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Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean

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Abstract

Salinity together with waterlogging or flooding, a condition that occurs frequently in the field, can cause severe damage to crops. Combined flooding and salinity decreases the growth and survival of plants more than either stress alone. We report here the first proteomic analysis to investigate the global effects of saline flooding on multiple metabolic pathways. Soybean seedlings at the emergence (VE) stage were treated with 100 mM NaCl and flooded with water or 100 mM sodium chloride solution for 2 days. Proteins were extracted from hypocotyl and root samples and analyzed by two-dimensional gel electrophoresis followed by MALDI-TOF, MALDI-TOF/TOF mass spectrometry or immunoblotting. A total of 43 reproducibly resolved, differentially expressed protein spots visualized by Coomassie brilliant blue staining were identified by MALDI-TOF MS. Identities of several proteins were also validated by MS/MS analysis or immunoblot analysis. Twenty-nine proteins were upregulated, eight proteins were downregulated and six spots were newly induced. The identified proteins include well-known salt and flooding induced proteins as well as novel proteins expressed by the salinity-flooding combined stress. The comparative analysis identified changes at the proteome level that are both specific and part of a common or shared response. The identification of such differentially expressed proteins provides new targets for future studies that will allow assessment of their physiological roles and significance in the response of glycophytes to a combination of flooding and salinity.

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Acknowledgement

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-211-F00006). I Alam is supported by a post doctoral grant; SA Sharmin, KH Kim and YG Kim are supported by a scholarship from BK21 program at Gyeongsang National University, Republic of Korea.

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  1. Division of Applied Life Science (BK21 Program), IALS, PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea

    Iftekhar Alam, Shamima Akhtar Sharmin, Kyung-Hee Kim, Yong-Goo Kim, Jeong Dong Bahk & Byung-Hyun Lee

  2. Department of Applied Biology, Gyeongsang National University, Jinju, 660-701, Korea

    Jeung Joo Lee

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  1. Iftekhar Alam
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  2. Shamima Akhtar Sharmin
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Correspondence to Byung-Hyun Lee.

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Responsible Editor: John McPherson Cheeseman.

I. Alam and S.A. Sharmin contributed equally to this work.

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Supplementary Fig. 1

Effect of flooding (F), 100 mM NaCl (S) and saline flooding (SF) on the length and fresh weight of soybean hypocotyl and root. Fresh weight was calculated from the pooled weight of 12 seedlings (TIFF 2459 kb)

Supplementary Fig. 2

Enlarged views of CBB-stained gels of the differentially expressed proteins (indicated by arrows) marked in Fig. 3 (PDF 2571 kb)

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Alam, I., Sharmin, S.A., Kim, KH. et al. Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean. Plant Soil 346, 45–62 (2011). https://doi.org/10.1007/s11104-011-0792-0

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