Effect of biodegradable chelating ligands on Fe uptake in and growth of marine microalgae

  • Hiroshi Hasegawa
  • Ayumi Nozawa
  • Rimana Islam Papry
  • Teruya Maki
  • Osamu Miki
  • M. Azizur Rahman
Article

Abstract

Iron (Fe) is an important nutrient for phytoplankton. The low solubility of Fe in oxic waters can be a growth-limiting factor for phytoplankton. Synthetic aminopolycarboxylates (APCs) such as ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) are widely used as Fe complexing agents for microalgae culture. However, the presence of these non-ready biodegradable APCS in aquatic systems may have serious environmental consequences. In the present study, the effects of biodegradable chelating ligands (hydroxyiminodisuccinic acid (HIDS), methylglycinediacetic acid (MGDA), and iminodisuccinate (IDS)) on Fe uptake in and growth of three coastal microalgae (Heterosigma akashiwo, Prymnesium parvum, and Skeletonema marinoi-dohrnii complex) were investigated, and the results were compared with those of non-ready biodegradable APCs (EDTA, ethylenediamine tetra-methylene phosphonic acid (EDTMP), and DTPA). The biodegradable chelating ligands did not have significant growth inhibition effect on the phytoplankton. Although the growth of the algae (except S. marinoi-dohrnii complex) was not affected substantially by 1.5 and 7.5 μM of DTPA, growth inhibition occurred by 7.5 μM of EDTMP and 150 μM of EDTA, DTPA, and EDTMP. The effect of chelating ligands on microalgal growth was likely to be associated with the intracellular Fe uptake influenced by the chelating ligands. On average, intracellular Fe concentrations for biodegradable chelating ligands were substantially higher than those for non-ready biodegradable APCs. Except H. akashiwo, the ratio of intra/extracellular Fe concentrations was highest for MGDA followed by IDS and HIDS. The results indicate that biodegradable chelating ligands are more efficient than non-ready biodegradable APCs in intracellular Fe uptake and algal growth.

Keywords

Microalgae Growth Fe uptake APCs Chelating ligands 

Notes

Acknowledgements

We would like to thank Dr. Christel Hassler, University of Geneva, Switzerland, for reviewing the manuscript and making a number of helpful suggestions prior to submission.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Science and EngineeringKanazawa UniversityKanazawaJapan
  2. 2.Graduate School of Natural Science and TechnologyKanazawa UniversityKanazawaJapan
  3. 3.Faculty of Science and TechnologyFederation UniversityGippsland, ChurchillAustralia

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