Autophagy regulates death of retinal pigment epithelium cells in age-related macular degeneration
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Age-related macular degeneration (AMD) is an eye disease underlined by the degradation of retinal pigment epithelium (RPE) cells, photoreceptors, and choriocapillares, but the exact mechanism of cell death in AMD is not completely clear. This mechanism is important for prevention of and therapeutic intervention in AMD, which is a hardly curable disease. Present reports suggest that both apoptosis and pyroptosis (cell death dependent on caspase-1) as well as necroptosis (regulated necrosis dependent on the proteins RIPK3 and MLKL, caspase-independent) can be involved in the AMD-related death of RPE cells. Autophagy, a cellular clearing system, plays an important role in AMD pathogenesis, and this role is closely associated with the activation of the NLRP3 inflammasome, a central event for advanced AMD. Autophagy can play a role in apoptosis, pyroptosis, and necroptosis, but its contribution to AMD-specific cell death is not completely clear. Autophagy can be involved in the regulation of proteins important for cellular antioxidative defense, including Nrf2, which can interact with p62/SQSTM, a protein essential for autophagy. As oxidative stress is implicated in AMD pathogenesis, autophagy can contribute to this disease by deregulation of cellular defense against the stress. However, these and other interactions do not explain the mechanisms of RPE cell death in AMD. In this review, we present basic mechanisms of autophagy and its involvement in AMD pathogenesis and try to show a regulatory role of autophagy in RPE cell death. This can result in considering the genes and proteins of autophagy as molecular targets in AMD prevention and therapy.
KeywordsAge-related macular degeneration Cell death Apoptosis Necroptosis Pyroptosis Alu transcripts
Age-related macular degeneration
Age-related maculopathy ancillary
Calcium-binding and coiled-coil domain 2/nuclear dot protein, 52 kDa
Alpha-crystallin A chain
DNA damage response
Death-inducing signaling complex
Genome-wide association study
Kelch-like ECH-associated protein 1
Microtubule-associated protein light chain 3
Nuclear factor (erythroid-derived 2)-like 2
Poly(ADP-ribose) polymerase 1
Polyunsaturated fatty acid
Retinal pigment epithelium
TNF receptor-associated factor 6
Various cell death modes are considered to play a role in AMD, including apoptosis, pyroptosis (cell death dependent on caspase-1), and necroptosis (regulated necrosis dependent on the proteins RIPK3 and MLKL, independent of caspases). However, there are some conflicting reports on the regulation of the choice of a particular cell death type in AMD, but surely, cells do not simply “choose” a certain death pathway, as all different pathways influence each other in a molecular crosstalk. Aberrant autophagy has been mentioned frequently to associate with AMD, and autophagy itself is considered a kind of programmed cell death, so it can play a role in cell death in AMD (Shimizu et al. 2010) (reviewed in Tsujimoto and Shimizu (2005)). In addition, apoptotic signaling can directly regulate autophagy and vice versa (reviewed in Yonekawa and Thorburn (2013)). In general, aberrant regulation of programmed cell death by autophagy is linked with many human diseases (reviewed in Mizumura et al. (2014a)) (Mizumura et al. 2014b). Therefore, it seems reasonable and justified to study the role of autophagy in the regulation of RPE cell death in AMD.
Age-related macular degeneration—an eye disease involving extensive cell death
Age-related macular degeneration is an eye disease affecting mainly elderly people, and it is a major cause of blindness among individuals over 65 years in developed countries (Reibaldi et al. 2016). As mentioned, two basic forms of advanced AMD are dry (non-exudative) and wet (exudative), but early dry AMD is hardly detectable, and its advanced form, called geographic atrophy (GA), which is associated with a massive loss of retinal cells, is responsible for a substantial part of blindness related to AMD (Klein et al. 2007).
