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Diagnostic role of the antibody response to the 38kDa, 16kDa proteins and lipoarabinomannan of mycobacterium tuberculosis

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Abstract

The antibody response to the 38kDa, 16kDa and Lipoarabinomannan (LAM) antigens ofMycobacterium tuberculosis was evaluated using three different ELISAs based on these antigens. The study group included tuberculosis patients (n=52), patients with HIV and TB co-infection (n=10), other chest symptomatics (n=5), HIV infected individuals (n=10), leprosy cases (n=7) and healthy controls (n=75). The results indicate that the 38kDa and LAM based ELISA for IgM/IgG has a low specificity (ranging from 69–85%) and sensitivity (ranging from 55–78%). When three ELISAs are carried out on a single patient the probability of detection of tuberculosis was significantly increased to 95.2% indicating that a single ELISA test is of low sensitivity and that a combination of ELISA’s may be needed to be of any value as a diagnostic test for tuberculosis. Additionally, a western blot assay of the serum antibody response to protein fraction ofM. tuberculosis was analysed in 15 tuberculosis patients and five healthy controls. A multiple antibody response to various M.tuberculosis proteins was observed which varied from patient to patient as compared to controls who showed a single 38–39 kDa protein band positivity. These finding suggest that a western blot assay which determines the antibody response to a set of antigenic components ofM. tuberculosis could be a better serological test for the diagnosis of tuberculosis in our population.

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References

  1. World Health Organization. Tuberculosis fact sheet. WHO Tuberculosis site (cited 2000 Sept 15); 1(3screens), Available from; URL;http:// www.who.int/gtb/ publications/factsheet/ index.htm.

  2. Davies, P.D.O. (1997) Clinical tuberculosis. (Ed 1st). Chapman & Hall Medical Publishers.

  3. Bhatia, A.S., Kumar, S. and Harinath, B.C. (2003) Immunodiagnosis of tuberculosis: An update. Indian. J. Clin. Biochem. 18(2), 1–5.

    Article  Google Scholar 

  4. Banerjee, S., Gupta, S., Shende, N., Kumar, S. and Harinath, B.C. (2003) Serodiagnosis of tuberculosis using two ELISA systems. Indian. J. Clin. Biochem. 18(2), 48–53.

    Article  Google Scholar 

  5. Kaur, J., Arora D.R., Sikka, R. and Gupta, K.B. (1999) Evaluation of ELISA test using antigen 60 in the serodiagnosis of TB. Indian J. Med. Microbiol. 17(2), 72–73.

    Google Scholar 

  6. Chander, I., Subrahmanayam, S. and Gupta, R. (1960) Sensitivity of EIA in the diagnosis of tuberculosis using 38kDa antigen. J. Ind. Med. Assoc. 94, 376–378.

    Google Scholar 

  7. Mathai, E., Rajkumari, R., Kuruvilla, P.J., Kirubakaran, H., Brahmadathan, K.N., Inbamalar, & Abraham, O.C. (2002) Evaluation of serological tests for the diagnosis of tuberculosis. Indian J. Pathol. Microbiol. 45(4), 439–441.

    Google Scholar 

  8. Papiha, S.S., Wentzel, J., Behjati, F. and Agarwal, S.S. (1985) HLA and circulating immunoglobulin levels in Indian patients with pulmonary tuberculosis. Tubercle 66, 25–33.

    Article  PubMed  CAS  Google Scholar 

  9. Sada, E., Brennan, P.J., Herrera, T. & Torres, M. (1990) Evaluation of lipoarabinomannan for the serological diagnosis of tuberculosis. J. Clin. Microbiol. 28(12), 2587–2590.

    PubMed  CAS  Google Scholar 

  10. Wilkinson, R.J., Haslov, K., Rappuoli, R., Giovannoni, F., Narayanan, P.R., Desai, C.R., Vordermeier, H.M., Paulsen, J., Paslov, G., Ivanyi, J. & Singh, M. (1997) Evaluation of the Recombinant 38kDa antigen ofMycobacterium tuberculosis as a Potential Immunodiagnostic Reagent. J. Clin. Micro. 35(3), 553–557.

    CAS  Google Scholar 

  11. Zaher, F. & Marks, J. (1977) Methods and medium for the culture of tubercle bacilli. Tubercle. 58, 143–5.

    Article  PubMed  CAS  Google Scholar 

  12. Lodam, A.N., Reddy, M.V., Narang, P., Gupta, O.P., & Harinath, B.C. (1996) Fractionation, analysis and diagnostic utility ofMycobacterium tuberculosis H37Ra excretory-secretory antigen in pulmonary tuberculosis. Indian. J. Biochem. Biophys. 33(1), 66–71.

    PubMed  CAS  Google Scholar 

  13. Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  14. Laemmli, U.K. (1970) Cleavage of proteins during the assembly of head of bacteriophage T4. Nature 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  15. Towbin, H., Staehelin, T., & Gordon, J. (1979) Electrophorectic transfer of protein from polyacrylamide gels to NCM sheets: Procedures and some applications. Proc Natl. Acad. Sci. USA. 76, 4350.

