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Photosynthetica

, Volume 51, Issue 3, pp 387–394 | Cite as

Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis

  • X. -G. Li
  • J. Yang
  • R. Wang
  • X. -F. Tang
  • J. -J. Meng
  • H. -J. Qin
  • X. Liu
  • F. Guo
  • S. -B. Wan
Article

Abstract

Pyrococcus furiosus is a hyperthermophilic archaeon. Its ribulose-1,5-bisphosphate carboxylase/oxygenase (PfRubisco) has only large subunit (L). PfRubisco has a novel (L2)5, decameric structure and it possesses higher carboxylase activity and thermotolerance. To assess the potential functionality of PfRubisco in higher plants under high-temperature stress, PfRubisco coding sequence was transiently expressed in Nicotiana benthamiana by Pea early browning virus mediated ectopic expression. The transgenic PfRubisco plants produced chlorotic yellow stripes in their leaves. Relative to the control leaves, those with yellow stripes exhibited decreased net photosynthetic rate and chlorophyll content, altered chloroplast ultrastructure, and more severe photoinhibition of both photosystem I and II. We concluded that the ectopic expression of PfRubisco might disrupt the chloroplast development and function in N. benthamiana. The potential cause of the disruption was discussed.

Additional key words

high-temperature stress Nicotiana benthamiana photosynthesis Rubisco virus mediated ectopic expression 

Abbreviations

Chl

chlorophyll

Ci

intercellular CO2 concentration

CK

noninoculated plants as a control

Fm

maximum yield of chlorophyll fluorescence in dark adapted state

Fv

variable fluorescence

Fv/Fm

maximal photochemical efficiency of PSII

GFP

green fluorescent protein

gs

stomatal conductance

L

large subunit

NbTp

Nicotiana benthamiana ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit transit peptide gene

NPQ

nonphotochemical quenching

PN

net photosynthetic rate

PS

photosystem

qRT-PCR

quantitative reverse transcription-polymerase chain reaction

rbcL

large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase

rbcS

small subunit of Rubisco

RT-PCR

reverse transcription-polymerase chain reaction

Rubisco

ribulose-1,5-bisphosphate carboxylase/oxygenase

RuBP

ribulose-1,5-bisphosphate

S

small subunit

T

inoculated, transgenic PfRubisco plants

VMEE

virus mediated ectopic expression

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • X. -G. Li
    • 1
  • J. Yang
    • 2
  • R. Wang
    • 2
  • X. -F. Tang
    • 3
  • J. -J. Meng
    • 1
  • H. -J. Qin
    • 4
  • X. Liu
    • 4
  • F. Guo
    • 1
  • S. -B. Wan
    • 1
  1. 1.High-Tech Research Center, Shandong Academy of Agricultural Sciences and Shandong Provincial Key Laboratory of Genetic ImprovementEcology and Physiology of CropsJi’nanChina
  2. 2.Zhengzhou Tobacco Research Institute of ChinaNational Tobacco CorporationZhengzhouChina
  3. 3.College of Life SciencesWuhan UniversityWuhanChina
  4. 4.The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

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