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Influence of the disaccharide trehalose on the oxidizing side of photosystem II

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Photosynthetica

Abstract

The steady-state oxygen evolution rate was previously shown to be stimulated by the disaccharide trehalose in PSII suspension. Here we showed a similar increase in the rate of oxygen evolution in PSII core complexes from spinach in solution and in proteoliposomes in the presence of trehalose. Using direct electrometrical technique, we also revealed that trehalose had no effect on the kinetics of electron transfer from Mn to redox-active-tyrosyl radical, YZ• (S1 → S2 transition), while it accelerated the kinetics of electrogenic proton transport during S2 → S3 and S4 → S0 transitions of the wateroxidizing complex (WOC) induced by the first, second, and third laser flashes in dark-adapted PSII samples. These observations imply that the effect of trehalose occurrs due to its interaction with the WOC.

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Abbreviations

Chl:

chlorophyll

P680 :

the primary electron donor of PSII

QA, QB :

the primary and the secondary plastoquinone electron acceptors of PSII, respectively

RC:

reaction center

S0-S4 :

charge storage states of the WOC

WOC:

water-oxidizing complex

YZ :

redox active tyrosine residue 161 of D1 protein

Δψ :

transmembrane electric potential difference

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Authors and Affiliations

  1. Belozersky Institute of Physical-Chemical Biology, Lomonosov Moscow State University, GSP-1, Leninskie Gory, Moscow, 119991, Russia

    M. D. Mamedov, L. A. Vitukhnovskaya, A. A. Zaspa & A. Yu. Semenov

  2. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia

    E. S. Nosikova

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  1. M. D. Mamedov
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  2. E. S. Nosikova
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  3. L. A. Vitukhnovskaya
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  4. A. A. Zaspa
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  5. A. Yu. Semenov
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Correspondence to M. D. Mamedov.

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Acknowledgment: This work has the support from the Russian Science Foundation (Grant 17-14-01323).

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Mamedov, M.D., Nosikova, E.S., Vitukhnovskaya, L.A. et al. Influence of the disaccharide trehalose on the oxidizing side of photosystem II. Photosynthetica 56, 236–243 (2018). https://doi.org/10.1007/s11099-017-0750-z

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