Abstract
Tuberculosis (TB) is a disease of global importance. There is an increasing recognition of the role of Toll like receptors, important pattern recognition receptors of host immune system, in determining the susceptibility or resistance to TB in various populations. In an attempt to examine the importance of Toll like receptors in immune response to Mycobacterium tuberculosis infection, we explored two variants each of TLR2 and TLR9 in a population residing in Uttar Pradesh, India. Genotyping was performed to detect -196 to -174 del polymorphism and G2258A SNP (Arg753Gln, rs5743708) in TLR2 gene and -T1237C (rs5743836) and G2848A (rs352140) SNP in TLR9 gene in patients with pulmonary TB and healthy controls. The A allele of G2848A SNP in TLR9 gene was found with a marginally higher frequency among TB patients as compared to healthy controls, suggesting that A allele at position 2848 of TLR9 gene may be associated with susceptibility to TB in North Indian population [p = 0.05, Mantel–Haenszel OR = 1.34, 95% CI (1.0–1.82)].
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Acknowledgements
We thank Dr. Lutz Hamann (Institute for Microbiology and Hygiene, Charite-University Medicine Berlin), for his kind help in the designing of primers and probes for TLR9 G2848A SNP. We also thank the technical staff of Immunology and Microbiology lab for their help. Our sincere thanks to Dr Subramanyam Vemulpad (Faculty of Science and Engineering, Macquarie University, Sydney NSW 2109) for editing the manuscript. We thank Dr Ekata Sinha for useful discussion during manuscript revision.
Funding
This work was supported by the Department of Science and Technology, Government of India through SERC programme [Grant No. DST No: SR/SO/HS-54/2003]. Part of the funding was obtained from Institute Intramural funding supported by Indian Council of Medical Research. JRF (NET) and SRF (NET) of Mayank Mittal was funded by Council of Scientific and Industrial Research, Government of India [Grant No. 09/830(0008)/2006-EMR-I].
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All procedures involving human participants performed in this study were in accordance with the ethical standards of the Institute Human Ethics committee which was constituted as per the guidelines formulated by Indian Council of Medical Research [22].
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Informed consent was obtained from all individual participants included in this study.
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Mittal, M., Biswas, S.K., Singh, V. et al. Association of Toll like receptor 2 and 9 gene variants with pulmonary tuberculosis: exploration in a northern Indian population. Mol Biol Rep 45, 469–476 (2018). https://doi.org/10.1007/s11033-018-4182-z
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DOI: https://doi.org/10.1007/s11033-018-4182-z