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Targeted phenolic profiling of Sauvignon blanc and Shiraz grapes grown in two regions of India by liquid chromatography-tandem mass spectrometry

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Abstract

The phenolic compounds play an important role in production of quality grapes and wines. The current investigation focused on optimization of an extraction method for targeted analysis of 33 phenolic compounds in grapes by liquid chromatography tandem mass spectrometry (LC–MS/MS). The optimized method was successfully used for phenolic profiling of two wine grape varieties, Sauvignon blanc (white) and Shiraz (red) originated from Pune and Nasik regions of Maharashtra State, India. The optimized sample preparation procedure involved liquid–liquid extraction with acidified methanol by vortexing for 2 min followed by analysis on LC–MS/MS. The limit of quantification of the targeted compounds was in the range of 29 to 411 µg/L. The results indicated that skin of both varieties contained the highest amount of flavonols (69.47 ± 14.74 mg/kg in Sauvignon blanc and 129.47 ± 10.05 mg/kg in Shiraz) compared to pulp. The highest amounts of flavan-3-ols were present in grape seed collected from the Pune region (2016.84 ± 14.73 mg/kg in Sauvignon blanc and 1945.06 ± 32.69 mg/kg in Shiraz). The concentration of stilbenes was the highest in grape skin (0.13 ± 0.52 to 5.78 ± 5.45 mg/kg) compared to seed and pulp of both varities. Hydroxybenzoic acid (vanillin), hydroxycinnamic acid (p-coumaric acid) and anthocyanins (oenin, malvidin, cyanidin and kuromanin) were found only in Shiraz variety. The results of antioxidant activity (FRAP and DPPH assay) indicated the highest scavenging activity in seed (978.64 ± 56.23 to1133.38 ± 143.65 µMol TE/g DW FRAP and 594.93 ± 37.94 to 631.94 ± 56.45 µMol TE/g DW in DPPH). The phenolic contents in Sauvignon blanc and Shiraz grapes between Pune and Nasik regions did not have any significant difference.

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References

  • Arnous A, Makris DP, Kefalas P (2001) Effect of principle polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem 49:5736–5742

    Article  CAS  PubMed  Google Scholar 

  • Arts ICW, Hollman PCH (2005) Polyphenols and disease risk in epidemiologic studies1-4. Am J ClinNutr 81:317S–325S

    CAS  Google Scholar 

  • Benmeziane F, Djamai R, Cadot Y, Seridi R (2014) Optimization of extraction parameters of phenolic compounds from Algerian fresh table grapes (Vitisvinifera). Food Res Int 21:1025–1029

    Google Scholar 

  • Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  • Blackhall ML, Berry R, Davies NW, Walls JT (2018) Optimized extraction of anthocyanins from Reid Fruits’ Prunusavium ‘Lapins’ cherries. Food Chem 256:280–285

    Article  CAS  PubMed  Google Scholar 

  • Bonilla EP, Akoh CC, Sellapan S, Krewer G (2003) Phenolics content an antioxidant capacity of Muscadine grapes. J Agric Food Chem 51:5497–5503

    Article  CAS  Google Scholar 

  • Braidot E, Zancani M, Petrussa E, Peresson C, Bertolini A, Patio S, Macrì F (2008) Transport and accumulation of flavonoids in grapevine (Vitisvinifera L.). Plant Signal Behav 3:626–632

    Article  PubMed  PubMed Central  Google Scholar 

  • Butkhupl L, Chowtivannakul S, Gaensakoo R, Prathepha P, Samappito S (2010) Study of the phenolic composition of Shiraz red grape cultivar (Vitisvinfera L.) Cultivated in North-eastern Thailand and its antioxidant and antimicrobial activity. S Afr J Enol 31:89–98

    Google Scholar 

  • Dejan G, Vele T, Milovan V, Ljubodrag V, Vlatka V, Slobodan M (2010) Polyphenolic compounds in seeds from some grape cultivars grown in Serbia. J Serb Chem Soc 75:1641–1652

    Article  CAS  Google Scholar 

  • Gatto P, Vrhovsek U, Muth J, Segala C, Romualdi C, Fontana P, Pruefer D, Stefanini M, Moser C, Mattivi F, Velasco R (2008) Ripening and genotype control stilbene accumulation in healthy grapes. J Agric Food Chem 56:11773–11785

    Article  CAS  PubMed  Google Scholar 

  • Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719–1726

    Article  CAS  Google Scholar 

  • Heredia FJ, Francia-Aricha EM, Rivas-Gonzalo JC, Vicario IM, Santos-Buelga C (1998) Chromatic characterization of anthocyanin’s from red grapes - I. pH effect. Food Chem 63:491–498

    Article  CAS  Google Scholar 

  • Hismath I, Want Aida WM, Ho CW (2011) Optimization of extraction conditions for phenolic compounds from neem (Azadirachtaindica) leaves. Int Food Res J 18:931–939

    CAS  Google Scholar 

  • Jaitz L, Siegl K, Eder R, Rak G, Abranko L, Koellensperger G, Hann S (2010) LC-MS/MS analysis of phenols for classification of red wine according to geographic origin, grape variety and vintage. Food Chem 122:366–372