AMD is a complex disease with genetic and environmental factors involved in its etiology. Although the exact mechanism of its pathogenesis is not known, oxidative stress and inflammation can play a role (reviewed in Kauppinen et al. (2016)). However, it is not exactly known how oxidative stress can contribute to the degeneration of RPE cells, and two mechanisms of their death, apoptosis and necrosis, are considered and supported by both in vivo and in vitro studies. Apoptosis is frequently attributed to RPE cell death in AMD, and both the caspase-dependent and -independent pathways are suggested to be involved (reviewed in Adler et al. (1999), Ferrington et al. (2016), and Hanus et al. (2015a)) (Dunaief et al. 2002; Hanus et al. 2015b; Rodrigues et al. 2011; Tsao et al. 2006; Wang et al. 2012). Hanus et al. (2013) suggested that necrosis, and not apoptosis, is a dominant cell death in RPE cells subjected to oxidative stress. In that study, the contribution of autophagy to RPE cell death was not observed. We also noted a dominancy of necrotic cell death in ARPE-19 cells treated with tert-butyl hydroxyperoxide (Tokarz et al. 2016). However, the problem of the involvement of necrosis in RPE cell death is still controversial and needs further studies.
Several issues should be taken into account in considering mechanisms of cellular death in AMD, among them two seem to be especially important. Firstly, AMD is a disease, which phenotype can dramatically change with its progression. Secondly, the region of the retina affected in AMD contains a highly heterogeneous population of cells, including RPE cells, choriocapillares, and neurons. The survival of neurons in the retina is regulated in a much more complex way than that of RPE cells, as these post-mitotic cells must function within neuronal circuits in the organism (reviewed in Kole et al. (2013)). Many factors can contribute to survival and death of retinal cells, but some of them can act differently in different cells, depending on their receptors (reviewed in Adler et al. (1999)). This mechanism is even more complicated by the concept that AMD can be associated with disturbed cell communication (reviewed in Nakahara et al. (2013)). This effect can result in a receiving of a death signal by one type of cells and transmitting it, in a changed form, to other types. Alternatively, cells receiving this primary signal can change the local environment, which affects other cells leading to their degeneration. Recruitment of inflammatory cells, changed cellular phenotypes, and autocrine and paracrine signaling alterations certainly modify the local tissue environment that may finally lead to cell death.
Autophagy—at the crossroad between cell death and survival
The involvement of autophagy in removing faulty cellular components makes it as an internal quality control mechanism in the cell. This has led to the concepts of general autophagy called non-selective autophagy and selective autophagy—the latter is directed to selective degradation of specific cellular components, first of all proteins or invading pathogens (reviewed in Mizushima and Komatsu (2011)). It is anticipated that even 1.5% of all cellular proteins in rat liver can be degraded by autophagy per hour in normal nutrition (Ezaki et al. 2011) (reviewed in Ueno et al. (2012)). Selective autophagy plays an important role in tissue differentiation, contributing to different transfer of genetic information in different cells. One of the most significant substrates for selective autophagy is the p62 protein (p62/SQSTM1), interacting with microtubule-associated protein light chain 3 (LC3), facilitating degradation of ubiquitinated substrates with its own subsequent degradation in autophagosome (reviewed in Johansen and Lamark (2011)). Therefore, p62 can be considered a marker of autophagic flux (Johansson et al. 2015; Viiri et al. 2013). The accumulation of p62 is associated with disturbed autophagy and observed in serious diseases including brain and liver cancers, Parkinson disease, Alzheimer disease, and steatohepatitis (Zatloukal et al. 2002). p62 decides the fate of the cell since it can be involved in pro-survival pathway by activation of the TRAF6 (TNF receptor-associated factor 6)-NF-κB pathway (Linares et al. 2013). On the other hand, p62 can activate caspase-8 through its polyubiquitination and association with death-inducing signaling complex (DISC), leading to cell death (Hughes et al. 2009; Singh et al. 2014). Various interactions of p62 include the stabilization of Nrf2 and activation of the transcription of Nrf2-related genes, including those which are crucial for cellular antioxidant defense (reviewed in Bryan et al. (2013)) (Johansson et al. 2015; Wang et al. 2014). Nrf2 was shown to transcriptionally regulate autophagy genes in a mouse model of Alzheimer disease (Pajares et al. 2016). As mentioned, to protect against oxidative stress, Nrf2 can cooperate with Keap1, but the activation of Nrf2 can promote autophagic degradation of Keap1 (Park et al. 2015). It seems that the role Nrf2 plays in autophagy, especially through the interaction with p62, strongly depends on the cellular context as there are many reports suggesting different actions of this protein in different cellular states (reviewed in Taniguchi et al. (2016)).
