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Labile substrate availability controls temperature sensitivity of organic carbon decomposition at different soil depths

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Abstract

The decomposition of soil organic carbon (SOC) is intrinsically sensitive to temperature. However, the degree to which the temperature sensitivity of SOC decomposition (as often measured in Q10 value) varies with soil depth and labile substrate availability remain unclear. This study explores (1) how the Q10 of SOC decomposition changes with increasing soil depth, and (2) how increasing labile substrate availability affects the Q10 at different soil depths. We measured soil CO2 production at four temperatures (6, 14, 22 and 30 °C) using an infrared CO2 analyzer. Treatments included four soil depths (0–20, 20–40, 40–60 and 60–80 cm), four sites (farm, redwood forest, ungrazed and grazed grassland), and two levels of labile substrate availability (ambient and saturated by adding glucose solution). We found that Q10 values at ambient substrate availability decreased with increasing soil depth, from 2.0–2.4 in 0–20 cm to 1.5–1.8 in 60–80 cm. Moreover, saturated labile substrate availability led to higher Q10 in most soil layers, and the increase in Q10 due to labile substrate addition was larger in subsurface soils (20–80 cm) than in surface soils (0–20 cm). Further analysis showed that microbial biomass carbon (MBC) and SOC best explained the variation in Q10 at ambient substrate availability across ecosystems and depths (R2 = 0.37, P < 0.001), and MBC best explained the variation in the change of Q10 between control and glucose addition treatment (R2 = 0.14, P = 0.003). Overall, these results indicate that labile substrate limitation of the temperature sensitivity of SOC decomposition, as previously shown in surface soils, is even stronger for subsoils. Understanding processes controlling the labile substrate availability (e.g., with rising atmospheric CO2 concentration and land use change) should advance our prediction of the fate of subsoil SOC in a warmer world.

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Acknowledgments

We thank Johanna Pausch, Amy Concilio, and Lindsey Kelly for laboratory assistance, and anonymous reviewers and associate editors for their insightful comments on earlier versions of our manuscript. This study was supported by grants from the U.S. Department of Energy’s Office of Science through the Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University (DE-FC02-06ER64158), the U.S. National Science Foundation, Division of Environmental Biology’s Ecosystem Studies Program (DEB-1354098), the Overseas Foundation of the Chinese Academy of Sciences, National Science Foundation of China (31270492), and the Office of 985 Project at Peking University.

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    1. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization of Chinese Academy of Sciences, and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu, 610041, China

      Xueyong Pang

    2. Department of Ecology, College of Urban and Environmental Sciences, and Key Laboratory for Earth Surface Processes of the Ministry of Education, Peking University, Beijing, 100871, China

      Biao Zhu

    3. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China

      Xiaotao Lü & Weixin Cheng

    4. Department of Environmental Studies, University of California, Santa Cruz, CA, 95064, USA

      Weixin Cheng

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    1. Xueyong Pang
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    Correspondence to Weixin Cheng.

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    Xueyong Pang and Biao Zhu contributed equally to this work.

    Responsible Editor: Marc Kramer.

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    Pang, X., Zhu, B., Lü, X. et al. Labile substrate availability controls temperature sensitivity of organic carbon decomposition at different soil depths. Biogeochemistry 126, 85–98 (2015). https://doi.org/10.1007/s10533-015-0141-0

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