    Article  CAS  Google Scholar 

  • Jayaprakasha GK, Selvi T, Sakariah KK (2003) Antibacterial and antioxidant activities of grape (Vitisvnifera) seed extracts. Food Res Int 36:117–122

    Article  CAS  Google Scholar 

  • Jin Zan-Min, He Jian-Jun, Bi He-Qiong, Cui Xiang-Yun, Duan Chang-Qing (2009) Phenolic compound profiles in berry skins from nine red wine grape cultivars in northwest china. Molecules 14:4922–4935

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Juana M, Francisco P, Pilar Z (2010) Antioxidant activity and phenolic composition of organic and conventional grapes and wines. J Food Compos Anal 23:569–574

    Article  CAS  Google Scholar 

  • Li Zheng, Pan Qiuhong, Cui Xiangyun, Duan Changqing (2010) Optimization on anthocyanins extraction from wine grape skins using orthogonal test design. Food Sci Bio technol 19:1047–1053

    CAS  Google Scholar 

  • Mira de Orduña R (2010) Climate change associated effects on grape and wine quality and production. Food Res Int 43:1844–1855

    Article  CAS  Google Scholar 

  • Monagas M, Bartolome B, Gomez-Cordoves C (2005) Updated knowledge about the presence of phenolic compounds in wine. Crit Rev Food Sci Nutr 45:85–118

    Article  CAS  PubMed  Google Scholar 

  • Naczk M, Shahidi F (2004) Extraction and analysis of phenolics in food. J Chromatogr A 1054:95–111

    Article  CAS  PubMed  Google Scholar 

  • Pang B, Zhu Y, Lu L, Gu F, Chen H (2016) The applications and features of liquid chromatography-mass spectrometry in the analysis of traditional chinese medicine. Evid Based Complement Altern Med 2016:3837270

    Google Scholar 

  • Rockenbach II, Gonzaga LV, Rizelio VM, Goncalves A, Genovese MI, Fett R (2011) Phenolic compounds and antioxidant activity of seed and skin extracts of red grape (Vitisvinifera and Vitislabrusca) pomace from Brazilian winemaking. Food Res Int 44:897–901

    Article  CAS  Google Scholar 

  • Rodriguez MR, Romero Peces R, Chacon Vozmediano JL, MartinezGascuena J, Garcia Romero E (2006) Phenolic compounds in skins and seeds of ten grape Vitisvinifera varieties grown in a warm climate. J Food Compos Anal 19:687–693

    Article  CAS  Google Scholar 

  • Rolle L, Guidoni S (2007) Color and anthocyanin evaluation of red winegrapes CIE L*, a*, b* parameters. J Int Sci Vigne Vin 41:193–201

    CAS  Google Scholar 

  • Roobha JJ, Saravanakumar M, Aravindhan KM, Devi PS (2011) The effect of light, temperature, pH on stability of anthocyanin pigments in Musa acuminata bract. Res Plant Biol 1:05–12

    Google Scholar 

  • Salamon I, Mariychuk R, Grulova D (2015) Optimal extraction of pure anthocyanins from fruits of Sambucusnigra. Acta Hortic 1061:73–78

    Article  Google Scholar 

  • Sanchez-Moreno C (2002) Review: methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 8:121–137

    Article  CAS  Google Scholar 

  • Sellappan S, Akoh CC (2002) Flavonoids and antioxidant capacity of Georgia-Grown vidalia onions. J Agric Food Chem 50:5338–5342

    Article  CAS  PubMed  Google Scholar 

  • Tarola AM, Milano F, Giannetti V (2007) Simultaneous determination of phenolic compounds in red wines by HPLC-UV. Anal Lett 40:2433–2445

    Article  CAS  Google Scholar 

  • Teixeira A, Eiras-Dias J, Castellarin SD, Gerós H (2013) Berry phenolics of grapevine under challenging environments. Int J Mol Sci 14:18711–18739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Uma DB, Ho CW, Wan Aida WM (2010) Optimization of extraction parameters of total phenolic compounds from henna (Lawsoniainermis) Leaves. Sains Malays 39:119–128

    CAS  Google Scholar 

  • Wissam Z, Ghada B, Wassim A, Warid K (2012) Effective extraction of polyphenols and proanthocyanidins from pomegranate’s peel. Int J Pharm Pharm Sci 4:675–682

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the funding supports from Indian Council for Agricultural Research (ICAR), New Delhi, India.

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Authors and Affiliations

  1. National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. Manjri Farm, Pune, Maharashtra, 412307, India

    Kavita Savalekar, T. P. Ahammed Shabeer, Zareen Khan, Dasharath Oulkar, Prachi Jain, Chanchal Patil & Kaushik Banerjee

  2. Department of Agrochemicals and Pest Management, Shivaji University, Kolhapur, 416004, India

    Kavita Savalekar

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  1. Kavita Savalekar
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  2. T. P. Ahammed Shabeer
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Correspondence to Kaushik Banerjee.

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Savalekar, K., Ahammed Shabeer, T.P., Khan, Z. et al. Targeted phenolic profiling of Sauvignon blanc and Shiraz grapes grown in two regions of India by liquid chromatography-tandem mass spectrometry. J Food Sci Technol 56, 3300–3312 (2019). https://doi.org/10.1007/s13197-019-03802-w

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