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Production of individual ganoderic acids and expression of biosynthetic genes in liquid static and shaking cultures of Ganoderma lucidum

  • Biotechnological Products and Process Engineering
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Abstract

Two-stage culture was efficient in enhancing total ganoderic acid (GA) production by Ganoderma lucidum (Fang and Zhong, Biotechnol Prog 18:51–54, 2002). As different GAs have different bioactivities, it is critical to understand the kinetics of individual GA production during fermentation, but no related information is yet available. To understand the regulation of GA biosynthesis, investigation of the accumulation of intermediate (lanosterol) and by-product (ergosterol) and of the expression of three important biosynthetic genes was also conducted in liquid shaking and static cultures of G. lucidum. The results showed that the content of individual GAs increased rapidly in the liquid static culture, and their maximum value was 6- to 25-fold that of shaking culture while lanosterol content in the former was lower than the latter. The transcript of squalene synthase (SQS), lanosterol synthase and 3-hydroxy-3-methylglutaryl coenzyme A reductase in liquid static culture was 4.3-, 2.1-, and 1.9-fold that of the shaking culture, respectively. Higher GA content in liquid static culture was related to increased transcription of those genes especially SQS. The work is helpful to the production of individual GAs and provided an insight into why the liquid static culture was superior to the shaking culture in view of biosynthetic gene expression.

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Acknowledgment

Financial support from the National Natural Science Foundation of China (NSFC project no. 20776084, no. 30821005), the National High Technology R&D Program (863 project no. 2007AA021506), and the Shanghai Leading Academic Discipline Project (project nos. B203 and B505) is gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Jun-Wei Xu & Jian-Jiang Zhong

  2. Key Laboratory of Microbial Metabolism (Ministry of Education), School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China

    Yi-Ning Xu & Jian-Jiang Zhong

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  1. Jun-Wei Xu
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  2. Yi-Ning Xu
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Correspondence to Jian-Jiang Zhong.

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Xu, JW., Xu, YN. & Zhong, JJ. Production of individual ganoderic acids and expression of biosynthetic genes in liquid static and shaking cultures of Ganoderma lucidum . Appl Microbiol Biotechnol 85, 941–948 (2010). https://doi.org/10.1007/s00253-009-2106-5

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  • DOI: https://doi.org/10.1007/s00253-009-2106-5

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