Autophagy plays a number of pro-survival functions in the cell (Fig. 2b). The production of amino acids is dependent on the availability of their components, which are normally supplied with nutrition, but in starvation, they can be delivered by increased autophagy, which results in increased amino acid production from degraded polypeptides (Vabulas and Hartl 2005). Amino acids can be included in the tricarboxylic acid (TCA) cycle to produce ATP, and autophagy was shown to play a role in this process by regulating the flow of metabolites in the TCA cycle (Guo et al. 2011). The recycling properties of autophagy—degrading of unneeded material to produce components for new blocks—make it a suitable tool for cell renovation during differentiation and development (reviewed in Mizushima and Komatsu (2011)). This is especially important just after fertilization, when the maternal material should be replaced by that encoded by the zygotic genome (Tsukamoto et al. 2008a, b). In that stage of development, autophagy plays an important role in establishing a maternal line of inheritance of mitochondrial DNA (mtDNA), since it is crucial for selective degradation of paternal mitochondria (reviewed in Sato and Sato (2013a, b)) (Zhou et al. 2011a, b). In general, degradation of mitochondria in the autophagic process is called mitophagy, and elimination of paternal mitochondria in early embryos by allogeneic organelle autophagy is called allophagy (reviewed in Sato and Sato (2013b)).
Results of several studies suggest that autophagy plays a role in the pathogenesis of various eye diseases, including those involving retinal degeneration (reviewed in Li et al. (2015)).
Autophagy—an important player in the pathogenesis of age-related macular degeneration
Not only autophagy but also proteosomal-mediated proteolysis can play a role in AMD. We showed that the expression of p62/SQSTM1 was post-transcriptionally regulated by the ELAVL1/HuR factor, when proteosomal activity was inhibited in ARPE-19 cells (Viiri et al. 2013). Therefore, p62/SQSTM1 can be considered a central protein for the involvement of disturbed autophagy and proteolysis in drusen formation and the role of this effect in AMD pathogenesis. Samples from AMD patients showed a stronger accumulation of p62/SQSTM1 rather than ELAVL1/HuR, suggesting a greater contribution of autophagy in clearing protein aggregates than proteasome-mediated proteolysis, and in this way, autophagy can be seen as the main mechanism involved in the protein aggregation in RPE cells (Fig. 3b). Since increased protein aggregation can contribute to inflammasome activation and tissue injury, it may be speculated that decreased autophagy and increased inflammation participate in drusen biogenesis (Liu et al. 2016; Wang et al. 2014) (reviewed in Wang et al. (2016a, b)).
Mitter and coworkers observed over 100% increase in the number of autophagosomes in RPE of two mouse models of AMD and human AMD donors (Mitter et al. 2014). A significant increase in the expression of the LC3, Atg7, and Atg9 proteins was observed in SOD2-knocked mice in their early stage of development. Similar increase was noted in the tissue sections from AMD donors. However, both experimental animals and AMD donors showed a decreased autophagosome fractional volume in both early and late stages of AMD as compared to controls. The results obtained in that work are important for several reasons. Firstly, increasing autophagy with age in mice RPE is somehow surprising in the context of general decrease of autophagy with age. However, at this point, two features should be distinguished: autophagy potential and autophagy activity. One can assume that overall autophagy capacity decreases with age, but this does not exclude that there is an increased autophagy flux in the initial phase of oxidative stress, when a large amount of damaged proteins and other molecules is produced. On the other hand, this can lead to overloading of the autophagosomal system, resulting in a decrease in autophagy activity. These effects can be associated with initial phase of AMD. The authors showed a dynamic character of autophagy in RPE cells in oxidative stress, which was displayed in increased and decreased autophagy evoked by acute and chronic stress, respectively. The initial increase in autophagy was attributed to increased number of autophagosomes, which can result from a reduced effectiveness of lysosomes to degrade autophagosomal content. However, the authors did not directly check this possibility, as it is technically challenging, but this feature can be associated with AMD (reviewed in Kaarniranta et al. (2013) and Mitter et al. (2012)).
