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Selecting with markers linked to the PPVres major QTL is not sufficient to predict resistance to Plum Pox Virus (PPV) in apricot

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Abstract

Sharka is one of the most serious viral diseases affecting stone fruit species and, in apricot, resistance to its viral agent, the Plum Pox Virus (PPV), is conferred by one major quantitative trait locus (QTL), named PPVres for PPV resistance. Previous studies indicated that PPV-resistant cultivars and breeding progenies can be selected by using a set of SSR markers (named PGS) targeting the PPVres locus. However, before these markers can be employed for marker-assisted selection, they were validated in a wide range of genetic backgrounds and environments. We used a total of 11 mapping populations issued from three distinct environments to confirm that this marker set located within the QTL adequately predicted PPV resistance. In this study, we show that selection of PPV-resistant material based only on markers co-localizing with the PPVres major locus is not fully reliable. Indeed, genotype-phenotype discrepancies were observed depending on the progeny and the PPV-resistant/susceptible parents. While most of the PPV-resistant individuals displayed the resistant alleles, a significant number of PPV-susceptible individuals showed the same resistant haplotype. An effect of the PPV strain used for phenotyping was also demonstrated. We thus hypothesize that the presence of other factors or genes involved in the mechanism of resistance to sharka in apricot could explain these unexpected results. Our work indicates that the current PGS marker set is not broadly applicable for MAS and that marker-assisted breeding based on the sole PPVres locus is not sufficient to unambiguously select PPV-resistant apricot cultivars.

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References

  • Cambra M, Capote N, Myrta A, Llácer G (2006) Plum pox virus and the estimated costs associated with sharka disease. EPPO Bull 36:202–204

    Article  Google Scholar 

  • Decroocq V, Favé M, Hagen L, Bordenave L, Decroocq S (2003) Development and transferability of apricot and grape EST microsatellite markers across taxa. Theor Appl Genet 106:912–922

    PubMed  CAS  Google Scholar 

  • Dondini L, Lain O, Vendramin V, Rizzo M, Vivoli D, Adami M, Guidarelli M, Gaiotti F, Palmisano F, Bazzoni A, Boscia D, Geuna F, Tartarini S, Negri P, Castellano M, Savino V, Bassi D, Testolin R (2011) Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars. Mol Breed 27:289–299

    Article  Google Scholar 

  • Doyle J, Doyle J (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Ender M, Terpstra K, Kelly J (2008) Marker-assisted selection for white mold resistance in common bean. Mol Breed 21:149–157

    Article  CAS  Google Scholar 

  • Hospital F (2009) Challenges for effective marker-assisted selection in plants. Genetica 136:303–310

    Article  PubMed  Google Scholar 

  • Lalli D, Abbott A, Zhebentyayeva T, Badenes M-L, Damsteegt V, Polák J, Krška B, Salava J (2008) A genetic linkage map for an apricot (Prunus armeniaca L.) BC1 population mapping plum pox virus resistance. Tree Genet Genomes 4:481–493

    Article  Google Scholar 

  • Lambert P, Dicenta F, Rubio M, Audergon J-M (2007) QTL analysis of resistance to sharka disease in the apricot (Prunus armeniaca L.) 'Polonais' × 'Stark Early Orange' F1 progeny. Tree Genetics Genomes 6:291–304

    Google Scholar 

  • Lambert P, Karayiannis I, Poëssel J, Rubio M, Dicenta F, Audergon J-M, Decroocq V, Pascal T, Foulongne M, Schurdi-Levraud V, Restier V, Bachellez A (2009) Mapping the resistance to Plum pox virus (PPV) carried by the apricot 'Stark Early Orange' using different environmental conditions and genetic background; comparison with P. davidiana. Acta Hoticulturae 814:727–731

    Google Scholar 

  • Marandel G, Salava J, Abbott A, Candresse T, Decroocq V (2009) Quantitative trait loci meta-analysis of Plum pox virus resistance in apricot (Prunus armeniaca L.): new insights on the organization and the identification of genomic resistance factors. Mol Plant Pathol 10:347–360

