Pharmaceutisch Weekblad

, Volume 8, Issue 4, pp 234–238 | Cite as

Colorimetric determination of thioxanthenes with tetracyanoethylene

  • M. I. Walash
  • M. Rizk
  • A. El-Brashy
Original Articles


A simple and rapid colorimetric method for the quantitative determination of the thioxanthene derivatives, chlorprothixene, tiotixene and metixene has been performed either in their pure form or in tablets. The procedure is based upon the formation of charge transfer complexes with the π-acceptor tetracyanoethylene. The spectra of the complex show maxima at 415, 410 and 390 nm with a high apparent molar absorptivity which facilitates the determination of 5–100 μg·ml−1 of the studied compounds. The mean recoveries are 100.1± 1.4%, 99.8±0.8% and 100.2±1.1% for chlorprothixene, tiotixene and metixene, respectively. This procedure is applied to determine these drugs in certain formulations and the results compare favourably to compendial methods.

Key words

Analysis, quantitative Chlorprothixene Colorimetry Metixene Tiotixene 


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  1. 1.
    Foye WO. Principles of Medicinal Chemistry. Philadelphia: Lea & Febiger, 1976:218.Google Scholar
  2. 2.
    Clarke ECG. Isolation and Identification of Drugs. Part I. London: The Pharmaceutical Press, 1978:258, 414, 574.Google Scholar
  3. 3.
    Jose D. Qualitative and quantitative analysis of amitriptyline and navan. Rev Argent Farm 1972:114(3–4):42–5.Google Scholar
  4. 4.
    The United States Pharmacopeia xx, National Formulary xv. USP Convention, Inc., 1980:144,796.Google Scholar
  5. 5.
    Fedir J, Jiri K. Determination ofcis-2-chloro-9-(3-dimethylaminopropylidene)thioxanthene (chlorprothixene). Czech. 184,149 (Cl. GOIN 27/480) 15 Jul. 1980, Appl. 76/1, 661, 15 Mar. 1976; 2 pp. (C.A. 1981;94: 162839y).Google Scholar
  6. 6.
    Beltagy YA, Issa AS, Mahrous MS. Pharmaceutical analysis of some tranquilizers and antidepressants with iodine monochloride. Talanta 1978;25(6):349–51.CrossRefGoogle Scholar
  7. 7.
    Per Arne J, Ulf S, Gun H. Automatic potentiometric-2-phase titration in pharmaceutical analysis. Part 2. Determination of amine salts, amines and related compounds. Anal Chim Acta 1983;151:49–63.CrossRefGoogle Scholar
  8. 8.
    Adam O. Complex thiocyanate as precipitating agent in assaying organic bases, vi. Complex chromium thiocyanate in determining thioxanthene derivatives of pharmaceutical use. Acta Pol Pharm 1978;35:63–8.PubMedGoogle Scholar
  9. 9.
    Seija T. Determination of several thioxanthene derivatives as picrates. Acta Pharm Fenn 1978;87:195–204.Google Scholar
  10. 10.
    Seija T. Determination of chlorprothixene as its reineckate by atomic absorption spectrometry. Acta Pharm Fenn 1979;88:25–30.Google Scholar
  11. 11.
    Auterhoff H, Kuehn J. Analysis of tricyclic psychotropics. Arch Pharm 1973;306:241–8.Google Scholar
  12. 12.
    Beltagy YA. Citric acid, acetic anhydride reagent for determination of alkaloids and organic bases. Pharmazie, 1976;31:483–4.PubMedGoogle Scholar
  13. 13.
    El Sebai AI, Issa AS, Abdel Salam AS, Mahrous MS. The use of chloranil for spectrophotometric determination of some tranquilizers and antidepressants. Talanta 1983;30:531–3.CrossRefGoogle Scholar
  14. 14.
    Eiden LE, Ruth JA. Chemical and photo-oxidation of tiotixene (Navan): structure of tiotixene fluorophore. Experientia 1978;34:1062–3.PubMedGoogle Scholar
  15. 15.
    Tammilehto SA. Oxidation of chlorprothixene with potassium permanganate. J Pharm Pharmacol 1980;32:524.PubMedGoogle Scholar
  16. 16.
    Oelschlaeger H. Polarographic analysis of psychotropic drugs. Bioelectrochem Bioenerg 1983;10:25–36.CrossRefGoogle Scholar
  17. 17.
    Arne E, John J, Jan S. A procedure for simultaneous screening and quantitating of basic drugs in liver utilizing capillary GC and nitrogen sensitive detection. J Anal Toxicol 1983;7:24–8.PubMedGoogle Scholar
  18. 18.
    McLinden VJ, Stenhouse AM. A chromatography system for drug identification. Forensic Sci Int 1979;13:71–9.CrossRefPubMedGoogle Scholar
  19. 19.
    Narasimhachari N, Dorey RC, Landa BL, Friedel PO. Improved high-performance liquid chromatographic method for the quantitation of cis-tiotixene in plasma samples usingtrans-tiotixene as internal standard. J Chromatogr 1984;311:424–9.PubMedGoogle Scholar
  20. 20.
    Rizk M, Walash MI, Ibrahim F. Spectrophotometric determination of piperazine, piperazine. salts and diethylcarbamazine citrate with chloranilic acid. Spectroscopy Lett 1984;17:423–40.Google Scholar
  21. 21.
    Hinchen JD. Practical statistics for chemical research London: Methuen, 1969.Google Scholar
  22. 22.
    Hutzinger O. Electron acceptor complexes for chromogenic detection and mass spectrometric identification of phenol and aniline derivatives, related fungicides and metabolites. Anal Chem 1969;41:1662–5.CrossRefPubMedGoogle Scholar
  23. 23.
    Macke GF. Use of tetracyanoethylene as a thin layer chromatographic spray reagent. J Chromatogr 1968;36:537–9.CrossRefGoogle Scholar
  24. 24.
    Hutzinger O. Non-destructive detection of indoles by electron acceptor. J Chromatogr 1969;40:117–24.CrossRefGoogle Scholar
  25. 25.
    Hutzinger O, Heacock RA, MacNeil JD, Frei RW. Identification and analysis of naturally occurring indoles via electron donor-acceptor complexes. J Chromatogr 1972;68:173–82.CrossRefGoogle Scholar
  26. 26.
    Dhar DN. The chemistry of tetracyanoethylene. Chem Rev 1967;67:611–22.CrossRefGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1986

Authors and Affiliations

  • M. I. Walash
    • 1
  • M. Rizk
    • 1
  • A. El-Brashy
    • 1
  1. 1.Faculty of Pharmacy, Department of Analytical ChemistryMansoura UniversityMansouraEgypt

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