Five autoreactivity targets were found in the age-related maculopathy ancillary (ARMA) study to identify and characterize human macula autoantigens in sera of AMD subjects: two belonging to heat shock protein 70 (HSP70) family HSPA8/9, another heat shock protein HSPB4, also known as alpha-crystallin A chain (CRYAA), Annexin A5, and Protein S100-A9 (Iannaccone et al. 2015). Although these proteins can be involved in various effects and mechanisms important for AMD development, including immunomodulation and antioxidative and anti-apoptotic protection, they all can participate in autophagy activation, confirming an important role of this process in AMD pathogenesis.
The critical role of autophagy in the homeostasis of aging RPE was shown in mice with knockout in the gene coding for the RB1CC1/FIP200 protein, which is essential for autophagy induction (Yao et al. 2015). These mice developed age-related degeneration of RPE, which was featured by atrophic patches, subretinal migration of microglia, deposition of oxidatively damaged proteins, and choroidal neovascularization. Degenerated RPE was linked with the loss of neighboring photoreceptors. Although it is known that A2E, a component of lipofuscin exerting a toxic effect on RPE cells, is deposited in these cells with age, its exact mechanism contributing to AMD pathogenesis is not completely known. It was shown that A2E induced autophagy and decreased the viability of RPE cells, indicating another mechanism of the involvement of autophagy to AMD pathogenesis (Guha et al. 2013; Zhang et al. 2015). In general, the interrelationships between autophagy-lysosomal and the ubiquitin-proteosome pathways can be important for the pathogenesis of AMD, in which accumulation of non-functional proteins is observed (Zhan et al. 2016). It was observed that impaired autophagy resulted in a decreased proteosomal-mediated proteolysis, suggesting that autophagy can be a principal system for clearance of misfolded and otherwise damaged proteins in human RPE cells.
Oxidative stress plays a role in many aspects of AMD pathogenesis, and cellular antioxidant response, which can be seen as an AMD protective mechanism, is linked to autophagy through the p62/Keap1/Nrf2 pathway (Johansson et al. 2015; Wang et al. 2014). However, interconnections between autophagy, oxidative stress, and AMD can be wider as autophagy can be associated with other elements of antioxidant defense, including DNA repair (reviewed in Czarny et al. (2015) and Filomeni et al. (2015)). As DNA damage response (DDR) can be important for AMD, its association with autophagy can play a role in AMD pathogenesis. As we showed, variability of DNA repair genes, including the base excision repair pathway hOGG1 and MUTYH genes, was associated with AMD (reviewed in Blasiak et al. (2012)) (Synowiec et al. 2012). However, the exact role of autophagy in DDR or rather an association between these two phenomena was not determined. Likely, the most direct link between them is expressed by mitophagy, when mitochondria with highly damaged DNA are degraded (Kurihara et al. 2012). This is a specific feature of DDR in mitochondria, as in the nucleus, seriously damaged DNA can induce apoptosis or, in certain circumstances, be tolerated resulting in mutations (reviewed in Sharma et al. (2013)). Although it is known that reactive oxygen species (ROS) can induce autophagy in starvation conditions, it is not known which exactly species are responsible for this effect (reviewed in Filomeni et al. (2010)). Both superoxide radical (O2 •–) and hydrogen peroxide (H2O2) were considered species involved in autophagy triggering in starvation conditions (Chen et al. 2009; Scherz-Shouval et al. 2007a; Scherz-Shouval et al. 2007b; Zhang et al. 2013). In general, ROS can be considered inducers of autophagy (reviewed in Azad et al. (2009; Levonen et al. 2014). Moreover, some data suggest that mitochondria are the main source of ROS needed for the induction of autophagy (Scherz-Shouval et al. 2007a; Scherz-Shouval et al. 2007b) (reviewed in Scherz-Shouval and Elazar (2007)). Therefore, it seems that there is a logical chain of events: starvation increases energetic demands, which in turn increase ATP production in mitochondria and elevate ROS level. However, the action of ROS, especially in oxidative stress, can induce also other mechanisms, which can interfere with autophagy.
