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Genome-Wide Analysis of the ZRT, IRT-Like Protein (ZIP) Family and Their Responses to Metal Stress in Populus trichocarpa

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Abstract

The ZRT, IRT-like protein (ZIP) family plays an important role in the transport of zinc (Zn) and iron (Fe) across the cell membrane in many different species. However, studies on ZIP family are mainly limited in herbaceous species; hence, we investigated functional divergence of ZIP family in Populus trichocarpa. We identified 21 ZIP genes in P. trichocarpa and classified them into four groups based on phylogenetic analysis. Structural analyses revealed that most of the PtrZIP transporters have eight transmembrane domains (TMDs). PtrZIP members were unequally positioned in 19 P. trichocarpa linkage groups (LGs), with six tandem duplications and four segmental duplications. The promoter regions of PtrZIP genes contain Zn, Fe, copper (Cu), and other metal stress-related cis-elements. Additionally, tissue-specific expression of PtrZIP genes showed that most of them had relatively high expression levels in the root. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed that the expression of PtrZIP genes were induced not only under deficiency or excess condition of Zn, Fe, Cu and manganese (Mn) but also under excess condition of cadmium (Cd) and lead (Pb) stress. These findings indicated that PtrZIP genes may have played potential roles in metal transporters. Genome-wide analysis of PtrZIP genes in P. trichocarpa provided more comprehensive insights on the structure and function of this gene family.

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Acknowledgements

This work were supported by the National Natural Science Foundation of China (No. 31400573) and the 111 project (B16010).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang, 150040, China

    Haizhen Zhang, Dandan Li & Chenghao Li

  2. School of Pharmacy, Harbin University of Commerce, Harbin, 150076, China

    Shicheng Zhao

  3. Library of Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Xuemei Xu

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  1. Haizhen Zhang
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  2. Shicheng Zhao
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  4. Xuemei Xu
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  5. Chenghao Li
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Contributions

Haizhen Zhang, Shicheng Zhao, Xuemei Xu, and Chenghao Li designed the experiments and wrote the manuscript. Haizhen Zhang and Dandan Li performed the experiments. Haizhen Zhang, Shicheng Zhao, and Xuemei Xu analyzed the data.

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Correspondence to Chenghao Li.

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Data Archiving Statement

All identified amino acid of ZIP gene sequences were deposited into the NCBI database (http://www.ncbi.nlm.nih.gov/). The accession numbers are listed in Table 1.

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Zhang, H., Zhao, S., Li, D. et al. Genome-Wide Analysis of the ZRT, IRT-Like Protein (ZIP) Family and Their Responses to Metal Stress in Populus trichocarpa . Plant Mol Biol Rep 35, 534–549 (2017). https://doi.org/10.1007/s11105-017-1042-2

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