    Article  PubMed  CAS  Google Scholar 

  • Palloix A, Ayme V, Moury B (2009) Durability of plant major resistance genes to pathogens depends on the genetic background, experimental evidence and consequences for breeding strategies. New Phytologist 183:190–199

    Article  PubMed  CAS  Google Scholar 

  • Pilařová P, Marandel G, Decroocq V, Salava J, Krška B, Abbott AG (2010) Quantitative trait analysis of resistance to Plum pox virus in the apricot F1 progeny ‘Harlayne’ × ‘Vestar’. Tree Genet Genomes 6:467–475

    Article  Google Scholar 

  • Richardson K, Vales M, Kling J, Mundt C, Hayes P (2006) Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theor Appl Genet 113:485–495

    Article  PubMed  CAS  Google Scholar 

  • Rubio M, Ruiz D, Egea J, Martínez-Gómez P, Dicenta F (2014) Opportunities of marker assisted selection for Plum pox virus resistance in apricot breeding programs. Tree Genetics Genomes. doi:10.1007/s11295-014-0700-x

    Google Scholar 

  • Salava J, Polák J, Krška B (2005) Oligogenic inheritance of resistance to Plum pox virus in apricot. Czech J Genet Plant Breed 41:167–170

    Google Scholar 

  • Salava J, Polák J, Krška B (2008) Two-gene resistance to Plum pox virus in apricot. Acta Horticult 781:303–307

    Google Scholar 

  • Sicard O, Loudet O, Keurentjes J-J-B, Candresse T, Le Gall O, Revers F, Decroocq V (2008) Identification of QTLs controlling symptom development during viral infection in Arabidopsis thaliana. Mol Plant-Microbe Interact 21:198–207

    Article  PubMed  CAS  Google Scholar 

  • Soriano J, Vera-Ruiz E, Vilanova S, Martínez-Calvo J, Llácer G, Badenes M-L, Romero C (2008) Identification and mapping of a locus conferring plum pox virus resistance in two apricot-improved linkage maps. Tree Genet Genomes 4:391–402

    Article  Google Scholar 

  • Soriano J, Domingo M, Zuriaga E, Romero C, Zhebentyayeva T, Abbott A, Badenes M-L (2012) Identification of simple sequence repeat markers tightly linked to plum pox virus resistance in apricot. Mol Breed 30:1017–1026

    Article  CAS  Google Scholar 

  • Vera Ruiz E, Soriano J-M, Romero C, Zhebentyayeva T, Terol J, Zuriaga E, Llacer G, Abbott AG, Badenes M-L (2011) Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region. Mol Plant Pathol 12:535–547

    Article  PubMed  Google Scholar 

  • Xu Y, Crouch J (2008) Marker-assisted selection in plant breeding: from publications to practice. Crop Sci 48:391–407

    Article  Google Scholar 

  • Zhebentyayeva TN, Reighard GL, Lalli DA, Gorina M, Krška B, Abbott AG (2008) Origin of resistance to plum pox virus in Apricot: what new AFLP and targeted SSR data analyses tell. Tree Genetics Genomes 4:403–417

    Article  Google Scholar 

  • Zuriaga E, Soriano JM, Zhebentyayeva T, Romero C, Dardick C, Cañizares J, Badenes M-L (2013) Genomic analysis reveals MATH gene(s) as candidate(s) for Plum pox virus (PPV) resistance in apricot (Prunus armeniaca L.). Mol Plant Pathol 14:663–677

