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Leptolyngbya CCM 4, a cyanobacterium with far-red photoacclimation from Cuatro Ciénegas Basin, México

  • Published:
Photosynthetica

Abstract

A cyanobacterium containing phycobiliproteins with far-red acclimation was isolated from Pozas Rojas, Cuatro Ciénegas, México. It was named Leptolyngbya CCM 4 after phylogenetic analysis and a description of its morphological characteristics. Leptolyngbya was grown in far-red light. Sucrose-gradient analysis of the pigments revealed two different colored bands of phycobiliproteins. A band at 60% sucrose was a phycocyanin containing phycobilisome; at 35% sucrose, a new type of phycobiliprotein absorbed at 710 nm. SDS-PAGE revealed the presence of two types of core-membrane linkers. Analysis of the hydrophobic pigments extracted from the thylakoid membranes revealed Chl a, d, and f. The ratio of Chl f/a was reversibly changed from 1:12–16 under far-red light to an undetectable concentration of Chl f under white light. Cuatro Ciénegas, a place surrounded by the desert, is a new ecosystem where a cyanobacterium, which grows in farred light, was discovered.

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Abbreviations

AP:

allophycocyanin

Car:

carboxisomes

CCA:

complementary chromatic acclimation

CNE:

clear native electrophoresis

FaRLiP:

far-red light photoacclimation

FNR:

ferredoxin-NADP+ reductase

Fr.:

fractions

LCM :

core membrane linker

PBP:

phycobiliprotein

PBS:

phycobilisome

PC:

phycocyanin

PMSF:

phenylmethylsulfonyl fluoride

REP:

repetitive sequence

RL:

red light

Th:

thylakoid membranes

WCE:

whole cell extract

WL:

white light

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Authors and Affiliations

  1. Departmento de Bioquímica, Centro de Investigación y Estudios Avanzados del IPN, CdMx, Mexico

    C. Gómez-Lojero, L. E. Leyva-Castillo, P. Herrera-Salgado & J. Barrera-Rojas

  2. Laboratorio Nacional de Servicios Experimentales Centro de Investigación y Estudios Avanzados del IPN, Tlanepantla, Mexico

    E. Ríos-Castro

  3. Unidad de Biomedicina, FES-Iztacala UNAM, Tlanepantla, Mexico

    E. B. Gutiérrez-Cirlos

Authors
  1. C. Gómez-Lojero
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  2. L. E. Leyva-Castillo
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  3. P. Herrera-Salgado
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  4. J. Barrera-Rojas
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  5. E. Ríos-Castro
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  6. E. B. Gutiérrez-Cirlos
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Corresponding author

Correspondence to C. Gómez-Lojero.

Additional information

Acknowledgments: This paper is dedicated to the memory of Dr. David W Krogmann: professor, colleague, and friend. CGL thanks Dr. Valeria Souza for the opportunity to be at Cuatro Ciénegas in May, 2012, where the mat sample was obtained. The authors wish to thank to Mr. Jorge Zarco Mendoza for his technical expertise, Dr. Laura Ongay and Biologist Ma. Guadalupe Codiz of the Unidad de Biología Molecular, Instituto de Fisiología Celular UNAM for DNA sequencing, and Mrs. Leticia Gómez-Sandoval for her secretarial assistance.

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Gómez-Lojero, C., Leyva-Castillo, L.E., Herrera-Salgado, P. et al. Leptolyngbya CCM 4, a cyanobacterium with far-red photoacclimation from Cuatro Ciénegas Basin, México. Photosynthetica 56, 342–353 (2018). https://doi.org/10.1007/s11099-018-0774-z

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  • DOI: https://doi.org/10.1007/s11099-018-0774-z

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