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Cytochrome P450 Genes (CYP2E1 and CYP1A1) Variants and Susceptibility to Chronic Hepatitis B Virus Infection

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Abstract

Hepatitis B virus (HBV) infection is a worldwide health concern which is associated with significant morbidity and mortality. Both viral and host factors have a significant effect on infection, replication and pathogenesis of HBV. The aim of this study was to investigate the effect of CYP2E1 and CYP1A1 genetic variants on susceptibility to HBV. 143 individuals including 54 chronic HBV patients and 89 healthy controls were enrolled in the genotyping procedure. rs2031920 and rs3813867 at CYP2E1 as well as rs4646421 and rs2198843 at CYP1A1 loci were studied in all subjects using PCR–RFLP (restriction fragment length polymorphism) analysis. Both variants at CYP2E1 locus were monomorphic in all studied subjects. Genotype frequency of rs4646421 was significantly different between chronic HBV patients and healthy blood donors (P = 0.04, OR 4.31; 95% CI 1.04–17.7). Furthermore, individuals carrying at least one C allele (CC or CT genotypes) for rs4646421 seemed to have a decrease risk of hepatitis in comparison with TT genotype (P = 0.039). Our results showed a relationship between rs4646421 TT genotype (rare genotype) and the risk for developing chronic HBV infection (four times higher). Further studies are needed to examine the role of CYP1A1 polymorphism in susceptibility to chronic HBV infection.

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Acknowledgements

Authors would like to acknowledge all people who consented to participate to this study.

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Authors and Affiliations

  1. Cellular and Molecular Department, North Research Center, Pasteur Institute of Iran, Amol, Iran

    Sadegh Fattahi, Mohsen Asouri, Maryam Lotfi, Galia Amirbozorgi & Haleh Akhavan-Niaki

  2. Department of Infectious Diseases, Imam Reza Hospital, Amol, Iran

    Mohammad Karimi Alivije

  3. Department of Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Farhang Babamahmoodi

  4. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

    Masomeh Bayani & Mahmoud Sadeghi Haddad Zavareh

  5. Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

    Haleh Akhavan-Niaki

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  1. Sadegh Fattahi
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  2. Mohammad Karimi Alivije
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  8. Galia Amirbozorgi
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  9. Haleh Akhavan-Niaki
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Correspondence to Haleh Akhavan-Niaki.

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Fattahi, S., Karimi Alivije, M., Babamahmoodi, F. et al. Cytochrome P450 Genes (CYP2E1 and CYP1A1) Variants and Susceptibility to Chronic Hepatitis B Virus Infection. Ind J Clin Biochem 33, 467–472 (2018). https://doi.org/10.1007/s12291-017-0698-6

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  • DOI: https://doi.org/10.1007/s12291-017-0698-6

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