Archives of Virology

, Volume 164, Issue 1, pp 105–116 | Cite as

Isolation, identification, and classification of a novel rhabdovirus from diseased Chinese rice-field eels (Monopterus albus)

  • Wenzhi Liu
  • Yuding Fan
  • Zhong Li
  • Jianqing Zhao
  • Yong Zhou
  • Nan Jiang
  • Jia Zeng
  • Kenneth Cain
  • Lingbing ZengEmail author
Original Article


In 2017, a clinical disease outbreak resulted in substantial mortality of adults and larvae of cultured Chinese rice-field eels (Monopterus albus) on a farm in Hubei, Central China. A rhabdovirus was isolated from moribund specimens, and typical clinical symptoms associated with an outbreak included an enlarged and swollen head. This differed from previous observations. Histological changes included necrosis and cavities of various sizes within the brain and kidney. Homogenized tissues of diseased Chinese rice-field eels were screened for viral isolation using six different fish cell lines. A rhabdovirus was isolated following observation of cytopathic effect (CPE) in a gibel carp brain (GiCB) cell line and confirmed by RT-PCR. Electron microscopy showed large numbers of rhabdovirus-shaped particles in the cytoplasm of the brain cells of the diseased Chinese rice-field eels and in the infected GiCB cell line. This virus has been named “Chinese rice-field eel rhabdovirus” (CrERV), and the complete nucleotide sequence of CrERV was cloned. This rhabdovirus is composed of 11,545 nucleotides with the following genomic organization: 3′-N-P-M-G-L-5′. The genes are separated by conserved gene junctions, and phylogenetic analysis of the L sequence revealed that CrERV forms a separate branch with Siniperca chuatsi rhabdovirus (SCRV) and hybrid snakehead rhabdovirus C1207 (HSHRV-C1207). This is the first report of the complete sequence of CrERV from the Chinese rice-field eel in China.



This work was supported by the Central Public-Interest Scientific Institution Basal Research Fund, CAFS (grant numbers: No. 2018JBF01; NO. 2017HY-ZD1005) and the Science and Technology Support Program of Hubei Province (grant number: 2015BBA234).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The study was performed in strict accordance with the Guide for the Care and Use of Laboratory Animals Monitoring Committee of Hubei Province, China, and the protocol was approved by the Committee on the Ethics of Animal Experiments at the Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences. The Chinese rice-field eels were euthanized for 20–30 min in 1 mg of MS-222 (Sigma) per ml to limit their suffering before tissue collection.


