Skip to main content
SpringerLink
Log in

Wuhan large pig roundworm virus identified in human feces in Brazil

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98–99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. M. Shi, Y.Z. Zhang, E.C. Holmes, Meta-transcriptomics and the evolutionary biology of RNA viruses. Virus Res. 243, 83–90 (2018)

    Article  PubMed  CAS  Google Scholar 

  2. M. Shi, X.D. Lin, J.H. Tian, L.J. Chen, X. Chen, C.X. Li, X.C. Qin, J. Li, J.P. Cao, J.S. Eden, J. Buchmann, W. Wang, J. Xu, E.C. Holmes, Y.Z. Zhang, Redefining the invertebrate RNA virosphere. Nature 540, 539 (2016)

    Article  CAS  Google Scholar 

  3. L. Li, X. Deng, E.T. Mee, S. Collot-Teixeira, R. Anderson, S. Schepelmann, P.D. Minor, E. Delwart, Comparing viral metagenomics methods using a highly multiplexed human viral pathogens reagent. J. Virol. Methods 213, 139–146 (2015)

    Article  PubMed  CAS  Google Scholar 

  4. A.C. da Costa, J. Thézé, S.C.V. Komninakis, R.L. Sanz-Duro, M.R.L. Felinto, L.C.C. Moura, I.M.O. Barroso, L.E.C. Santos, M.A.L. Nunes, A.A. Moura, J. Lourenço, X. Deng, E.L. Delwart, M.R.D.A.S. Guimarães, O.G. Pybus, E.C. Sabino, N.R. Faria, Spread of chikungunya virus East/Central/South African genotype in Northeast Brazil. Emerg. Infect. Dis. 23(10), 1742–1744 (2017). https://doi.org/10.3201/eid2310.170307

    Article  Google Scholar 

  5. X. Deng, S.N. Naccache, T. Ng, S. Federman, L. Li, C.Y. Chiu, E.L. Delwart, An ensemble strategy that significantly improves de novo assembly of microbial genomes from metagenomic next-generation sequencing data. Nucleic Acids Res. 43(7), e46 (2015)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  6. A.J. Drummond, M.A. Suchard, D. Xie, A. Rambaut, Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol. Biol. Evol. 29(8), 1969–1973 (2012). https://doi.org/10.1093/molbev/mss075

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. S. Kumar, G. Stecher, K. Tamura, MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7), 1870–1874 (2016). https://doi.org/10.1093/molbev/msw054

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to Zymo Research Inc, Illumina Inc., Sage Science Inc., Promega Biotecnologia do Brasil Ltda, for the donation of reagents for this project.

Funding

Adriana Luchs is funded by FAPESP #2015/12944-9, and Antônio Charlys da Costa by FAPESP #2017/00021-9. This work was also supported by FAPESP #2016/01735-2 and CNPq #400354/2016-0.

Author information

Authors and Affiliations

  1. Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Av. Dr. Arnaldo, nº 355, São Paulo, SP, 01246-902, Brazil

    Adriana Luchs

  2. Institute of Biological Sciences, Federal University of Pará, Pará, Brazil

    Elcio Leal

  3. Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo André, Brazil

    Shirley Vasconcelos Komninakis

  4. Retrovirology Laboratory, Federal University of São Paulo, São Paulo, SP, Brazil

    Shirley Vasconcelos Komninakis

  5. LIM/46, Faculty of Medicine, University of São Paulo, São Paulo, Brazil

    Flavio Augusto de Pádua Milagres & Ester Cerdeira Sabino

  6. Federal University of Tocantins, Palmas, Tocantins, Brazil

    Flavio Augusto de Pádua Milagres & Rafael Brustulin

  7. Public Health Laboratory of Tocantins State (LACEN/TO), Palmas, Brazil

    Flavio Augusto de Pádua Milagres, Rafael Brustulin & Maria da Aparecida Rodrigues Teles

  8. Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, nº 470, São Paulo, SP, 05403-000, Brazil

    Danielle Elise Gill, Ester Cerdeira Sabino & Antonio Charlys da Costa

  9. Blood Systems Research Institute, San Francisco, USA

    Xutao Deng & Eric Delwart

  10. Department Laboratory Medicine, University of California San Francisco, San Francisco, USA

    Xutao Deng & Eric Delwart

Authors
  1. Adriana Luchs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elcio Leal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shirley Vasconcelos Komninakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Flavio Augusto de Pádua Milagres
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rafael Brustulin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maria da Aparecida Rodrigues Teles
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Danielle Elise Gill
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xutao Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Eric Delwart
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ester Cerdeira Sabino
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Antonio Charlys da Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

AL and ACC conceived the study and designed the study protocol. FAPM, RB, and MART participated in the conduct of the study, collection, and screening of the specimens. SCVK, DG, ECS, and ACC performed the deep sequencing assays. EL, XD, ED, and ACC analyzed the big data. EL conducted the phylogenetic analysis. AL and ACC analyzed and interpreted the data. AL drafted the manuscript. All authors critically revised the manuscript for intellectual content and approved the final version. AL and ACC are guarantors of the paper.

Corresponding authors

Correspondence to Adriana Luchs or Antonio Charlys da Costa.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Previous Ethics Committee approval was granted by Adolfo Lutz Institute, São Paulo, Brazil (CEP 965.723; CTC 45G-2014), Faculdade de Medicina da Universidade de São Paulo (CAAE: 53153916.7.0000.0065), and Centro Universitário Luterano de Palmas—ULBRA (CAAE 53153916.7.3007.5516). This was an anonymous unlinked study, and informed consent was not required according to resolution 466/12 concerning research involving humans (Conselho Nacional de Saúde/Ministério da Saúde, Brasília, 2012).

Additional information

Edited by William Dundon.

Accession number The complete bisegmented genome sequence of WLPRV/human/BRA/TO-34/2015 strain has been deposited in GenBank under accession numbers MG746618 (segment 1) and MG746619 (segment 2).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luchs, A., Leal, E., Komninakis, S.V. et al. Wuhan large pig roundworm virus identified in human feces in Brazil. Virus Genes 54, 470–473 (2018). https://doi.org/10.1007/s11262-018-1557-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-018-1557-0

Keywords

Search

Navigation