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Inhibitory effects of acetophenone or phenylethyl alcohol as fumigant to protect soybean seeds against two aflatoxin-producing fungi

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Abstract

The antifungal activity of acetophenone and phenylethyl alcohol prevents seed contamination by Aspergillus flavus TISTR 3041 and A. parasiticus TISTR 3276. Their effects on seed germination were investigated. In vitro results showed that 100 µL L−1 acetophenone completely inhibited (by 100%) growth, conidial germination, and sporulation of the two aflatoxin-producing fungi, while phenylethyl alcohol showed only weak inhibitory activity even at 1000 µL L−1. Exposure to acetophenone at 100 µL L−1 for 6 h could completely kill (100% death) both fungal strains, while phenylethyl alcohol showed much lower efficacy (53.12%). In vivo results revealed that fumigation with 100 µL L−1 acetophenone for 24 h completely controlled (100%) A. flavus TISTR 3041 on soybean seeds during a 14-day test but exhibited weak efficacy on A. parasiticus TISTR 3276 (31.77%). Phenylethyl alcohol (1000 µL L−1) demonstrated weak inhibitory effect against both strains. The two volatile compounds had no adverse effects on seed germination. SEM confirmed that acetophenone could completely inhibit conidia germination, and abnormal growth of both fungal strains was observed. Thus, acetophenone has high potential to protect soybean seeds against aflatoxin-producing fungi.

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Acknowledgements

This research work was financially supported by the Agricultural Research Development Agency (Public Organization) (PRP5905021490) and Thailand Research Fund (RTA6080010).

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Authors and Affiliations

  1. College of Innovation and Management, Songkhla Rajabhat University, Songkhla, 90000, Thailand

    Sawai Boukaew

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90112, Thailand

    Poonsuk Prasertsan

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  1. Sawai Boukaew
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Correspondence to Sawai Boukaew.

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Boukaew, S., Prasertsan, P. Inhibitory effects of acetophenone or phenylethyl alcohol as fumigant to protect soybean seeds against two aflatoxin-producing fungi. J Food Sci Technol 55, 5123–5132 (2018). https://doi.org/10.1007/s13197-018-3458-6

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