Skip to main content
SpringerLink
Log in

Changes in erythrocyte membrane glycoproteins during leukaemia

  • Published:
Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Changes in erythrocyte membrane glycoproteins during, leukaemia were investigated in one hundred patients. Control group contained normal ones. The total protein and protein bound total carbohydrates (neutral sugars) in the glycoproteins extracted from the erythrocyte membrane showed significant reduction in total protein and protein bound total carbohydrates before radiotherapy. However, after the radiotherapy there was further reduction in total protein by 10.41% whereas there was increase in total carbohydrates by 9.98%. Qualitative analysis reveals that with the help of lectins one can pin point the sugars which appear or disappear due to leukaemia. The test could be of a diagnostic value.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lipton, A., Harvey, H. A. Delong, S., Allegra, J., White, D., Allegoa, M. and Dandson, E. A., (1979) Glycoproteins and human cancer. I. Cancer. 47, 1966.

    Google Scholar 

  2. Harvey, H. A. Lipton, A., White, D. and Davidson, E. (1981) Glycoprotein and human cancer II. Cancer 48, 328.

    Google Scholar 

  3. Patel, P. S., Ravat, G. N., Rawal, R. M., Patel, M. M., Balar, D. B., and Patel, D. D. (1997) Importance of glycoprotein in human cancer. Biochem. Biophys, 34 (1–2) 226–233.

    CAS  Google Scholar 

  4. Elyar, H., Edvin, R., Jean, Liz. W. (1962) Oligosaccharide from the α acid glycoprotein of human plasma. J.Bio. Chem., 237, 622–628.

    Google Scholar 

  5. Fukudo, M. and Osawa, T. (1973) Isolation and characterization of a glycoprotein from human group O erythrocyte membrane. J. Biol. Chem. 248, 5100–5105.

    Google Scholar 

  6. Hasija, K. (1991) Lectin in agglutination and its role in detection of sugar on the surface of RBC of normal and cancer patients. Indian J. Clinical Biochem., 6, 89–96.

    Article  Google Scholar 

  7. Lis, H. and Sharon, N. (1973) Biochemistry of plant Lectin, Ann. Rev. Biochem., 42, 541–575.

    Article  PubMed  CAS  Google Scholar 

  8. Lis, H. and Sharon, N. (1981) Lectins in higher plants, Biochem. Plants Acad. Press.

  9. Hasija, K. and Saxena, J. S. (1986) Biochemical tests in diagnosis of diseases-1. Crystallization test in malignancy. Bio. Sc. Res. Bull. 2: 65–73.

    Google Scholar 

  10. Baxi, B. R., Patel, P. S., Adhvarya, S. G. and Day, P. K. (1991) Usefulness of serum glycoconjugates in precancerous and cancerous diseases of the oral cavity. Cancer, 67, 135–140.

    Article  PubMed  CAS  Google Scholar 

  11. Ira, S., Goldenbarg, M. D., Mark, A. and Hayes, M. D. (1958) Breast carcinoma and ABO blood group. Cancer, 2, 973–974.

    Google Scholar 

  12. Bolmer, S. D. and Davidson, E. A. (1981) Preparation and properties of glycoprotein associated with malignancy. Biochemistry, 20, 1047–1054.

    Article  PubMed  CAS  Google Scholar 

  13. Lowry, O. H., Rose Brough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with Folinphenol reagent. J. Biol. Chem., 193 265–275.

    PubMed  CAS  Google Scholar 

  14. Dubois, M., Gilies, K., Hammilton, J. K., Robers, P. A. and Smith, F. A. (1951) A Colorimetric method for the determination of sugars and related substances. Anal. Chem., 28, 350–356.

    Article  Google Scholar 

  15. Dunsford, I. and Bowley, C.C. (1967) A technique in blood grouping—Vol. 2. Olive & Boyd. Publ.

  16. Bhatia, H. M. and Boyd, W. C. (1962) Inhibitory reaction of fourteen non specific seed extracts. Transfusion, 2: 106.

    Article  PubMed  CAS  Google Scholar 

  17. Aird, I. (1955) Discussion in the ABO blood group and diseases. Proc. Roy. Soc. Med., 48, 139–140.

    PubMed  CAS  Google Scholar 

  18. Tewarson, S. L., Mittal, V. P., Singh, M. and Gupta G. P. (1993) Serum sialic acid an important cancer marker. Indian J. Cancer, 30 125–131.

    PubMed  CAS  Google Scholar 

  19. Siddiqui, F. A., Amirkhosravi, A., Amaya, M., Meyer, T., Biggerstaff, J., Desai, H. and Francis, IL. (2001) Hemoglobin enhances tissue factor expression on human malignant cell. Blood Coagul. Fibrinolysis, 12 (3), 171–177.

    Article  PubMed  CAS  Google Scholar 

  20. Tilbrook, P. A., Palmer, G. A., Bittorf, T., McCarthy, D. J., Wright, M. J., Saina, M. C., Linnekin, D., Cull, V. S., Williams, J. H., Ingley, E., Schneide-Mergener, J., Krystall, G. and Klinken, S. P. (2001) Maturation of erythroid cell and erythroleukemia development are affected by the kinase activity of lyn. Cancer Res. 15, 61 (6), 2453–2458.

    Google Scholar 

  21. Dorr, R., Karanes, C., Spier, C., Grogan, T., Greer, J., Moore, J., Weinberger, B., Schiller, G., Pearee, T., Litchman, M., Dalton, W., Roc, D. and List, A. F. (2001) Phase I/II study of the P-glycoprotein modulator PSC 833 in patients with acute myeloid leukemia. J. Clin. Oncol. 19 (6), 1589–1599.

    PubMed  CAS  Google Scholar 

  22. Magyarosy, E., Sebestypen, A. and Timar, J. (2001) Expression of metastatis associated protein, CD44v6 and NH23-HI in pediatric acute lymphoblastic leukemia. Anticancer Res., 21 (IB), 819–823.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, N.S.C.B. Govt. Medical College, 482 003, Jabalpur

    Kiran Hasija

Authors
  1. Kiran Hasija
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hasija, K. Changes in erythrocyte membrane glycoproteins during leukaemia. Indian J Clin Biochem 17, 12–19 (2002). https://doi.org/10.1007/BF02867935

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02867935

Key Words

Search

Navigation