Plant Molecular Biology

, Volume 80, Issue 2, pp 237–240 | Cite as

Arabidopsis light-dependent NADPH: protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development: an addendum

  • Chanhong Kim
  • Klaus Apel
Brief Communication


Recently the porA-1 null mutant of Arabidopsis thaliana has been identified, which contains an insertion of the Dissociation (Ds) element in the PORA gene (Paddock et al. in Plant Mol Biol 78:447–460, 2012). Light-grown porA-1 seedlings suffer from a drastically reduced chlorophyll content and a developmental arrest beyond the cotyledon stage, suggesting that PORA is not only transiently involved in initiating chlorophyll synthesis during illumination of etiolated seedlings but is also essential for normal growth and plant development. Here we report the presence of a second Ds element in this porA-1 mutant line that inactivates the Speechless gene required for stomata formation. Similar to porA-1, speechless seedlings are severely impaired in their development. Our results suggest that the lack of stomata in porA-1 may contribute to the dwarfed phenotype of the mutant and thus emphasizes the need to re-address the proposed role of PORA during plant development by studying a porA mutant that retains its stomata formation.


NADPH: protochlophyllide oxidoreductase A Speechless Seedling development Chlorophyll synthesis 



We thank Dr. Troy Paddock for sending us seeds of the porA-1 mutant and for helpful discussions. This study was supported by the Boyce Thompson Institute for Plant Research.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Boyce Thompson Institute for Plant ResearchIthacaUSA

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