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Resistance to greenbugs in the sorghum nested association mapping population

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Abstract

The greenbug, Schizaphis graminum, is a serious pest of sorghum (Sorghum bicolor). For the past several decades, resistant sorghum hybrids have been used to control greenbug populations. However, the durability of plant resistance is frequently challenged by evolution of new greenbug biotypes, and there is a continuous need for screening of resistant germplasm for its effective management in the field. Natural variation in sorghum plants/populations provides distinct approaches to identify novel sources of resistance against greenbugs. In this study, we used the recently developed sorghum nested association mapping (NAM) population parental lines to understand sources of sorghum resistance to greenbugs. Using choice and no-choice assays, we have identified SC265 and Segaolane as the resistant and susceptible lines, respectively, to greenbugs compared to the wild-type plants. The Electrical Penetration Graph (EPG) analysis revealed that the greenbugs spent significantly lesser time in the xylem and sieve element phases while feeding on the resistant NAM parental line, SC265, compared to the susceptible (Segaolane) and wild-type (RTx430) sorghum lines. In addition, the EPG results indicated that there is no significant difference in the time to first probe, time to reach first sieve element, pathway phase, and non-probing phase among the three sorghum plants, which suggests that the resistance factors present in the vascular tissues of the resistant line (SC265) potentially contribute to the resistance mechanisms against greenbugs. Overall, SC265 NAM parental line showed a combination of antixenotic and antibiotic-mediated resistance mechanisms against greenbugs, whereas the susceptible line Segaolane displayed the least resistance to greenbugs.

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Acknowledgements

We thank Dr. Kyle Koch (University of Nebraska-Lincoln, USA) for providing the greenbug colony. Braden Wojahn was supported by Undergraduate Creative Activities and Research Experience (UCARE) funds from the University of Nebraska-Lincoln, USA. This work was partially supported by the Nebraska Agricultural Experiment Station with funding from the Hatch Act (Accession # 1007272) through the USDA National Institute of Food and Agriculture to Joe Louis and funds from USDA-ARS (58-3042-6-070).

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Authors and Affiliations

  1. Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA

    Sajjan Grover, Braden Wojahn, Suresh Varsani & Joe Louis

  2. Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA

    Scott E. Sattler

  3. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA

    Joe Louis

  4. Wheat, Sorghum, and Forage Research Unit, USDA-ARS, Lincoln, NE, 68583, USA

    Scott E. Sattler

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  1. Sajjan Grover
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Grover, S., Wojahn, B., Varsani, S. et al. Resistance to greenbugs in the sorghum nested association mapping population. Arthropod-Plant Interactions 13, 261–269 (2019). https://doi.org/10.1007/s11829-019-09679-y

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