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Optimization of conditions for probiotic curd formulation by Enterococcus faecium MTCC 5695 with probiotic properties using response surface methodology

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Abstract

Enterococcus faecium MTCC 5695 possessing potential probiotic properties as well as enterocin producing ability was used as starter culture. Effect of time (12–24 h) and inoculum level (3–7 % v/v) on cell growth, bacteriocin production, antioxidant property, titrable acidity and pH of curd was studied by response surface methodology (RSM). The optimized conditions were 26.48 h and 2.17%v/v inoculum and the second order model validated. Co cultivation studies revealed that the formulated product had the ability to prevent growth of foodborne pathogens that affect keeping quality of the product during storage. The results indicated that application of E. faecium MTCC 5695 along with usage of optimized conditions attributed to the formation of highly consistent well set curd with bioactive and bioprotective properties. Formulated curd with potential probiotic attributes can be used as therapeutic agent for the treatment of foodborne diseases like Traveler’s diarrhea and gastroenteritis which thereby help in improvement of bowel health.

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Acknowledgments

NB thankfully acknowledges Council of Scientific and Industrial Research (CSIR) for funding this work through Encouraging & Motivating World class Exploratory Research (EMPOWER) scheme. VR thanks University Grants Commission (UGC), Govt. of India for the senior research fellowship. Authors place on record their thanks to Director, CFTRI for encouragement and permission to publish the work.

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Authors and Affiliations

  1. Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Vrinda Ramakrishnan, Louella Concepta Goveas & Prakash M. Halami

  2. Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Maya Prakash

  3. Department of Meat, Fish and Poultry Technology, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Bhaskar Narayan

Authors
  1. Vrinda Ramakrishnan
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  2. Louella Concepta Goveas
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  3. Maya Prakash
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  4. Prakash M. Halami
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  5. Bhaskar Narayan
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Correspondence to Bhaskar Narayan.

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Ramakrishnan, V., Goveas, L.C., Prakash, M. et al. Optimization of conditions for probiotic curd formulation by Enterococcus faecium MTCC 5695 with probiotic properties using response surface methodology. J Food Sci Technol 51, 3050–3060 (2014). https://doi.org/10.1007/s13197-012-0821-x

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  • DOI: https://doi.org/10.1007/s13197-012-0821-x

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