Abstract
Theophylline in serum was estimated by three methods. Two of them are based on high pressure liquid chromatography. A reversed phase method was chosen as reference method. Liquid chromatography on a straight phase was compared with this method. A third method was based on inhibiting alkaline phosphatase enzyme activity. Samples of test serum and patients' serum were analysed. The precision and accuracy of the methods were estimated by linear regression analysis. Some other aspects like duration of analysis and cost were also investigated. Generally, straight phase liquid chromatography was the method of choice because of its simplicity, high selectivity and relatively low cost.
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References
Soeterboek AM, Jonkman JHG. Clinical pharmacokinetic aspects of theophylline. In: Merkus FWHM, ed. The serum concentration of drugs. Amsterdam: Excerpta Medica, 1980:250–9.
Danhof M, Loomans BMJ, Breimer DD. Bepaling van theofylline in plasma en speeksel, in aanwezigheid van coffeïnemetabolieten, met behulp van HPLC. Pharm Weekbl 1978:113:672–5.
Jonkman JHG, De Zeeuw RA, Schoenmaker RA. Interference in the proposed selected method for determination of theophylline by liquid chromatography. Clin Chem 1982;28:1987–8.
Muir KT, Kunitani M, Riegelman S. Improved high performance liquid Chromatographic assay for theophylline in plasma and saliva in the presence of caffeine and its metabolites and comparisons with three other assays. J Chromatogr 1982, 231:73–82.
Van Aerde Ph, Moerman E, Van Severen R, Braeckman P. Determination of plasma theophylline by straight-phase high-performance liquid chromatography: elimination of interfering caffeine metabolites. J Chromatogr 1981;222:467–74.
Vinet B, Loraine Z. Enzymic assay for serum theophylline. Clin Chem 1979:25:1370–2.
Westgard JO, Hunt MR. Use of interpretation of common statistical tests in method-comparison studies. Clin Chem 1973;19:49–57.
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Van Oostveen, J.J.J., Kerkhof, B., Scholtens, J.R. et al. Determination of theophylline in serum by a simple enzyme method and by liquid chromatography. Pharmaceutisch Weekblad Scientific Edition 7, 181–185 (1985). https://doi.org/10.1007/BF02307574
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DOI: https://doi.org/10.1007/BF02307574