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Using stem cell oxygen physiology to optimize blastocyst culture while minimizing hypoxic stress

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Abstract

This review is a response to the Fellows Forum on testing 2% oxygen for best culture of human blastocysts (J Ass Reprod Gen 34:303–8, 1; J Ass Reprod Gen 34:309–14, 2) prior to embryo transfer. It is a general analysis in support of the position that an understanding of stem cell physiology and responses to oxygen are necessary for optimization of blastocyst culture in IVF and to enhance reproductive success in fertile women.

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Acknowledgements

Thanks to Dr. Quanwen Li, Dr. Ali Faqi, Dr. Shu Takayama, and members of our lab for the analysis and comments on the manuscript. This research was supported by grants to DAR from NIH (1R03HD061431 02), the Kam Moghissi chair (EEP), and from the Office of the Vice President for Research at Wayne State University.

Author information

Author notes

  1. Alan Bolnick

    Present address: Department of Obstetrics and Gynecology, Kaleida Women’s and Children’s Hospital Buffalo New York, Buffalo, NY, USA

  2. Yufen Xie

    Present address: Fertility and Surgical Associates of California, Thousand Oaks, CA, 91361, USA

  3. Sichang Zhou

    Present address: University of Michigan Medical School, Ann Arbor, MI, 48109, USA

Authors and Affiliations

  1. CS Mott Center for Human Growth and Development, Department of Ob/Gyn, Reproductive Endocrinology and Infertility, Wayne State University School of Medicine, Detroit, MI, 48201, USA

    Alan Bolnick, Awoniyi O Awonuga, Yu Yang, Mohammed Abdulhasan, Elizabeth E. Puscheck & Daniel A. Rappolee

  2. Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA

    Yu Yang & Daniel A. Rappolee

  3. Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA

    Daniel A. Rappolee

  4. Institutes for Environmental Health Science, Wayne State University School of Medicine, Detroit, MI, USA

    Daniel A. Rappolee

  5. Department of Biology, University of Windsor, Windsor, ON, N9B 3P4, Canada

    Daniel A. Rappolee

  6. CS Mott Center for Human Growth and Development, Wayne State University School of Medicine, 275 East Hancock, Detroit, MI, 48201, USA

    Daniel A. Rappolee

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  1. Alan Bolnick
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Bolnick, A., Awonuga, A., Yang, Y. et al. Using stem cell oxygen physiology to optimize blastocyst culture while minimizing hypoxic stress. J Assist Reprod Genet 34, 1251–1259 (2017). https://doi.org/10.1007/s10815-017-0971-x

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