö wildlife research station (59°30′N, 15°30′E) in south-central Sweden. The area is dominated by coniferous forest, and the proportion of agricultural land is higher in the southern parts (about 20%) and decreases towards the northern parts (<1% of the area). Roe deer (Capreolus capreolus) are the main prey in the Hedmark, Akershus and Bergslagen study areas, while semi-domestic reindeer (Rangifer tarandus) are the main prey in the Sarek area where roe deer are absent (Andrèn et al. 2006; Odden et al. 2006). In addition, a range of smaller species such as capercaille (Tetrao urogallus), black grouse (T. tetrix), mountain hares (Lepus timidus) and red foxes (Vulpes vulpes) are available in all sites, together with domestic sheep in the Norwegian sites during summer (see Andrèn et al. 2002; Andrèn et al. 2006; Linnell et al. 2001).
Table 1

Basic description of the study sites

Study site

Sarek (Sweden)

Hedmark (Norway)

Akershus (Norway)

Bergslagen (Sweden)

Latitude

67°N

61° 30′N

59°46′N

59°30′N

Habitat

BF/LA/HA

BF

BF

BF

Wild preya

Ptarmigan, mountain hare, capercaille, black grouse

Roe deer,* red deer, wild reindeer, mountain hare, capercaille, black grouse

Roe deer,* mountain hare, capercaille, black grouse

Roe deer,* mountain hare, brown hare, capercaille, black grouse

Domestic prey

Semidomestic reindeer*

Sheep

Sheep

 

Density of main preyb

1–2/km2

0.3/km2

1–10/km2

3–4/km2

BF boreal forest, LA low alpine (above the treeline), HA high alpine

aThe main prey species in each study area is marked with an asterisk

bReferences for densities of main prey: Danell et al. (2006) for Sweden and Linnell et al. (2007) for Norway

Fig. 1

Map showing the location of the study sites across Scandinavia. a Sarek, b Hedmark, c Akershus, d Bergslagen

Field procedures

This project is part of the large scale research project Scandlynx (http://scandlynx.nina.no/) that coordinates research efforts on lynx in Norway and Sweden. Lynx were captured using several methods, including darting from helicopters, box-traps, foot-snares placed at fresh kills, and by using dogs to chase lynx into trees. The captured lynx were immobilized and equipped with a radio-collar (either VHF or GPS) or an implanted radio transmitter, usually with a mortality function. The handling protocol for lynx has been examined by both the Swedish and the Norwegian Experimental Animal Ethics Committee and fulfils their ethical requirements for research on wild animals. For further description of capture techniques, see Andrèn et al. (2002) and Arnemo et al. (1999, 2006).

The lynx were radio-tracked at least two to four times per month, but normally more often. In May and June, the reproductive status of all female lynx was examined by more frequent localisation of radio-marked females from the ground and or the air, and counting the kittens in the natal lair if movement data indicated that she had given birth (Schmidt 1998). Field protocols varied between study areas (the wilderness nature of the Sarek site imposed logistical challenges) and with time (as VHF telemetry gave way to GPS technology with UHF and GSM downloads). Potentially reproductive females were tracked as often as possible during the birth period in May and early June. In most areas, we radio-tracked female lynx several times per week during the birth period. This sampling protocol would have made it difficult to detect females who gave birth and subsequently lost their kittens in the first few days of life, so our estimate of reproductive rates refers to those that had kittens that lived to at least 2 weeks of age. However, because females adopt a central place foraging behavior centered on a natal lair for the first 6–8 weeks of the kittens lives it was almost impossible to have failed to detect a reproductive event where kittens survived the perinatal period. Once reproduction was established we visited the natal lair site to count, weight, sex and mark (usually with microchips and/or tattoos, but occasionally with intraperitoneal implants). Our visits were normally conducted when kittens were between 2 and 6 weeks of age. The number of reproductive events and female lynx included in the analysis in this study is reported in Table 2.
Table 2

Number of reproductive events and number of female lynx included in this study

 

2 years

>2 years

Lynx individuals

Years

Sarek

13

92

22

1994–2007

Hedmark

4

38

11

1996–2006

Akershus

4

14

6

2001–2006

Bergslagen

19

39

20

1997–2006

The age at capture was known for animals originally marked in their lairs, whereas we classed previously unmarked animals that were captured during winter as subadult (<18 months old) and adult (>18 months old) based on whether they were structurally fully grown. A large portion of animals were captured in spring when still travelling with their mothers and <1 year old, which further helped to determine the age of animals. In addition, age was also in many cases determined retrospectively post mortem by counting incremental lines in the tooth cementum (see Kvam 1984) when animals died or were shot.

