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Recovery of natural insecticides fromTephrosia vogelii.

II. Toxicological properties of rotenoids extracted from fresh and oven-dried leaves

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Summary

Bioassays, using guppies as a test animal, were conducted onTephrosia leaf extracts of seven plants obtained by four different extraction methods. The total rotenoids recovered by acetone extraction of fresh leaves appeared to have slightly more effective piscicidal properties than rotenoids recovered by acetone extraction of oven-dried leaves. These differences, although not statistically significant, proved the validity of using fresh leaf extraction for the evaluation of rotenoid content inTephrosia vogelii leaves.

Individual plants varied significantly with regard to their toxicological effectiveness, thereby demonstrating that the proportion of rotenone vs. other rotenoids varies amongTephrosia plants. ATephrosia breeding program cannot rely solely on the red-color test as a measure of rotenone equivalents but must be supplemented with periodic bioassays to assess the pesticidal effectiveness of the breeding material.

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Literature Cited

  1. Barnes, D. K., and R. H. Freyre. 1966. Recovery of natural insecticide fromTephrosia vogelii. I. Efficiency of rotenoid extraction from fresh and ovendried leaves. Econ. Bot.20(3).

  2. Irvine, J. E., and R. H. Freyre. 1959. The occurrence of rotenoids in some species of the genusTephrosia. Jour. Agr. Food Chem.7: 106–107.

    Article  CAS  Google Scholar 

  3. —, and —. 1959. Varietal differences in the rotenoid content ofTephrosia vogelii. Agron. Jour51: 664–665.

    Google Scholar 

  4. Jones, M. A. 1945. Application of a modified red-color test for rotenone and related compounds ofDerris andLonchocarpus. Assoc. Off. Agr. Chem. Jour.28: 352–359.

    CAS  Google Scholar 

  5. —, W. A. Gersdorff, and E. R. McGovran. 1946. A toxicological comparison ofDerris andLonchocarpus. Jour. Econ. Entomol.39: 281–283.

    CAS  Google Scholar 

  6. Pagán, C. 1948. The use of guppies in the toxicological assay ofDerris andLonchocarpus roots. Jour. Econ. Entomol.41: 942–945.

    Google Scholar 

  7. —, and A. J. Loustalot. 1948. A simple rapid method for estimating toxicity (rotenone equivalent) ofDerris root. Jour. Agr. Res.77: 271–277.

    Google Scholar 

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Authors and Affiliations

  1. Plant Industry Station, Beltsville, Maryland

    Donald K. Barnes (Research Geneticist)

  2. Federal Experiment Station, Crops Research Division, Agricultural Research Service, United States Department of Agriculture, Mayaguez, Puerto Rico

    Ruben H. Freyre (Research Agronomist)

Authors
  1. Donald K. Barnes
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  2. Ruben H. Freyre
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Barnes, D.K., Freyre, R.H. Recovery of natural insecticides fromTephrosia vogelii. . Econ Bot 20, 368–371 (1966). https://doi.org/10.1007/BF02904057

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  • DOI: https://doi.org/10.1007/BF02904057

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