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Virus Genes

, Volume 54, Issue 3, pp 470–473 | Cite as

Wuhan large pig roundworm virus identified in human feces in Brazil

  • Adriana Luchs
  • Elcio Leal
  • Shirley Vasconcelos Komninakis
  • Flavio Augusto de Pádua Milagres
  • Rafael Brustulin
  • Maria da Aparecida Rodrigues Teles
  • Danielle Elise Gill
  • Xutao Deng
  • Eric Delwart
  • Ester Cerdeira Sabino
  • Antonio Charlys da Costa
Article

Abstract

We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98–99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.

Keywords

Virus discovery Gastroenteritis Metagenomic Wuhan large pig roundworm virus Brazil 

Notes

Acknowledgements

We are grateful to Zymo Research Inc, Illumina Inc., Sage Science Inc., Promega Biotecnologia do Brasil Ltda, for the donation of reagents for this project.

Author contributions

AL and ACC conceived the study and designed the study protocol. FAPM, RB, and MART participated in the conduct of the study, collection, and screening of the specimens. SCVK, DG, ECS, and ACC performed the deep sequencing assays. EL, XD, ED, and ACC analyzed the big data. EL conducted the phylogenetic analysis. AL and ACC analyzed and interpreted the data. AL drafted the manuscript. All authors critically revised the manuscript for intellectual content and approved the final version. AL and ACC are guarantors of the paper.

Funding

Adriana Luchs is funded by FAPESP #2015/12944-9, and Antônio Charlys da Costa by FAPESP #2017/00021-9. This work was also supported by FAPESP #2016/01735-2 and CNPq #400354/2016-0.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Previous Ethics Committee approval was granted by Adolfo Lutz Institute, São Paulo, Brazil (CEP 965.723; CTC 45G-2014), Faculdade de Medicina da Universidade de São Paulo (CAAE: 53153916.7.0000.0065), and Centro Universitário Luterano de Palmas—ULBRA (CAAE 53153916.7.3007.5516). This was an anonymous unlinked study, and informed consent was not required according to resolution 466/12 concerning research involving humans (Conselho Nacional de Saúde/Ministério da Saúde, Brasília, 2012).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Adriana Luchs
    • 1
  • Elcio Leal
    • 2
  • Shirley Vasconcelos Komninakis
    • 3
    • 4
  • Flavio Augusto de Pádua Milagres
    • 5
    • 6
    • 7
  • Rafael Brustulin
    • 6
    • 7
  • Maria da Aparecida Rodrigues Teles
    • 7
  • Danielle Elise Gill
    • 8
  • Xutao Deng
    • 9
    • 10
  • Eric Delwart
    • 9
    • 10
  • Ester Cerdeira Sabino
    • 5
    • 8
  • Antonio Charlys da Costa
    • 8
  1. 1.Enteric Disease Laboratory, Virology CenterAdolfo Lutz InstituteSão PauloBrazil
  2. 2.Institute of Biological SciencesFederal University of ParáParáBrazil
  3. 3.Postgraduate Program in Health ScienceFaculty of Medicine of ABCSanto AndréBrazil
  4. 4.Retrovirology LaboratoryFederal University of São PauloSão PauloBrazil
  5. 5.LIM/46, Faculty of MedicineUniversity of São PauloSão PauloBrazil
  6. 6.Federal University of TocantinsPalmasBrazil
  7. 7.Public Health Laboratory of Tocantins State (LACEN/TO)PalmasBrazil
  8. 8.Institute of Tropical MedicineUniversity of São PauloSão PauloBrazil
  9. 9.Blood Systems Research InstituteSan FranciscoUSA
  10. 10.Department Laboratory MedicineUniversity of California San FranciscoSan FranciscoUSA

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