2010

Antibody Engineering

Editors:

ISBN: 978-3-642-01143-6 (Print) 978-3-642-01144-3 (Online)

Table of contents (50 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Cloning of the Antigen-binding Site from Hybridoma

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-14

      Cloning of Variable Domains from Mouse Hybridoma by PCR

    3. No Access

      Book Protocol

      Pages 15-20

      Coning Hybridoma cDNA by RACE

    4. No Access

      Book Protocol

      Pages 21-44

      Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly

  3. Generation of Antibody Repertoires

    1. Front Matter

      Pages 43-43

    2. No Access

      Book Protocol

      Pages 47-63

      Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens

    3. No Access

      Book Protocol

      Pages 65-84

      Human Antibody Gene Libraries

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      Book Protocol

      Pages 85-97

      Synthetic Antibody Libraries

    5. No Access

      Book Protocol

      Pages 99-114

      Immune Libraries from Nonhuman Primates (NHP)

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      Pages 115-123

      Generation of Rabbit Immune Libraries

  4. Selection of Antibody Fragments from Combinatorial Libraries

    1. Front Matter

      Pages 128-128

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      Pages 127-137

      Immunotube Selections

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      Pages 139-149

      Phage Display and Selection in Microtitre Plates

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      Pages 151-164

      Phage Display and Selections on Biotinylated Antigens

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      Pages 165-182

      Phage Display and Subtractive Selection on Cells

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      Pages 183-195

      Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells

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      Pages 197-206

      Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA

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      Pages 207-233

      Yeast Display and Selections

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      Book Protocol

      Pages 235-254

      The Generation of Transgenic Mice Expressing Human Antibody Repertoires

    10. No Access

      Book Protocol

      Pages 255-266

      Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay

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      Book Protocol

      Pages 267-287

      Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries

  5. Engineering and Production of Immunoglobulins

    1. Front Matter

      Pages 290-290

    2. No Access

      Book Protocol

      Pages 291-306

      Aspects of Isotype Selection

    3. No Access

      Book Protocol

      Pages 307-317

      Generation of Heavy and Light Chains (Chimeric Antibodies)

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