Abstract
Ca2+ is an important factor mediating many biotic and abiotic stress responses in plants. In this study, we measured the chlorophyll (Chl) fluorescence of transgenic rice with increased or decreased expression of a calcium-sensing receptor (OsCaS) gene during water deficit caused by polyethylene glycol to prove our hypothesis that increased Ca2+ in combination with increased OsCaS could enhance the drought resistance of transgenic rice. Transcript abundance (evaluated by RT-PCR) was significantly lower in OsCaS antisense line 766 (AS766) than that in the wild type, while the overexpression line 777 (O777) showed four times higher amount than that in the wild type. Chl fluorescence showed that the photochemical quantum yield of PSII in the light increased due to addition of Ca2+ in the O777, but dropped in the AS766. Nonphotochemical quenching increased under stress in both transgenic lines and in the wild type, but less in the O777. Nonregulatory quantum yield of energy dissipation showed no significant change under drought stress. Photochemical quenching was significantly higher in the O777 than those in the AS766 and in the wild type after the Ca2+ treatment. In the absence of stress, the electron transport rate (ETR) was significantly higher in the O777 than in both the AS766 and the wild type. In contrast, the ETR of the wild type and both transgenic lines decreased under drought stress, while the effect of polyethylene glycol was partially alleviated by Ca2+ addition in the O777. In summary, excitation energy conversion and dissipation by PSII were regulated by Ca2+ in the O777. It might partially alleviate the effect of drought stress, whereas addition of Ca2+ had no effect in the wild type and the AS766.
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Abbreviations
- AS766:
-
OsCaS antisense line 766
- CaS:
-
calcium-sensing receptor
- [Ca2+]:
-
concentration of Ca2+
- [Ca2+]i :
-
concentration of cytosolic Ca2+
- [Ca2+]o :
-
concentration of extracellular Ca2+
- ETR:
-
electron transport rate
- O777OsCaS :
-
overexpression line 777
- PEG:
-
polyethylene glycol
- qP :
-
photochemical quenching
- WT:
-
wild type
- YNO :
-
nonregulatory quantum yield of energy dissipation
- YNPQ :
-
nonphotochemical quenching
- YPSII :
-
practical quantum yield of PSII
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Acknowledgements: We thank to Dr. Yikun He of Capital Normal University for helpful discussion, to Dr. Tiegang Lu of Chinese Academy of Agricultural Sciences for donating pCUbi1390 vector, and to Dr. Yaping Fu and Wenzhen Liu of China National Rice Research Institute for help with transformation of rice and planting the experimental materials. We thank to Dr. Michael Deyholos for help with amending the article. This work was supported by National Nature Science Foundation of China (30900771) and Beijing Natural Science Foundation (5102006).
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Wei, R., Liu, Y., Sui, Y. et al. Effects of Ca2+ and polyethylene glycol on the chlorophyll fluorescence parameters of transgenic OsCaS rice (Oryza sativa L.). Photosynthetica 53, 336–341 (2015). https://doi.org/10.1007/s11099-015-0107-4
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DOI: https://doi.org/10.1007/s11099-015-0107-4