Multipotent antioxidant and antitoxicant potentiality of an indigenous probiotic Bifidobacterium sp. MKK4

Abstract

Probiotic bacteria are now becoming an effective natural medicine for alleviating many non-communicable lifestyle-related diseases. The present study was conducted to evaluate the antioxidant and antitoxicant properties of a foodborne probiotic Bifidobacterium sp. MKK4 and its rice fermented beverage. The extracts of culture broth, whole cells, fermented beverage, and it’s heat-inactivated counterparts subjected to in vitro antioxidant/antiradical assays by DPPH, ABTS, and FRAP analysis. Except for heat-inactivated states, all samples exhibited strong antioxidant activity. In the experimental rat model, both Bifidobacterium sp. MKK4 and its rice fermented beverage significantly prevented arsenic toxicity by inducing a higher level of superoxide dismutase (SOD), catalase (CAT), reduced glutathione and preventing lipid peroxidation (LPO) and DNA fragmentation, and transmembrane mitochondrial potential. Besides, the organism supported systematic protection by improving the level of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, lactate dehydrogenase, C-reactive protein, urea, creatinine, and uric acid. The inherent antioxidant nature of the isolate can be exploited as an ingredient in functional food and an effective antidote against arsenic toxicity.

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Availability of data and material

The authors confirm that the data supporting the findings of this study are available within the article.

Code availability

The datasets generated during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are thankful to the Department of Science and Technology, Government of West Bengal, West Bengal, India for financial assistance [1169(Sanc.)/ST/P/S&T/1G-12/2015] for fellowships and funding. However, funder had no role in experimental designing or in manuscript preparation.

Funding

Department of Science and Technology, Government of West Bengal, West Bengal, India for financial assistance [1169(Sanc.)/ST/P/S&T/1G-12/2015] for fellowships and funding.

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Contributions

The trial was conceived by MR and PH. KM designed the study. MR and PH conducted the research. SS and KM prepared the manuscript. KM had the primary responsibility of the final content. All authors read and approved the final manuscript.

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Correspondence to Keshab Chandra Mondal.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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The animal experiment was carried out by taken Vidyasagar University Animal Ethical clearance (ICE/7-8/6-8/16 dt. 26.08.2016).

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Not applicable (as experiments carried out with experimental animal by taking appropriate ethical clearance).

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Ray, M., Hor, P., Singh, S.N. et al. Multipotent antioxidant and antitoxicant potentiality of an indigenous probiotic Bifidobacterium sp. MKK4. J Food Sci Technol (2021). https://doi.org/10.1007/s13197-021-04975-z

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Keywords

  • Bifidobacterium
  • Rice-fermented beverages
  • Arsenic toxicity
  • Antioxidant
  • Antitoxicant