Indian Journal of Clinical Biochemistry

, Volume 33, Issue 2, pp 218–221 | Cite as

Plasma Neutrophil Elastase, α1-Antitrypsin, α2-Macroglobulin and Neutrophil Elastase–α1-Antitrypsin Complex Levels in patients with Dengue Fever

  • Mamatha Kunder
  • V. Lakshmaiah
  • A. V. Moideen Kutty
Original Article


Dengue fever (DF) is characterized by systemic inflammatory response including neutrophil activation leading to uncontrolled elastase activity. This study was aimed to measure the activity of plasma neutrophil elastase (NE), its endogenous inhibitors α1-antitrypsin (α1-AT) and α2-macroglobulin (α2-MG) and elastase in complex with α1-AT (NE–α1-AT complex) in DF. 50 dengue patients [39 DF and 11 dengue hemorrhagic fever (DHF)] and 52 healthy subjects were included in the study. NE was measured using N-succinyl-tri-alanine-p-nitroanilide as substrate. α1-AT, α2-MG and NE–α1-AT complex were estimated by ELISA. The result analysis indicated that the dengue patients had significantly higher elastase activity with significantly reduced inhibitor levels compared to controls. Between DF and DHF patients, DHF group had significantly higher elastase activity. In conclusion, significantly elevated NE and reduced inhibitors level in dengue fever indicate these parameters could be of significance in DF particularly for the assessment of progression of inflammatory processes.


Dengue fever Neutrophil elastase α1-Antitrypsin α2-Macroglobulin NE–α1-AT complex 


Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Association of Clinical Biochemists of India 2017

Authors and Affiliations

  1. 1.Department of BiochemistrySri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and ResearchTamaka, KolarIndia
  2. 2.Department of MedicineSri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and ResearchTamaka, KolarIndia

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