Statistical analysis

We used generalized linear mixed effects models implemented with the lmer function in library lme4 (Bates et al. 2010) in software R 2.12.1 (R Development Core Team 2010) to examine patterns of variation in lynx breeding performance. Specifically, we examined how the breeding proportion and litter size of lynx varied among study sites and age classes. In the models examining patterns in breeding proportion, we used a binomial error term for the fixed components, whereas we used a Poisson error term when the focus was on litter sizes. We assessed models with the main effects of study area and age class, as well as their interaction, and selected the most parsimonious model in the set based on Akaike’s Information Criterion (AIC) (Burnham and Anderson 2002). To assess the relative support for each model, we also computed Akaike weights for each model in the set giving a measure of relative support for each model among the considered candidate models. To examine the patterns of age dependency in reproductive output, we used both a two age-class model contrasting young females (2 years of age) with older females (>2 years of age), and a full-age model including only females of known age and older than 2 years.

Results

There was substantial variation between study areas and age classes in the proportion of lynx females that gave birth (Tables 3 and 4), and the model that best described the variation in breeding proportion included the main effects of study area and age class (Table 3). In general, the proportion of 2-year-old lynx females that gave birth was lower than that of the older females (Table 3). Among females in the older age class (i.e., >2 years old) there was no indication for variation in the proportion of females that gave birth with increasing age in any study area or for all study areas combined ( ΔAIC = 1.53 for the model including age and ΔAIC = 0.86 for the model including a squared effect of age; the null model included only the intercept).
Table 3

Breeding proportion and litter size of lynx in the study sites included in this study, estimated with generalized linear mixed effects models with individual as a random factor (to account for pseudoreplication) and the two-way interaction between age class and study area as fixed effects

 

Breeding proportion

Litter size

2 years old

>2 years old

2 years old

>2 years old

Sarek

0.22 (0.06–0.53)

0.76 (0.62–0.87)

1.99 (1.30–3.04)

2.09 (1.90–2.30)

Hedmark

0.40 (0.09–0.81)

0.69 (0.53–0.81)

2.09 (1.13–3.87)

2.10 (1.83–2.41)

Akershus

0.50 (0.10–0.90)

0.81 (0.55–0.94)

1.90 (1.62–5.18)

1.95 (1.35–2.81)

Bergslagen

0.74 (0.49–0.89)

0.90 (0.76–0.96)

2.34 (1.94–2.81)

2.37 (2.10–2.69)

When analysing the breeding proportion a binomial error structure and logit link were used, whereas a Poisson error structure and log link were used to model litter sizes. Estimates are given as mean (95% CL)

Table 4

AIC values for models evaluating the variation in lynx breeding proportion and litter sizes in the study sites included in this study

 

Breeding proportion

Litter size

AIC

ΔAIC

wi

AIC

ΔAIC

wi

Intercept

292.70

16.70

0.000

45.70

0

0.644

Study area

291.50

15.5

0.000

50.52

4.82

0.058

Ageclass

284.10

8.1

0.016

47.40

1.70

0.275

Study area + Ageclass

276.00

0

0.890

52.48

6.78

0.022

Study area × Ageclassa

280.50

4.5

0.094

57.80

12.1

0.002

All models are generalized linear mixed effects models, with lynx identity fitted as random factor (intercept). The selected models (i.e., those with the lowest AIC values) are marked in bold

aModel includes main effects of study area and age class, as well as their interaction)

In general, litter size did not vary between study sites or age classes (Tables 3 and 4). Similarly, among females in the oldest age class (i.e., >2 years old), there was no indication for any difference in litter size (ΔAIC = 1.91 for the model including age; the null model included only the intercept).

Discussion

In this paper, we explored the patterns of spatial variation in reproductive rates in Eurasian lynx using a large sample of radio-collared lynx. We show that, in general, (1) litter size was less variable than the proportion of females that gave birth, (2) there were substantial differences between sites in the proportion of females that gave birth, and (3) that the variation in the breeding proportion was particularly pronounced among young females (2 years old).

