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A Comparison Between Shaker and Bioreactor Performance Based on the Kinetic Parameters of Xanthan Gum Production

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Abstract

Xanthan gum production was studied using sugarcane broth as the raw material and batch fermentation by Xanthomonas campestris pv. campestris NRRL B-1459. The purpose of this study was to optimize the variables of sucrose, yeast extract, and ammonium nitrate concentrations and to determine the kinetic parameters of this bioreaction under optimized conditions. The effects of yeast extract and ammonium nitrate concentrations for a given sucrose concentration (12.1–37.8 g L−1) were evaluated by central composite design to maximize the conversion efficiency. In a bioreactor, the maximum conversion efficiency was achieved using 27.0 g L−1 sucrose, 2.7 g L−1 yeast extract, and 0.9 g L−1 NH4NO3. This point was assayed in a shaker and in a bioreactor to compare bioreaction parameters. These parameters were estimated by the unstructured kinetic model of Weiss and Ollis (Biotechnol Bioeng 22:859–873, 1980) to determinate the yields (Y P/S), the maximum growth specific rate (μ max), and the saturation cellular concentration (X*). The parameters of the model (μ max, X*, m, λ, α, and β) were obtained by nonlinear regression. For production of xanthan gum in a shaker, the values of μ max and Y P/S obtained were 0.119 h−1 and 0.34 g g−1, respectively, while in a bioreactor, they were 0.411 h−1 and 0.63 g g−1, respectively.

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Acknowledgments

The authors wish to thank the CNPq and FAPEMIG—Brazil—for its financial support.

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Authors and Affiliations

  1. Faculty of Chemical Engineering, Uberlândia Federal University, P.O. Box 593, 38400-902, Uberlândia, Minas Gerais, Brazil

    S. Faria, P. A. Vieira, M. M. Resende & V. L. Cardoso

  2. School of Chemical, Rio de Janeiro Federal University, P.O. Box 68502, 21945-970, Rio de Janeiro, Rio de Janeiro, Brazil

    F. P. França

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  1. S. Faria
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  2. P. A. Vieira
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Correspondence to V. L. Cardoso.

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Faria, S., Vieira, P.A., Resende, M.M. et al. A Comparison Between Shaker and Bioreactor Performance Based on the Kinetic Parameters of Xanthan Gum Production. Appl Biochem Biotechnol 156, 45–58 (2009). https://doi.org/10.1007/s12010-008-8485-8

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