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Optimization and analysis of microwave-assisted extraction of bioactive compounds from Mimosa pudica L. using RSM & ANFIS modeling

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Abstract

The central composite rotatable design (CCRD) based response surface methodology (RSM) and adaptive neuro-fuzzy inference system (ANFIS) statistical methodology was used to design and identify highly efficient extraction process parameters to get high yield of bioactive compound from Mimosa pudica L. In general, many of the process parameters are need to be effectively involved to maximize the yield of bioactive compounds. In this relation, the independent process parameters such as methanol concentration (X 1), microwave power (X 2), irradiation temperature (X 3) and irradiation time (X 4) was chosen in the process of microwave assisted extraction (MAE). The observed studied parametes were produced effective results in the range of 60–85% methanol concentration, 15–25% microwave power, 40–60 °C irradiation temperature and irradiation time 10–15 min. Moreover the optimal yields of TPC and TFC are 635–640 mg gallic acid equivalents (GAE)/g and 61.53–61.76 mg rutin equivalents (RU)/g of extract, and their antioxidant activities are 68.7–72.6% DPPHsc, 76.1–76.86% ABTSsc and FRAB value of 65.24–66.94 µg mol (Fe (II))/g could be obtained for specific optimized process variables. Further, the bioactive compound mimopudine was identified through high performance liquid chromatography (HPLC) in the obtained extract.

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Acknowledgements

We gratefully thank the Chancellor, Vice-chancellor and Directors of Kalasalingam University, Krishnankoil, India for research fellowships and utilizing research facilities. We thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiwics, Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warszawa, Poland, for their prompt support and suggestions.

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Authors and Affiliations

  1. Department of Pharmaceutics, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, Virudhunagar, 626126, India

    Vinothapooshan Ganesan

  2. Department of Computer Science and Engineering, Kalasalingam Institute of Technology, Anand Nagar, Krishnankoil, Virudhunagar, 626126, India

    Vijaya Gurumani

  3. Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Anand Nagar, Krishnankoil, Virudhunagar, 626126, India

    Selvaraj Kunjiappan & Balasubramanian Somasundaram

  4. Department of Pharmaceutical Chemistry, Karavali College of Pharmacy, Vamanjoor, Mangalore, 575028, Karnataka, India

    Theivendran Panneerselvam

  5. National Cyber Defence Research Centre, Kalasalingam University, Anand Nagar, Krishnankoil, Virudhunagar, 626126, India

    Suthendran Kannan

  6. Department of Biotechnology, Kalasalingam University, Anand Nagar, Krishnankoil, Virudhunagar, 626126, India

    Anindita Chowdhury

  7. MNR College of Pharmacy, Fasalwadi, Sangareddy, Telangana, 502294, India

    Govindraju Saravanan

  8. Department of Chemical Engineering, Jadavpur University, Kolkata, 700032, India

    Chiranjib Bhattacharjee

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  1. Vinothapooshan Ganesan
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  2. Vijaya Gurumani
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Correspondence to Selvaraj Kunjiappan.

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Ganesan, V., Gurumani, V., Kunjiappan, S. et al. Optimization and analysis of microwave-assisted extraction of bioactive compounds from Mimosa pudica L. using RSM & ANFIS modeling. Food Measure 12, 228–242 (2018). https://doi.org/10.1007/s11694-017-9634-y

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  • DOI: https://doi.org/10.1007/s11694-017-9634-y

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