    Article  PubMed  CAS  Google Scholar 

  16. World Health Organization. (1994) Operational characteristics of commercially available assays to detect antibodies to HIV-1 and or HIV-2 in human sera. Geneva: WHO. GPA/RID/CRD/94.4.

    Google Scholar 

  17. Cole, S.T., Eigilmeier, K., Parkhill, J., James, K.D. et al. (2001) Massive gene decay in the leprosy bacillus. Nature. 409, 1007–1011.

    Article  PubMed  CAS  Google Scholar 

  18. Van den Akker, T.W., Naafs, B., Kolk, A.H., De Glopper-van der Veer, E., Chin, R.A., Lien, A., & Van Joost, T. (1992) Similarity between mycobacterial and human epidermal antigens. Br. J. Dermatol; 127(4), 352–358.

    Article  PubMed  Google Scholar 

  19. Harboe, M. (1994) Overview of host-parasite relations. In: Robert C. Hasting, editor. Leprosy. (Ed 2nd) London: Churchill Livingstone. pp 87–111.

    Google Scholar 

  20. Rovatti, E., Corradi, M.P., Amicosante, M., Tartoni, P.L., Panini, W., Ancora, A., Cenci, A.M., Zucchi, L., Monno, L., Angarano, G., & Saltini, C. (1996) Evaluation of a western blot serum test for the diagnosis of Mycobacterium tuberculosis infection. Eur. Respir. J. 9(2), 288–92.

    Article  PubMed  CAS  Google Scholar 

  21. Franco, J., Camarena, J.J., Nogueira, J.M., Blanquer, R., Ruiz, M.J. & Marin, J. (2001) Serological response (Western blot) to fractions of Mycobacterium tuberculosis sonicate antigen in tuberculosis patients and contacts. Int. J. Tuberc. Lung. Dis. 5(10), 958–62.

    PubMed  CAS  Google Scholar 

  22. Lu, X.D., Sun, H.P., & Zhang, Y.H. (1993) Application of western blotting technique in the serodiagnosis of Mycobacterium tuberculosis infection. Zhonghua Jie He He Hu Xi Za Zhi; 16(6), 357–9, 375–6.

    PubMed  CAS  Google Scholar 

  23. Stead, W.W., Sender, J.W., Reddick, W.T. & Lofgren, J.P. (1990) Racial differences in susceptibility to infection byMycobacterium tuberculosis. New Engl J. Med. 322, 422–427.

    PubMed  CAS  Google Scholar 

  24. Smith, P.G. (1994) Clinical tuberculosis. In: Davies PDO, editor. Prevention: BCG vaccination. (Ed 1st). London: Chapman Hall; pp: 297–320.

    Google Scholar 

  25. Hoal-van Helden, E.G., Hon, D., Lewis, L.A., Beyers, N., & van Helden, P.D. (2001) Mycobacterial growth in human macrophages: variation according to donor, inoculum and bacterial strain. Cell. Biol. Int. 25(1), 71–81.

    Article  PubMed  CAS  Google Scholar 

  26. Fine, P.E.M., Floyd, S., Stanford, J.L., Nkhosa, P., Kasunga, A., Chaguluka, S., Warndorff, D.K., Jenkins, P.A., Yates, M., & Ponnighaus, J.M. (2001) Environmental mycobacteria in northern Malawi: implications for the epidemiology of tuberculosis and leprosy. Epidemiol. Infect. 126, 379–387.

    Article  PubMed  CAS  Google Scholar 

  27. UNAIDS/AIDS.AIDS epidemic update. UNAIDS, Geneva, December 2000.

  28. www.naco.nic, www.unaids.com.

  29. Walia, K. (2002) Current issues in HIV/TB co-infection. Ind. J. Tub. 49, 21–26.

    Google Scholar 

  30. Farajollahi, M.M. & Moghaddamnia, H. (2002) Development of a new capture ELISA assay of whole pathogen for diagnosis of tuberculosis in body fluids. Int. J. Tuberc. Lung Dis. 6(10), 165.

    Google Scholar 

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Authors and Affiliations

  1. Blue Peter Research Centre, LEPRA India, Hyderabad, India

    Renuka Raju, Karuna Sagili & Meher Vani C

  2. Blood Bank, Nizam Institute of Medical Sciences, (NIMS), Hyderabad

    Saraswathi V

  3. State Tuberculosis Training & Demonstration Centre, Hyderabad

    Satyanarayana A V V

  4. Nireesh Kana, HIV/AIDS Centre, 29, Hyderabad

    Sujai Suneetha

  5. Molecular Biology Laboratory Institute of Genetics, Osmania University, Begumpet, 16, Hyderabad

    Lavanya M Suneetha

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  1. Renuka Raju
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  2. Sujai Suneetha
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  3. Karuna Sagili
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  4. Meher Vani C
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  5. Saraswathi V
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  6. Satyanarayana A V V
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  7. Lavanya M Suneetha
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Correspondence to Lavanya M Suneetha.

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Raju, R., Suneetha, S., Sagili, K. et al. Diagnostic role of the antibody response to the 38kDa, 16kDa proteins and lipoarabinomannan of mycobacterium tuberculosis. Indian J Clin Biochem 20, 123–128 (2005). https://doi.org/10.1007/BF02893056

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