Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean
- 603 Downloads
Salinity together with waterlogging or flooding, a condition that occurs frequently in the field, can cause severe damage to crops. Combined flooding and salinity decreases the growth and survival of plants more than either stress alone. We report here the first proteomic analysis to investigate the global effects of saline flooding on multiple metabolic pathways. Soybean seedlings at the emergence (VE) stage were treated with 100 mM NaCl and flooded with water or 100 mM sodium chloride solution for 2 days. Proteins were extracted from hypocotyl and root samples and analyzed by two-dimensional gel electrophoresis followed by MALDI-TOF, MALDI-TOF/TOF mass spectrometry or immunoblotting. A total of 43 reproducibly resolved, differentially expressed protein spots visualized by Coomassie brilliant blue staining were identified by MALDI-TOF MS. Identities of several proteins were also validated by MS/MS analysis or immunoblot analysis. Twenty-nine proteins were upregulated, eight proteins were downregulated and six spots were newly induced. The identified proteins include well-known salt and flooding induced proteins as well as novel proteins expressed by the salinity-flooding combined stress. The comparative analysis identified changes at the proteome level that are both specific and part of a common or shared response. The identification of such differentially expressed proteins provides new targets for future studies that will allow assessment of their physiological roles and significance in the response of glycophytes to a combination of flooding and salinity.
KeywordsAbiotic stress Combined stress Flooding Proteome Salinity Soybean
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-211-F00006). I Alam is supported by a post doctoral grant; SA Sharmin, KH Kim and YG Kim are supported by a scholarship from BK21 program at Gyeongsang National University, Republic of Korea.
- Barrett-Lennard E (1986) Effects of waterlogging on the growth and NaCl uptake by vascular plants under saline conditions. Reclamation Revegetation Res 5:245–261Google Scholar
- Khavari-Nejad RA, Bujar M, Attaran E (2006) Evaluation of anthocyanin contents under salinity (NaCl) stress in Bellis Perennis L. In Khan MA, Weber DJ (eds) Ecophysiology of high salinity tolerant plants, pp 127–134Google Scholar
- Meuriot F, Noquet C, Avice J-C, Volenec JJ, Cunningham SM, Sors TG, Caillot S, Ourry A (2004) Methyl jasmonate alters N partitioning, N reserves accumulation and induces gene expression of a 32-kDa vegetative storage protein that possesses chitinase activity in Medicago sativa taproots. Physiol Plant 120:113–123PubMedCrossRefGoogle Scholar
- Taylor NL, Howell KA, Heazlewood JL, Tan TYW, Narsai R, Huang S, Whelan J, Millar AH (2010) Analysis of the rice mitochondrial carrier family reveals anaerobic accumulation of a basic amino acid carrier involved in arginine metabolism during seed germination. Plant Physiol 154:691–704PubMedCrossRefGoogle Scholar
- Zubini P, Zambelli B, Musiani F, Ciurli S, Bertolini P, Baraldi E (2009) The RNA hydrolysis and the cytokinin binding activities of PR-10 proteins are differently performed by two isoforms of the Pru p 1 peach major allergen and are possibly functionally related. Plant Physiol 150:1235–1247PubMedCrossRefGoogle Scholar