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Interactive effects of temperature and light intensity on photosynthesis and antioxidant enzyme activity in Zizania latifolia Turcz. plants

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Photosynthetica

Abstract

We compared the interactive effects of temperature and light intensity on growth, photosynthetic performance, and antioxidant enzyme activity in Zizania latifolia Turcz. plants in this study. Plants were grown under field (average air temperature 9.6–25°C and average light intensity 177–375 W m−2) or greenhouse (20–32°C and 106–225 W m−2) conditions from the spring to the early summer. The results indicated that greenhouse-grown plants (GGP) had significantly higher plant height, leaf length, and leaf width, but lower leaf thickness and total shoot mass per cluster compared with field-grown plants (FGP). Tiller emergence was almost completely suppressed in GGP. Significantly higher chlorophyll (Chl) content and lower Chl a/b ratio were observed in GGP than in FGP. From 4 to 8 weeks after treatment (WAT), net photosynthetic rate (P N) was significantly lower in FGP than in GGP. However, from 9 to 12 WAT, P N was lower in GGP, accompanied by a decrease in stomatal conductance (g s) and electron transport rate (ETR) compared with FGP. Suppressed P N in GGP under high temperature combined with low light was also indicated by photosynthetic photon flux density (PPFD) response curve and its diurnal fluctuation 10 WAT. Meanwhile, ETR in GGP was also lower than in FGP according to the ETR — photosynthetically active radiation (PAR) curve. The results also revealed that GGP had a lower light saturation point (LSP) and a higher light compensation point (LCP). From 4 to 8 WAT, effective quantum yield of PSII photochemistry (ΦPSII), photochemical quenching (qP), and ETR were slightly lower in FGP than in GGP. The activities of ascorbate peroxidase (APX), guaiacol peroxidase (POD), glutathione reductase (GR), superoxide dismutase (SOD), and malondialdehyde (MDA) content were significantly higher from 4 to 8 WAT, but lower from 10 to 12 WAT in FGP. However, catalase (CAT) activity was significantly lower in FGP from 4 to 8 WAT. Our results indicated that the growth and photosynthetic performance of Z. latifolia plants were substantially influenced by temperature, as well as light intensity. This is helpful to understand the physiological basis for a protected cultivation of this crop.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbic acid

C i :

intercellular CO2 concentration

CAT:

catalase

Chl:

chlorophyll

DM:

dry mass

E :

transpiration rate

ETR:

electron transport rate

FGP:

field-grown plants

FM:

fresh mass

F0 :

minimal fluorescence yield of the dark-adapted state

F0′:

minimal fluorescence yield of the light-adapted state

Fm :

maximal fluorescence yield of the dark-adapted state

Fm′:

maximal fluorescence yield of the light-adapted state

Fs :

steady-state fluorescence yield

Fv :

variable fluorescence

Fv/Fm :

maximal quantum yield of PSII photochemistry

GGP:

greenhouse-grown plants

GR:

glutathione reductase

g s :

stomatal conductance

LCP:

light-compensation point

LSP:

light-saturation point

MDA:

malondialdehyde

NPQ:

nonphotochemical quenching

PAR:

photosynthetically active radiation

P Nmax :

light-saturated net photosynthetic rate

P N :

net photosynthetic rate

POD:

guaiacol peroxidase

PPFD:

photosynthetic photon flux density

PSII:

photosystem II

qP :

photochemical quenching

ROS:

reactive oxygen species

SOD:

superoxide dismutase

WAT:

weeks after treatment

ΦPSII :

effective quantum yield of PSII photochemistry

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Authors and Affiliations

  1. Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

    N. Yan, X. -F. Xu, Z. -D. Wang & D. -P. Guo

  2. Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, P.R. China

    J. -Z. Huang

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  1. N. Yan
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  2. X. -F. Xu
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  3. Z. -D. Wang
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  4. J. -Z. Huang
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  5. D. -P. Guo
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Correspondence to D. -P. Guo.

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Acknowledgements: This research was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (No: 200903017-03).

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Yan, N., Xu, X.F., Wang, Z.D. et al. Interactive effects of temperature and light intensity on photosynthesis and antioxidant enzyme activity in Zizania latifolia Turcz. plants. Photosynthetica 51, 127–138 (2013). https://doi.org/10.1007/s11099-013-0009-2

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  • DOI: https://doi.org/10.1007/s11099-013-0009-2

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