An approach for extraction, purification, characterization and quantitation of acylated-anthocyanins from Nitraria tangutorun Bobr. fruit

  • Jun Sang
  • Qun Ma
  • Bing Li
  • Cui-qin Li
Original Paper


In the present study, a systematic approach for extraction, purification and analysis of acylated-anthocyanins from Nitraria tangutorun Bobr. fruit was explored. Six acylated-anthocyanins in N. tangutorun fruit were identified by HPLC-MS/MS, and a rapid and efficient HPLC-DAD method was developed to analyze the acylated-anthocyanins. Ultrasonic-assisted extraction conditions of acylated-anthocyanins were optimized using response surface methodology, extraction at 70 °C for 32 min using 70% methanol solution (0.1% HCl, v/v) rendered an extract with 80.37 ± 2.66 mg/100 g of cyanidin-3-O-(trans-p-coumaroyl)-diglucoside and 97.88 ± 4.06 mg/100 g of total acylated-anthocyanins. Nine macroporous resins were investigated for preliminary purification of acylated-anthocyanins. According to the static/dynamic adsorption and desorption tests, XDA-6 macroporous resin exhibited the maximum potential for preparing acylated-anthocyanins. The purity of cyanidin-3-O-(trans-p-coumaroyl)-diglucoside (43.30 mg/g) in purified acylated-anthocyanins was 201.89 times of that of the extract (0.21 mg/g), and the purity of total acylated-anthocyanins increased from 0.36 to 56.44 mg/g. Besides, the stability (t 1/2) of cyanidin-3-O-(trans-p-coumaroyl)-diglucoside and total acylated-anthocyanins increased by more than five-fold after purification using XDA-6. The established methods of analysis, extraction and purification of acylated-anthocyanins were hopefully utilized in food industry.


Nitraria tangutorun Bobr. fruit Acylated-anthocyanins HPLC-DAD HPLC-ESI-MS/MS Macroporous resin 



Ultrasound-assisted extraction




Total acylated-anthocyanins




Bed volume


Reaction rate constant





The authors are grateful for financial support from the Fundamental Research Funds for the Central Universities (GK201303009) and Science and Technology Cooperation Project between Shaanxi Province and Qinghai Province (2014SJ-07).

Supplementary material

11694_2017_9615_MOESM1_ESM.docx (138 kb)
Supplementary material 1 (DOCX 137 KB)


