Abstract
The Kumamoto oyster (Crassostrea sikamea) shows a spatially restricted distribution, favoring estuarine tideland environment. On the other hand, the Pacific oyster (C. gigas) has a broader range of habitat. The present study compared the mitochondrial population structure between the two closely related species. For accurate species identification of oysters sampled from Japanese and East Asian continental coasts, we performed sequencing analysis of the mitochondrial DNA (mtDNA) and PCR-RFLP assay of the first internal transcribed spacer of nuclear rRNA genes. Then, we estimated the extent of population differentiation within each of C. sikamea and C. gigas based on the mtDNA data. Few haplotypes were shared among the sites of sampling in C. sikamea, which contrasted with an extensive haplotype sharing among C. gigas samples. We discuss the mechanisms of elevated population differentiation observed in C. sikamea in light of the ecology and the ancient ocean geography around the present-day habitats.
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Acknowledgments
We are grateful to professor D. Hedgecock, University of Southern California, for kindly reading the earlier draft of the manuscript and providing many insightful comments. Many constructive suggestions from two anonymous reviewers and handling editor helped improve the manuscript. We also thank Mr. K. Kitahara of Seikai Yoshoku Giken and Dr. R. Fuseya of National Research Institute of Fisheries Engineering, Fisheries Research Agency of Japan, for their assistance to MS’s expedition to Ariake Sea and Omura Bay for sample collection. QL’s graduate students helped MS to collect oysters in Qingdao.
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Sekino, M., Sato, S., Hong, JS. et al. Contrasting pattern of mitochondrial population diversity between an estuarine bivalve, the Kumamoto oyster Crassostrea sikamea, and the closely related Pacific oyster C. gigas . Mar Biol 159, 2757–2776 (2012). https://doi.org/10.1007/s00227-012-2037-z
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DOI: https://doi.org/10.1007/s00227-012-2037-z