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Plasma Neutrophil Elastase, α1-Antitrypsin, α2-Macroglobulin and Neutrophil Elastase–α1-Antitrypsin Complex Levels in patients with Dengue Fever

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Abstract

Dengue fever (DF) is characterized by systemic inflammatory response including neutrophil activation leading to uncontrolled elastase activity. This study was aimed to measure the activity of plasma neutrophil elastase (NE), its endogenous inhibitors α1-antitrypsin (α1-AT) and α2-macroglobulin (α2-MG) and elastase in complex with α1-AT (NE–α1-AT complex) in DF. 50 dengue patients [39 DF and 11 dengue hemorrhagic fever (DHF)] and 52 healthy subjects were included in the study. NE was measured using N-succinyl-tri-alanine-p-nitroanilide as substrate. α1-AT, α2-MG and NE–α1-AT complex were estimated by ELISA. The result analysis indicated that the dengue patients had significantly higher elastase activity with significantly reduced inhibitor levels compared to controls. Between DF and DHF patients, DHF group had significantly higher elastase activity. In conclusion, significantly elevated NE and reduced inhibitors level in dengue fever indicate these parameters could be of significance in DF particularly for the assessment of progression of inflammatory processes.

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References

  1. Juffrie M, Van Der Meer GM, Hack CE, Haasnoot K, Sutaryo Veerman AJP, et al. Inflammatory mediators in dengue virus infection in children: interleukin-8 and its relationship to neutrophil degranulation. Infect Immun. 2000;68:702–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Petrina T, David CL, Tun KT, Carol YC, Tun-Linn T, Joshua GW, et al. A prospective case-control study to investigate retinal microvascular changes in acute dengue infection. Sci Rep. 2015;5:17183. doi:10.1038/srep17183.

    Article  Google Scholar 

  3. Amanda LA, Alexander MA, Claudine SB, Stuart MP, Jillian MC. Dengue virus-induced inflammation of the endothelium and the potential roles of sphingosine kinase-1 and microRNAs. Mediators Inflamm. 2015. doi:10.1155/2015/509306.

    Google Scholar 

  4. Jih-Jin T, Kulkanya C, Yee SL, Guey CP. Role of neutrophils in dengue patients: clearance of dengue virus [Abstract]. Frontiers in immunology and inflammation: from molecules to disease 2012;A–20.

  5. Baggiolini MAW, Kunkel SL. Neutrophil-activating peptide-1/interleukin-8, a novel cytokine that activates neutrophils. J Clin Investig. 1989;84:1045–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Jeanne AP. Dengue virus infection: predictors for severe dengue. Acta Med Indones. 2011;43:129–135.

    Google Scholar 

  7. Hiroto M, Katsunori Y, Hiroshi M, Kenji T, Masamitsu N, Shigeru K. Association of plasma neutrophil elastase levels with other inflammatory mediators and clinical features in adult patients with moderate and severe pneumonia. Respir Med. 2007;101:1521–8.

    Article  Google Scholar 

  8. Smedly LA, Tonnesen MG, Sandhaus RA, Haslett C, Guthrie LA, Johnston RB, et al. Neutrophil mediated injury to endothelial cells: enhancement by endotoxin and essential role of neutrophil elastase. J Clin Investig. 1986;77:1233–43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Bieth J, Spiess B, Wermuth CG. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochem Med. 1974;11:350–7.

    Article  CAS  PubMed  Google Scholar 

  10. Peifang S, Karolis B, Subhamoy P, Zhaodong L, Shuenn-jue W, Charmagne B, et al. Infection and activation of human peripheral blood monocyte by dengue viruses through the mechanism of antibody-dependent enhancement. Virology. 2011;421:245–52.

    Article  Google Scholar 

  11. Shanley B, Zhihua K, Huiyuan C, Matthew Q, Robert CR, Jacob JS, et al. Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. J Virol. 2007;81:13325–34.

    Article  Google Scholar 

  12. Soundravally R, Sankar P, Bobby Z, Hoti SL. Oxidative stress in severe dengue viral infection: association of thrombocytopenia with lipid peroxidation. Platelets. 2008;19:447–54.

    Article  CAS  PubMed  Google Scholar 

  13. Gil L, Martinez G, Tapanes R, Castro O, Gonalez D, Bernardo L, et al. Oxidative stress in adult dengue patients. Am J Trop Med Hyg. 2004;71:652–7.

    CAS  PubMed  Google Scholar 

  14. Jiro F, Hiroyuki N, Yoshifumi Y, Yusufumi Y, Taiichi S, Shozo I. Elevation of plasma truncated elastase α1-proteinase inhibitor complexes in patients with inflammatory lung diseases. Chest. 1992;102:129–34.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Sri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, Karnataka, 563103, India

    Mamatha Kunder & A. V. Moideen Kutty

  2. Department of Medicine, Sri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, Karnataka, India

    V. Lakshmaiah

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  1. Mamatha Kunder
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  2. V. Lakshmaiah
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  3. A. V. Moideen Kutty
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Correspondence to A. V. Moideen Kutty.

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Kunder, M., Lakshmaiah, V. & Moideen Kutty, A.V. Plasma Neutrophil Elastase, α1-Antitrypsin, α2-Macroglobulin and Neutrophil Elastase–α1-Antitrypsin Complex Levels in patients with Dengue Fever. Ind J Clin Biochem 33, 218–221 (2018). https://doi.org/10.1007/s12291-017-0658-1

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  • DOI: https://doi.org/10.1007/s12291-017-0658-1

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