Positive influence of Methotrexate-Hydroxychloroquine combination on the expression of GM-CSF receptor on neutrophils of synovial fluid in rheumatoid arthritis
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Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) has been inducted as a mediator of inflammation in rheumatoid arthritis. Methotrexate combination therapy forms an important component of the treatment regimen in rheumatoid arthritis. The present study was undertaken to evaluate the influence of Methotrexate-Hydroxychloroquine (MTX-HCQ) combination and Sulfsalazine- Hydroxychloroquine (SSZ-HCQ) combination on the expression GM-CSFR in neutrophils isolated from synovial fluids. 15 cases of confirmed rheumatoid arthritis patients who presented at the hospital for surgical correction of joint deformities were selected for the study. Neutrophils isolated from the synovial fluids were used as the source of the receptor for quantitation on an enzyme immunoassay (EIA). The EIA was developed and standardized in our laboratory for quantification of the GM-CSF R. The findings are suggestive of the fact that the administration of MTX-HCQ combination has positive influence on the expression of the GM-CSF R on neutrophils as against SSZ-HCQ combination. The physiological basis of this increase needs further investigation.
Key wordsMethotrexate Rheumatoid arthritis GM-CSF receptor Synovial fluid
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- 5.Janeway, C.A., Travers, P., Walport, M., Schlomchik, M. (2001) Immunobiology; 5th edn. Garland Publishing.Google Scholar
- 6.Di, Persio, J., Billing, P., Kaufman, S., Eghtesady, P., Williams, R.C. and Casson, J.C. (1988) Characterisation of the human granulocyte macrophage colony stimulating receptor. J. Biol. Chem. 263(4), 1834–41.Google Scholar
- 8.Di Persio, J.F., Golde, D.W. and Gasson, J.D. (1990) GM-CSF: receptor structure and transmembrane signaling. Int. J. Cell-Cloning 74(5), 63–74.Google Scholar
- 9.Sidney, P., Colowick and Nathan, O., Kaplan (1984) Methods in Enzymology 105, 359–362.Google Scholar