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We acknowledge support of the EU in the frame of the FP7 KBBE-204429 SharCo project no 204429. The research of the authors is also supported by grants from the French interprofessional office for Fruits et Vegetables (FranceAgriMer, ex-Vinifhlor), the Ministry of Agriculture of the Czech Republic (no. MZE0002700604), and the Italian National Project PRIN 2006. We are grateful to S. Foschi (CRPV, Italy) and M. Lama (ASTRA, Italy) for maintaining the large number of plants used in this study and to C. Confolent (UGAFL, France) for participating in genotyping. Special thanks to Prof. A.G. Abbott (INRA, UMR BFP, France) and Dr. C. Dardick (AFRS-ARS, USA) for providing NGS sequences of PPV-resistant and susceptible accessions and Prof. A.G. Abbott for critical reading of the manuscript and for valuable comments. Molecular analysis of the Czech seedlings was performed at the Genomic and Sequencing Facility of Bordeaux (grants from the Conseil Regional d’Aquitaine no 20030304002FA and 20040305003FA and from the European Union, FEDER no 2003227 and from the Investissements d’avenir, ANR-10-EQPX-16-01).

Data Archiving Statement

Full sequence data (PCR fragments of Single-nucleotide polymorphism markers –SNPs-) will be submitted to GDR (http://www.rosaceae.org/). Next Generation sequences used here as well as apricot accessions resequenced correspond to the ones described in (and published by) Zuriaga et al. (2013).

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Authors and Affiliations

  1. UMR 1332 BFP, INRA-Université de Bordeaux, CS20032, 33882, Villenave d’Ornon, France

    S. Decroocq, A. Chague & V. Decroocq

  2. UR1052 GAFL, INRA, Domaine Saint Maurice, CS60094, 84143, Montfavet, France

    P. Lambert & J-M. Audergon

  3. CEP INNOVATION, INRA, 23 rue Jean Baldassini, 69364, Lyon cedex 7, France

    G. Roch

  4. Department of Agricultural and Environmental Sciences (DISAA), University of Milano, Via Celoria 2, 20133, Milan, Italy

    F. Geuna, R. Chiozzotto & D. Bassi

  5. Department of Agricultural Sciences (DipSA), University of Bologna, Viale Fanin 44, 40127, Bologna, Italy

    L. Dondini & S. Tartarini

  6. Division of Plant Health, Crop Research Institute, Drnovská 507, 161 06, Prague 6, Czech Republic

    J. Salava

  7. Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44, Lednice, Czech Republic

    B. Krška

  8. Istituto di Virologia Vegetale, CNR, UOS Bari, Via Amendola 165/A, 70126, Bari, Italy

    F. Palmisano

  9. Pomology Institute-National Agricultural Research Foundation (N.AG.RE.F.), R.R. Station 38, 592 00, Naoussa-Makedonia, Greece

    I. Karayiannis

Authors
  1. S. Decroocq
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  2. A. Chague
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  3. P. Lambert
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  4. G. Roch
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  5. J-M. Audergon
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  6. F. Geuna
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  7. R. Chiozzotto
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  8. D. Bassi
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  9. L. Dondini
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  10. S. Tartarini
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  11. J. Salava
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  12. B. Krška
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  13. F. Palmisano
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  14. I. Karayiannis
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  15. V. Decroocq
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Corresponding author

Correspondence to V. Decroocq.

Additional information

Communicated by A. M. Dandekar

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Table S1

R, resistant; S, susceptible; T, tolerant. Highlighted in gray, frequencies of PPV-susceptible (or tolerant) individuals genotyped as resistant. °: the number of PPV-susceptible individuals in this progeny is expected to be underestimated because while screening, the 177/nul (resistant) haplotype was undistinguishable from the 177/177, susceptible haplotype. *: frequency of the homozygous haplotype at this locus (XLSX 17.1 kb).

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Decroocq, S., Chague, A., Lambert, P. et al. Selecting with markers linked to the PPVres major QTL is not sufficient to predict resistance to Plum Pox Virus (PPV) in apricot. Tree Genetics & Genomes 10, 1161–1170 (2014). https://doi.org/10.1007/s11295-014-0750-0

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  • DOI: https://doi.org/10.1007/s11295-014-0750-0

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