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Antiglycation and antioxidant activities of mogroside extract from Siraitia grosvenorii (Swingle) fruits

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Abstract

Siraitia grosvenorii (Swingle) is one kind of medical and edible plants with various health-promoting properties. Recently, its hypoglycemic and antidiabetic activities have been reported, but the underlying mechanism remains to be explored. The current study was aimed to investigate the antioxidant and antiglycation activities of mogroside extract (MGE) from Siraitia grosvenorii (Swingle). The results showed that compared to glycated BSA, MGE at middle (125 μg/mL) and high dose (500 μg/mL) significantly inhibited BSA glycation evidenced by decreased fluorescent AGEs formation, protein carbonyls and Nε-(carboxymethyl) lysine (CML) level at 500 μg/mL by 58.5, 26.7 and 71.2%, respectively. Additionally, the antiglycative activity of MGE (500 μg/mL) was comparable to aminoguanidine (AG) at the equal concentration. However, the inhibitory effect of MGE on glycation-induced increase of fructosamine level and decrease of thiol level was not remarkable. MGE was a potent peroxide radicals scavenger (851.8 μmol TE/g), moderate DPPH and ABTS radicals scavenger with IC50 1118.1 and 1473.2 μg/mL, respectively, corresponding to positive controls ascorbic acid of IC50 9.6 μg/mL, and trolox of IC50 47.9 μg/mL, respectively, and mild reducing power. These findings suggest that MGE may serve as a new promising antiglycative agent against diabetic complications by inhibiting protein glycation and glycoxidation.

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Abbreviations

1-DMF:

1-Deoxy-1-morpholino-fructose

3-DG:

3-Deoxyglucosone

AAPH:

2,2′-Azobis (2-amidinopropane) dihydrochloride

ABTS:

2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)

AG:

Aminoguanidine

AGEs:

Advanced glycation end products

ANOVA:

Analysis of variance

AUC:

Area under curve

BSA:

Bovine serum albumin

CML:

Nε-(carboxymethyl)lysine

DNPH:

2,4-Dinitrophenylhydrazine

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

DPTC:

4,5-Dimethyl-3-phenacylthiazolium chloride

DTNB:

5,5′-Dithiobis-(2-nitrobenzoic acid)

ELISA:

Enzyme-linked immunosorbent assay

FI:

Fluorescence intensity

GO:

Glyoxal

GRAS:

Generally recognized as safe

MGE:

Mogroside extract

MGO:

Methylglyoxal

NBT:

4-Nitro blue tetrazolium

ORAC:

Oxygen radical absorbance capacity

PBS:

Phosphate buffered saline

ROS:

Reactive oxygen species

SD:

Standard deviation

TCA:

Trichloroacetic acid

Trolox:

6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid

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Acknowledgements

The authors thank Guilin Layn Natural Ingredients Corp. for generously providing MGE. This work was funded by National Natural Science Foundation of China (No. 31171780), Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (No. 20171033) and general program from Department of Education of Zhejiang Province, China (Y201738544).

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Authors and Affiliations

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Hesheng Liu & Zhida Sun

  2. Shanghai OPM Biosciences Co., Ltd., Shanghai, 201321, People’s Republic of China

    Chengcheng Wang

  3. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People’s Republic of China

    Hesheng Liu, Xiangyang Qi & Jian Zou

  4. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, 100048, People’s Republic of China

    Xiangyang Qi

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  1. Hesheng Liu
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  5. Zhida Sun
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Correspondence to Xiangyang Qi.

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Liu, H., Wang, C., Qi, X. et al. Antiglycation and antioxidant activities of mogroside extract from Siraitia grosvenorii (Swingle) fruits. J Food Sci Technol 55, 1880–1888 (2018). https://doi.org/10.1007/s13197-018-3105-2

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  • DOI: https://doi.org/10.1007/s13197-018-3105-2

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