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Human Antibody Gene Libraries

  • Protocol
Antibody Engineering

Part of the book series: Springer Protocols Handbooks ((SPH))

The quality of antibodies selected from antibody gene libraries, in particular their affinity and the number of different clones per antigen, is a direct function of library diversity. Here, the optimization of every single step during library construction is of utmost importance for the quality. This chaper describes the generation of human naive or immune scFv antibody gene libraries by a two step cloning strategy. The method has already been proven for the generation of very high quality naive and immune libraries.

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Authors and Affiliations

  1. Abteilung Biotechnologie, Institut für Biochemie und Biotechnologie, Technische Universität Braunschweig, Spielmannstr. 7, Braunschweig, 38106, Germany

    Michael Hust & Stefan Dübel

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  1. Michael Hust
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  2. Stefan Dübel
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Correspondence to Michael Hust .

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Editors and Affiliations

  1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Roland Kontermann

  2. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Spielmannstraße 7, 38106, Braunschweig, Germany

    Stefan Dübel

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Hust, M., Dübel, S. (2010). Human Antibody Gene Libraries. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01144-3_5

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  • DOI: https://doi.org/10.1007/978-3-642-01144-3_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01143-6

  • Online ISBN: 978-3-642-01144-3

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