The proportion of young females (i.e., 2 years old) that produced a litter varied markedly between our study populations. Such patterns in other species have often been linked to the prevailing ecological conditions (Gaillard et al.1992). For instance, it is generally acknowledged that age at maturity is one of the first life history traits to change when conditions switch from favorable to unfavorable (Eberhardt 1977, 2002). In general the breeding proportion was highest in the southernmost study population (Bergslagen), and lowest in the northernmost population (Sarek), with both the Norwegian populations showing intermediate values. Supporting these findings, we have previously found that the proportion of 1.5-year-old females that ovulate (i.e., that are sexually mature) was generally lower in the northernmost areas (Nilsen et al. 2010). Consequently, on a broad scale the patterns might indicate a slower life cycle in more extreme northern environments. Previous studies on lynx have indicated a clear effect of body mass on age at maturity (Nilsen et al. 2010). It is therefore possible that different maturation rates between sites will result in differential allocation to reproduction among yearling lynx in the different study areas (restraint hypothesis; Curio 1983), due to different growth rates affecting patterns of maturation in the populations. Although speculative, the different patterns in breeding proportions assessed by litter size observations and ovulation rates reported by Nilsen et al. (2010) suggest that the geographical variation in the breeding proportion may be caused by differences in feeding conditions and growth rates among areas. This theory is supported by the fact that body size and body mass of lynx in southern Sweden were related to roe deer availability during the first year of life (Yom-Tov et al. 2010). The lower reproductive rates in the Sarek study area may reflect their dependence on migratory semi-domestic reindeer as prey (Mattisson et al. online early; Pedersen et al. 1999), while the lynx are resident (Danell et al. 2006) and thus face a high degree of seasonality in their access to prey.

While there was a clear difference between females that were 2 years old and those that were older in the proportion that gave birth, litter sizes did not differ between these age classes, indicating that females that mature early invest relatively heavily in their first litter (see also Nilsen et al. 2010). This might be somewhat surprising given the potentially high costs of reproduction at low age in carnivores (Proaktor et al. 2007). In fact, the lack of age-dependency in litter sizes is unusual for polytocous species, where litter sizes are generally higher for multiparous than primiparous females (Ericsson et al. 2001; Forslund and Part 1995). Moreover, litter sizes were very similar across study sites, but our data did not allow us to investigate whether early mortality happened before or after parturition, as we only had measures of litter size from when kittens were about 1 month old (see Methods). The fact that litter sizes did not vary between study sites or age classes, might indicate that whenever early mortality occurred, the fate of litter mates were dependent on each other so that the mean litter size did not change. If they were, one would expect smaller litter sizes in areas with low breeding proportion. Such family effects in early survival have been documented in studies of other species and greatly increase the variation in reproductive success among individuals (Pettorelli and Durant 2007; Gaillard et al. 1998a; Panzacchi et al. 2009).

In summary, fertility is the product of the proportion of females that give birth and litter size. Different ecological and physiological process might cause variation in these traits. Specifically, our study suggests that variation in fecundity between sites and age classes was mostly caused by variation in the proportion of females that gave birth, whereas litter sizes were remarkably constant across sites and age classes. As expected from theory, between-site variation in breeding proportion was higher among young females than among older females. The reproductive parameters recorded in this study were very similar to those recorded from both wild lynx in the Jura Mts. (Breitenmoser-Würsten et al. 2007) and captive (Henriksen et al. 2005) populations of lynx from across European zoos, although the litter size decreased in the Jura Mts. population when there was a lack of mature males (Breitenmoser-Würsten et al. 2007). We doubt if this effect was present in our study sites. Although all our study areas were subject to lynx hunting and/or poaching in the period immediately prior to the mating season, the study areas are all imbedded in a large and continuous population with continuous habitat. This combined with the fact that harvest is usually evenly dispersed over large areas minimizes the chances of all males being removed from a given area and allows neighboring male lynx to expand their ranges to cover potentially receptive females. Furthermore, there are a large number of floating, non-territorial individuals who can rapidly occupy vacant territories (Samelius et al. 2011). The impression from these studies that span autochthonous, reintroduced, and captive populations is that lynx reproduction is remarkably constant, with the only substantial source of regional variation being caused by the proportion of 2-year-old females that breed.

Acknowledgements

The study was funded by the Swedish Environmental Protection Agency, the Norwegian Directorate for Nature Management, the Research Council of Norway, the European Union’s 7th Framework Programme, the World Wide Fund for Nature (Sweden), the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), the Swedish Association of Hunting and Wildlife Management, the private foundations “Olle och Signhild Engkvists Stiftelser”, “Marie- Claire Cronstedts Stiftelse”, and the Nature Protection Division of the County Governor’s Office for Oslo & Akershus, Østfold and, Hedmark. We are grateful to the many people who have assisted with field work through the years of this project’s life.

Open Access

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Copyright information

© The Author(s) 2012

Authors and Affiliations

  • Erlend B. Nilsen
    • 1
  • John D. C. Linnell
    • 1
  • John Odden
    • 1
  • Gustaf Samelius
    • 2
  • Henrik Andrén
    • 2
  1. 1.Norwegian Institute for Nature ResearchTrondheimNorway
  2. 2.Grimsö Wildlife Research Station, Department of EcologySwedish University of Agricultural SciencesRiddarhyttanSweden

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