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Dehydration affects cardiovascular nitric oxide synthases and caveolins in growing rats

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Abstract

Purpose

During the postnatal stage, cardiovascular nitric oxide (NO) system and caveolins (cav) may be regulated differentially in response to hypovolemic state induced by water restriction. Our aim was to examine the effects of water restriction on NO synthases (NOS) and cav in the atria, ventricle and aorta of growing rats.

Methods

Male Sprague–Dawley rats aged 25 and 50 days were divided into (n = 15): WR: water restriction 3 days; WAL: water ad libitum 3 days. Systolic blood pressure, NOS activity and NOS/cav protein levels were measured.

Results

Dehydration induced a larger increase in SBP in WR25 group. Ventricular NOS activity, endothelial NOS (eNOS) and neuronal isoform (nNOS) of WR25 pups were increased, and both cav were decreased. In the WR50 group, NOS activity remained unchanged. In the atria, NOS activity, eNOS and nNOS decreased in WR25 associated with increased cav-1; in the WR50 group, NOS activity was increased without changes in NOS isoforms. In the aorta of WR25, NOS activity and inducible NOS (iNOS) were decreased; NOS activity was unchanged in WR50, despite the decreased levels of eNOS and increased iNOS, cav-1 and cav-3.

Conclusions

NO system adjustments in cardiovascular system under osmotic stress in vivo depend on postnatal age, being eNOS and nNOS, the isoforms that determine NOS activity in cardiac tissue in 25-day-old pups. Changes in cav abundance during hypovolemic state may contribute to age-related NO production.

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Acknowledgments

This study was supported by the University of Buenos Aires, Argentina, Grant 20020100100068. The authors wish to thank Mara Marchione and Laura Alencastro for technical assistance. The authors declare no conflict of interest.

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Authors and Affiliations

  1. Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, IQUIMEFA-CONICET, Junín 956, C1113AAD, Ciudad Autónoma De Buenos Aires, Argentina

    Vanina A. Netti, Agustina N. Iovane, Mariana C. Vatrella, Andrea L. Fellet & Ana María Balaszczuk

  2. Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Ciudad Autónoma De Buenos Aires, Argentina

    Natalia D. Magnani & Pablo A. Evelson

  3. Laboratorio de Fisiopatogenia, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1113AAD, Ciudad Autónoma De Buenos Aires, Argentina

    Elsa Zotta

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  1. Vanina A. Netti
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  2. Agustina N. Iovane
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  3. Mariana C. Vatrella
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  6. Elsa Zotta
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  7. Andrea L. Fellet
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  8. Ana María Balaszczuk
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Correspondence to Vanina A. Netti.

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Netti, V.A., Iovane, A.N., Vatrella, M.C. et al. Dehydration affects cardiovascular nitric oxide synthases and caveolins in growing rats. Eur J Nutr 55, 33–43 (2016). https://doi.org/10.1007/s00394-014-0820-y

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  • DOI: https://doi.org/10.1007/s00394-014-0820-y

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