A diet rich in polyunsaturated fatty acid (PUFA) is considered an AMD risk factor, but the question whether it is an independent factor is not completely answered (reviewed in Lambert et al. (2016)). However, one of the mechanisms underlying this association can result from the peroxidation of PUFA as it results in the formation of lipid peroxidation products, which can be detrimental for the cell (reviewed in Ayala et al. (2014)). This effect can be associated with wet AMD as it was observed that malondialdehyde (MDA), a major lipid peroxidation product, induced VEGF secretion, which was associated with choroidal neovascularization, typical for wet AMD (Ye et al. 2016). Moreover, MDA also induced autophagy impairment in AMD patients and ARPE-19 cells, observed as changes in the expression of Beclin 1, LC3B, and p62 proteins. Therefore, MDA accumulation can be an important step in AMD pathogenesis related to oxidative stress and autophagy.
Autophagy, apoptosis, pyroptosis, and necroptosis in AMD
Autophagy improves cell survival through the recycling of metabolic precursors and removing damaged proteins and organelles from the cell. Additionally, autophagy modulates inflammation responses and cell death pathways and thereby influences disease pathogenesis, including AMD (reviewed in Kaarniranta et al. (2013) and Nikoletopoulou et al. (2013)). Autophagy inhibition resulted in inflammasome activation and increased angiogenesis in RPE cells exposed to rotenone, an inhibitor of the electron transport chain in mitochondria (Liu et al. 2016). Inhibited autophagy induced caspase-3-mediated cell death, suggesting that apoptosis can be over positive control of autophagy in RPE cells.
Hydrogen peroxide at high concentrations induced necrotic cell death, mediated by the activation of poly(ADP-ribose) polymerase 1 (PARP1) in human RPE cells in culture, and nicotinamide adenine dinucleotide (NAD+) protected the cells against this effect (Zhu et al. 2016). Using autophagy inhibitors, it was shown that autophagy was responsible for that protective effect. Therefore, two important compounds can play a role in the regulation of cellular death in AMD: NAD+ and PARP1, and their action can be related to autophagy. Ultrastructural pathology studies suggest that the predominant mechanisms of cellular death in AMD were pyroptosis and necroptosis, while apoptosis could have a minor contribution (reviewed in Ardeljan et al. (2014)).
Mitochondria seem to be an important element of regulation of cellular death in AMD for several reasons. Firstly, they are important for the intrinsic apoptosis pathway (Criollo et al. 2007) (reviewed in Kroemer et al. (2007)). Secondly, they are linked to inflammatory response and pyroptosis (Zhou et al. 2011a, b). Thirdly, the process of cell degeneration, crucial for acquiring AMD phenotype, can be associated with dysfunctional mitochondria (reviewed in Carelli et al. (2002)). Autophagy proteins can inhibit inflammasome activation by reducing the release of mitochondrial DNA (Nakahira et al. 2011).
As AMD is associated with inflammation, pyroptosis could be initiated at its specific level. This was confirmed by the presence of caspase-1 in the NLRP3 inflammasome (Wang et al. 2016a, b). Results of some studies suggest that pyroptosis can be functionally linked with autophagy. It was shown that inhibition of autophagy stimulated pneumococcus-induced pyroptosis and protected microglia cells against pyroptosis (Kim et al. 2015). Extensive studies led by Hanus et al. (2015b) suggest that necroptosis can dominate in RPE cell death associated with dry AMD.