  1. 1.
    Zhou R, Cheng H, Zhang Q, Guo Y, Richard RC, Terrence RT (2002) SRY-related genes in the genome of the rice field eel (Monopterus albus). Genet Sel Evo l 34:129–137CrossRefGoogle Scholar
  2. 2.
    Jang S, Zhou F, Xia L, Zhao W, Cheng H, Zhou R (2006) Construction of a BAC library and identification of Dmrt1gene of the rice field eel, Monopterus albus. Biochem Biophys Res Commun 348:775–780CrossRefPubMedGoogle Scholar
  3. 3.
    Xu QQ, Wan J, Pan TS, Zhou JW, Ai KT, Yang DQ (2015) Antiviral genes in the Asian swamp eel Monopterus albus: comparison of tissue expression and inducible expression of six interferon regulatory factors. Genes Genom 37:429–440CrossRefGoogle Scholar
  4. 4.
    Yin SW, Liu Y (2010) Compositions and functions of the hatching froth from rice field eel (Monopterus albus, Zuiew). Fish Physiol Biochem 36:195–200CrossRefPubMedGoogle Scholar
  5. 5.
    Zou JX (1996) Artificial propagation and embryonic development of Monopterus albus. Technol Inf 23:27–30 (in Chinese) Google Scholar
  6. 6.
    Zhou QB, Wu HD, Zhu CS, Yan XH (2011) Effects of dietary lipids on tissue fatty acids profile, growth and reproductive performance of female rice field eel (Monopterus albus). Fish Physiol Biochem 37:433–445CrossRefPubMedGoogle Scholar
  7. 7.
    Qu GC (2003) Reasons for the large number of deaths in the early stage of stocking of Monopterus albus and the measures to be taken. Fishery Guide to be Rich 14:48–49Google Scholar
  8. 8.
    Hu BL (2003) Techniques of artificial propagation and seedling cultivation in Asian swamp eel. Fishery Guide Rich 17:31–32Google Scholar
  9. 9.
    Chai JY, Sohn WM, Na BK, Park JB, Jeoung HG, Hoang EH, Htoon TT, Tin HH (2015) Larval Gnathostoma spinigerum detected in Asian Swamp eels, Monopterus albus, purchased from a local market in Yangon, Myanmar. Korean J Parasitol 5:619–625CrossRefGoogle Scholar
  10. 10.
    Shao JC, Yuan JF, Shen YL, Hu RX, Gu ZM (2016) First isolation and characterization of Edwardsiella tarda from diseased Asian swamp eel, Monopterus albus (Zuiew). Aquac Res 47:3684–3688CrossRefGoogle Scholar
  11. 11.
    Ou T, Zhu RL, Chen ZY, Zhang QY (2013) Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus. Dis Aquat Organ 106:197–206CrossRefPubMedGoogle Scholar
  12. 12.
    Xu J, Zeng LB, Zhang H, Zhou Y, Ma J, Fan YD (2013) Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China. Vet Microbiol 166:138–144CrossRefPubMedGoogle Scholar
  13. 13.
    Tao JJ, Zhou GZ, Gui JF (2008) Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF. Virus Res 132:86–96CrossRefPubMedGoogle Scholar
  14. 14.
    Bjorklund HV, Higman KH, Kurath G (1996) The glycoprotein genes and gene junctions of the fish rhabdoviruses spring viremia of carp virus and hirame rhabdovirus: analysis of relationships with other rhabdoviruses. Virus Res 42:65–80CrossRefPubMedGoogle Scholar
  15. 15.
    Gadd T, Viljamaa DS, Holopainen R, Koski P, Jakava VM (2013) Characterization of perch rhabdovirus (PRV) in farmed grayling Thymallus thymallus. Dis Aquat Org 106:117–127CrossRefPubMedGoogle Scholar
  16. 16.
    Poch O, Blumberg BM, Bougueleret L, Tordo N (1990) Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol 71:1153–1162CrossRefPubMedGoogle Scholar
  17. 17.
    Wagner RR, Rose JK (1996) Rhabdoviridae: the viruses and their replication. Fields Virol 3:1221–1244Google Scholar
  18. 18.
    Walker PJ, Blasdell K, Calisher CH, Dietzgen RG, Kondo H, Kurath G, Longdon B, Stone DM, Tesh RB (2018) ICTV Virus Taxonomy Profile: Rhabdoviridae. J Gen Virol. PubMedGoogle Scholar
  19. 19.
    Assenberg R, Delmas O, Morin B, Graham SC, De Lamballerie X, Laubert C, Coutard B, Grimes JM, Neyts J (2010) Genomics and structure /function studies of Rhabdoviridae proteins involved in replication and transcription. Antiviral Res 87:149–161CrossRefPubMedGoogle Scholar
  20. 20.
    Schütze H, Mundt E, Mettenleiter TC (1999) Complete genomic sequence of viral haemorrhagic septicemia virus, a fish rhabdovirus. Virus Gen 19:59–65CrossRefGoogle Scholar
  21. 21.
    Ivanov I, Yabukarski F, Ruigrok RW, Jamin M (2011) Structural insights into the rhabdovirus transcription/replication complex. Virus Res 162:126–137CrossRefPubMedGoogle Scholar
  22. 22.
    King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus Taxonomy: Classification and Nomenclature of Viruses. Refer. Res. Book News 4:1730–1756Google Scholar
  23. 23.
    Morzunov SP, Winton JR, Nichol ST (1995) The complete genome structure and phylogenetic relationship of infectious hematopoietic necrosis virus. Virus Res 38:175–192CrossRefPubMedGoogle Scholar
  24. 24.
    Richard G, Steven B, Elsa A (2012) Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel. Virus Res 166:1–12CrossRefGoogle Scholar
  25. 25.
    Padhi A, Verghese B (2012) Molecular evolutionary and epidemiological dynamics of a highly pathogenic fish rhabdovirus, the spring viremia of carp virus (SVCV). Vet Microbiol 156:54–63CrossRefPubMedGoogle Scholar
  26. 26.
    Liu XD, Wen Y, Hu XQ, Wang WW, Liang XF, Li J, Vikram V, Lin L (2015) Breaking the host range: mandarin fish is susceptible to a vesiculovirus derived from snakehead fish. J Gen Virol 96:775–781CrossRefPubMedGoogle Scholar
  27. 27.
    Zeng WW, Wang Q, Wang YY, Liu C, Liang HR, Fang X, Wu SQ (2013) Genomic characterization and taxonomic position of a rhabdovirus from a hybrid snakehead. Arch Virol 159:2469–2473CrossRefGoogle Scholar
  28. 28.
    Sohrab A, Mehdi S, Karim M, Sara S, Hooman RH (2016) Isolation and identification of viral hemorrhagic septicemia virus (VHSV) from farmed rainbow trout (Oncorhynchus mykiss) in Iran. Acta Tropica 156:30–36CrossRefGoogle Scholar
  29. 29.
    Fu XZ, Lin Q, Liang H, Liu L, Huang Z, Li N, Su J (2017) The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Arch Virol 162:2829–2834CrossRefPubMedGoogle Scholar
  30. 30.
    Zhang Q, Li Z (1999) Three viruses was observed in diseased Chinese perch Siniperca chuatsi. Chin Sci Bull 44:192–195Google Scholar
  31. 31.
    Ammayappan A, Vakharia VN (2009) Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA. Virol J 6:171–187CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Biacchesi S (2010) The reverse genetics applied to fish RNA viruses. Vet Res 42:12CrossRefGoogle Scholar
  33. 33.
    Spiropoulou CF, Nichol ST (1993) A small highly basic protein is encoded in overlapping frame within the P gene of vesicular stomatitis virus. J Virol 67:3103–3110PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Yangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhanChina
  2. 2.College of FisheriesHuazhong Agricultural UniversityWuhanChina
  3. 3.Department of Fish and Wildlife Sciences and the Aquaculture Research InstituteUniversity of IdahoMoscowUSA

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