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In vitro induction of allohexaploid and resulting phenotypic variation in Populus

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Abstract

Although triploid Populus varieties have been used widely in timber and pulpwood production, the performance of economic traits in Populus with higher ploidy levels remains unknown due to a lack of germplasms with higher ploidy. In this study, we first successfully induced hexaploids in Populus by treating triploid leaf explants with colchicine in vitro. In total, 32 hexaploids were produced. The frequency of hexaploids was significantly affected by the interaction between colchicine concentration and exposure time. The highest hexaploid induction efficiency was 16.89% (± 2.26), which was achieved by treating explants with 0.04% colchicine for 7 days. Compared to triploids, hexaploids had thinner epidermal hair, larger stomata and protoplasts, and fewer chloroplasts, indicating that significant phenotypic changes accompanied an increase in ploidy level. These hexaploids are valuable for investigating the performance of economic traits in Populus with higher ploidy levels and have the potential to be used as parents to produce new tetraploid and pentaploid germplasms in Populus breeding programs.

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Abbreviations

MS:

Murashige and Skoog (1962) basal medium

IBA:

Indolebutyric acid

BA:

6-Benzyladenine

TDZ:

Thidiazuron (N-phenyl-N’-1,2,3-thiadiazol-5-ylurea)

MOPS:

4-Morpholinepropane sulfonate

PVP:

Polyvinylpyrrolidone

CPW:

Cell protoplast washing medium

MES:

4-Morpholine ethane sulfonic acid

BSA:

Bovine serum albumin

ANOVA:

Analysis of variance

LSD:

Least significant difference

SE:

Standard error

SEM:

Scanning electron microscopy

PI:

Propidium iodide

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31470662) and the program from the Beijing Municipal Education Commission (Grant No. CEFF-PXM2018_014207_000024). The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/D74xMK.

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Author notes

  1. Wanting Liu, Yunfei Zheng and Shaoyu Song are equally contributed to this work.

Authors and Affiliations

  1. National Engineering Laboratory in Tree Breeding, Beijing Forestry University, P.O. Box 118, Beijing, 100083, People’s Republic of China

    Wanting Liu, Yunfei Zheng, Shaoyu Song, Beibei Huo & Jun Wang

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Wanting Liu, Yunfei Zheng, Shaoyu Song, Beibei Huo & Jun Wang

  3. College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Wanting Liu, Yunfei Zheng, Shaoyu Song, Beibei Huo & Jun Wang

  4. Beijing Huang Fa Nursery, Beijing, 102601, People’s Republic of China

    Daili Li

Authors
  1. Wanting Liu
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  2. Yunfei Zheng
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  3. Shaoyu Song
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Contributions

JW conceived and designed research. WL, SS and BH conducted experiments. YZ and DL analyzed data and prepared figures. WL and JW wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Jun Wang.

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The authors declare that they have no competing interests.

Additional information

Communicated by Sergio J. Ochatt.

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Liu, W., Zheng, Y., Song, S. et al. In vitro induction of allohexaploid and resulting phenotypic variation in Populus. Plant Cell Tiss Organ Cult 134, 183–192 (2018). https://doi.org/10.1007/s11240-018-1411-z

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  • DOI: https://doi.org/10.1007/s11240-018-1411-z

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