  1. 1.
    K.S. Minioti, C.F. Sakellariou, N.S. Thomaidis, Anal. Chim. Acta 583, 103 (2007)CrossRefGoogle Scholar
  2. 2.
    M.M. Giusti, R.E. Wrolstad, Biochem. Eng. J. 14, 217 (2003)CrossRefGoogle Scholar
  3. 3.
    X. Pan, G. Shen, C. Peng, Acta Bot. Yunnanica 21, 287 (1999)Google Scholar
  4. 4.
    X. Yang, H. Zhang, X. Tang, Z. Liu, S. Yang, J. Ni, J. Zhu, World For. Res. 26, 64 (2013)Google Scholar
  5. 5.
    W. Ni, T. Gao, H. Wang, Y. Du, J. Li, C. Li, L. Wei, H. Bi, J. Ethnopharmacol. 150, 529 (2013)CrossRefGoogle Scholar
  6. 6.
    X. Peng, F. Gao, H. Lin, Y. Peng, Z. Peng, Guangdong Chem. 33, 49 (2006)Google Scholar
  7. 7.
    X. Zhao, P. Wang, Y. Tao, Y. Shao, L. Mei, Lishizhen Med. Mater. Med. Res. 22, 2426 (2011)Google Scholar
  8. 8.
    J. Zheng, H. Li, C.X. Ding, Y.R. Suo, L.S. Wang, H.L. Wang, Food Res. Int. 44, 2041 (2011)CrossRefGoogle Scholar
  9. 9.
    T. Ma, N. Hu, C. Ding, Q. Zhang, W. Li, Y. Suo, H. Wang, B. Bai, C. Ding, Food Chem. 194, 296 (2016)CrossRefGoogle Scholar
  10. 10.
    E. Roselló-Soto, C.M. Galanakis, M. Brncic, V. Orlien, F.J. Trujillo, R. Mawson, K. Knoerzer, B.K. Tiwari, F.J. Barba, Trends Food Sci. Technol. 42, 134 (2015)CrossRefGoogle Scholar
  11. 11.
    G.B. Celli, A. Ghanem, M.S. Brooks, Ultrason. Sonochem. 27, 449 (2015)CrossRefGoogle Scholar
  12. 12.
    W. Wang, J. Jung, E. Tomasino, Y. Zhao, LWT Food Sci. Technol. 72, 229 (2016)CrossRefGoogle Scholar
  13. 13.
    E. Espada-Bellido, M. Ferreiro-González, C. Carrera, M. Palma, C.G. Barroso, G.F. Barbero, Food Chem. 219, 23 (2017)CrossRefGoogle Scholar
  14. 14.
    K. Chafoor, Y.H. Choi, J.Y. Jeon, I.H. Jo, J. Agric. Food Chem. 57, 4988 (2009)CrossRefGoogle Scholar
  15. 15.
    M.A. Bezerra, R.E. Santelli, E.P. Oliveira, L.S. Villar, L.A. Escaleira, Talata 76, 965 (2008)Google Scholar
  16. 16.
    A.K. Sandhu, L. Gu, J. Agric. Food Chem. 61, 1441 (2013)CrossRefGoogle Scholar
  17. 17.
    X.L. Chang, D. Wang, B.Y. Chen, Y.M. Feng, S.H. Wen, P.Y. Zhan, J. Agric. Food Chem 60, 2368 (2012)CrossRefGoogle Scholar
  18. 18.
    H. Zheng, Agric. Eng. 6, 59 (2016)Google Scholar
  19. 19.
    J. Valls, S. Millán, M.P. Martí, E. Borràs, L. Arola, J. Chromatogr. A 1216, 7143 (2009)CrossRefGoogle Scholar
  20. 20.
    I.J. Košir, B. Lapornik, S. Andrenšek, A.G. Wondra, U. Vrhovšek, J. Kidric, Anal. Chim. Acta 513, 277 (2004)CrossRefGoogle Scholar
  21. 21.
    L. Yao, N. Zhang, C. Wang, C. Wang, J. Agric. Food Chem. 63, 3543 (2015)CrossRefGoogle Scholar
  22. 22.
    M. Özkan, A. Yemenicioglu, B. Cemeroglu, Food Res. Int. 38, 1015 (2005)CrossRefGoogle Scholar
  23. 23.
    C.M. Willemse, M.A. Stander, A. de Villiers, J. Chromatogr. A 1319, 127 (2013)CrossRefGoogle Scholar
  24. 24.
    B. Abad-García, L.A. Berrueta, S. Garmón-Lobato, B. Gallo, F. Vicente, J. Chromatogr. A 1216, 5398 (2009)CrossRefGoogle Scholar
  25. 25.
    J. Sang, J. Sang, Q. Ma, X. Hou, C. Li, Food Chem. 218, 386 (2017)CrossRefGoogle Scholar
  26. 26.
    S.B. Dhull, P. Kaur, S.S. Purewal, Resour. Eff. Technol. 2, 168 (2016)Google Scholar
  27. 27.
    R. Tabaraki, A. Nateghi, Ultrason. Sonochem. 18, 1279 (2011)CrossRefGoogle Scholar
  28. 28.
    M.D. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, P. Smith, Anal. Chem. 28, 350 (1956)CrossRefGoogle Scholar
  29. 29.
    S. Muñoz, M. Mestres, O. Busto, J. Guasch, Anal. Chim. Acta 628, 104 (2008)CrossRefGoogle Scholar
  30. 30.
    R.T. Huang, J.F. Lu, B.S. Inbaraj, B.H. Chen, J. Funct. Foods 12, 498 (2015)CrossRefGoogle Scholar
  31. 31.
    C. Jampani, K.S.M.S. Raghavarao, Sep. Purif. Technol. 141, 10 (2015)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life SciencesShaanxi Normal UniversityXi’anChina

Personalised recommendations