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Adhesion Behavior of Escherichia coli on Plasma-Sprayed Zn and Ag Co-incorporated Calcium Silicate Coatings with Varying Surface Roughness

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Abstract

Our previous work demonstrated the good biocompatibility and bactericidal activity of a plasma-sprayed Zn and Ag co-incorporated calcium silicate ceramic coating (Ag-Ca2ZnSi2O7), namely Ag-HT. In this work, we evaluated the influence of the surface roughness of Ag-HT coatings for Escherichia coli adhesion behavior, considering that surface topography is an important factor that regulates bacterial responses to biomaterials. Surface characteristics of as-sprayed and satin-finished Ag-HT coatings were investigated by scanning electron microscopy and measured using a surface roughness tester. Bacterial adhesion experiments showed that the number of viable E. coli cells on the as-sprayed or satin-finished coating surfaces was substantially lower than on the Ti-6Al-4V control surface, which can be explained by the antimicrobial effect of Ag and/or Zn. Moreover, the density of E. coli on the as-sprayed Ag-HT coating was considerably lower than on the satin-finished derivative. We attribute this difference to the fact that the as-sprayed coating surface was relatively smooth at a scale comparable to the size of an individual bacterium, while the satin-finished surface was slightly rough at this length scale and thus favored bacterial adhesion.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 51172264, 81300917), the Foundation of the Shanghai Biomaterial and Clinical Research Centre (Grant No. BMCRC2010004) and Municipal Commission of Health and Family Planning Foundation of Shanghai (20174Y0211).

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Authors and Affiliations

  1. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, People’s Republic of China

    Jiangming Yu, Ning Xu, Yanhai Xi, Mintao Xue, Weiheng Wang, Xiaodong Huang, Changkun Zheng & Xiaojian Ye

  2. Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200003, People’s Republic of China

    Kai Li

  3. Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, Shaanxi, 710069, People’s Republic of China

    Dai-xu Wei

  4. Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, People’s Republic of China

    Dai-xu Wei

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  1. Jiangming Yu
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  2. Ning Xu
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Correspondence to Dai-xu Wei, Kai Li or Xiaojian Ye.

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Yu, J., Xu, N., Xi, Y. et al. Adhesion Behavior of Escherichia coli on Plasma-Sprayed Zn and Ag Co-incorporated Calcium Silicate Coatings with Varying Surface Roughness. J Therm Spray Tech 27, 1428–1435 (2018). https://doi.org/10.1007/s11666-018-0800-5

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  • DOI: https://doi.org/10.1007/s11666-018-0800-5

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