The involvement of DICER1 in the degeneration of RPE cells can be independent of its role in microRNA (miRNA) biogenesis. However, it was reported that DICER1 and the EIF2CA/AGO complex, which is essential for miRNA homeostasis, could be degraded by autophagy, if not loaded with miRNA (reviewed in Gibbings et al. (2013)). The degradation resulted from the recognition by the selective autophagy receptor CALCOCO2/NDP52 (calcium-binding and coiled-coil domain 2/nuclear dot protein, 52 kDa). Therefore, autophagy can be important in the regulation of the pool of miRNA-free DICER1, which could target Alu RNA, and this mechanism can contribute to AMD pathogenesis. Several interconnections between autophagy and miRNA biogenesis were reported in cancer and other diseases and aging, so it would be useful to relate these results to AMD (Bao et al. 2016; Tan et al. 2016b; Zhang et al. 2016).
Conclusions and perspectives
AMD is a major eye disease and emerging health problem in developed countries with no remedy in general. Studies on the mechanisms of AMD pathogenesis are crucial for effective treatment of this disease. As AMD is a degenerative disease, cell death underlies its phenotypic expression. However, the precise mechanism regulating the death of RPE cells and photoreceptors, crucial for AMD progression, is not known. It should be taken into account that such mechanism could not be represented by a simple chain of events, as AMD is a highly non-homogenous disease with various phenotypes and severity. The most basic question is whether AMD-affected cells undergo programmed death or necrosis? Many reports indicate necrosis as the main mode of RPE cell death in AMD, but some of them describe rather its programmed version, necroptosis, than fully uncontrolled cell death (reviewed in Galluzzi et al. (2011) and Vandenabeele et al. (2010)) (Holler et al. 2000; Murakami et al. 2014). Therefore, programmed cellular death should be rather linked with AMD pathogenesis.
Exploring the role autophagy plays in AMD pathogenesis, it would be justified to look for the association between known AMD susceptibility genes and autophagy. Genome-wide association study (GWAS) as well as earlier studies on AMD genetics revealed many genes, which variability can be associated with AMD occurrence and progression. GWAS and Exome-Chip attitudes allowed for the identification of more than 50 common and rare variants at more than 30 loci (Fritsche et al. 2016). They can be classified in several ways. One of them categorizes AMD susceptibility genes into five main categories: inflammation and immune response, cell stress response, lipid metabolism and transport, extracellular matrix and cell adhesion, and angiogenesis (reviewed in Tan et al. (2016a, b)). It should be noted that the causative role of these genes in AMD pathology is not fully known. Whether any of these genes can be closely related to autophagy is an open question. We showed that ADAMTS9 locus can be considered a candidate for such an association (Helisalmi et al. 2014). However, although there is no solid evidence for link of autophagy with AMD susceptibility, some autophagy-related genes can play a role in AMD pathogenesis as they are important for some general phenomena occurring in the AMD course, and in this review, we tried to show that autophagy can be involved in AMD-associated RPE cell death.
Therapeutic strategy targeting the mechanism regulating RPE cell death would be useful as it could be a universal strategy for AMD. At present, the only effective treatment for AMD is based on the use of anti-VEGF in the wet form of the disease. There is no remedy for GA in the dry AMD. However, as we tried to stress in our review, the mechanism of determination of RPE cell death is far from completeness. We tried to show that autophagy was involved in most cases of AMD-related death of RPE cells. This involvement is underlined by the role inflammation plays in AMD pathogenesis and regulation of the NLRP3 inflammasome, playing a central role in both dry and wet forms of the disease, by autophagy. Therefore, research on the inhibition of NLRP3 activation by autophagy proteins should search for detailed mechanisms of this interaction, projecting some chemical or/and genetic modulators of this interaction.
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