Abstract
Kinetic screening is of paramount importance when it is to select custom-made antibodies, tailored for their respective scientific, diagnostic, or pharmaceutical application. Here a kinetic screening protocol is described, using a Biacore A100 surface plasmon resonance biosensor instrument. The assay is based on an Fc-specific antibody capture system. Antibodies from complex mixtures, like from mouse hybridoma supernatants are captured on the sensor surface in an oriented manner. The method uses a single injection of one antigen concentration for the determination of six relevant screening parameters, which comprehensively describe the antibody’s kinetic rate profile and its valence mode. The method enables the scientist to rank and finally select rare and outstanding antibodies according to their kinetic signatures.
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© 2012 Springer Science+Business Media, LLC
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Schräml, M., Biehl, M. (2012). Kinetic Screening in the Antibody Development Process. In: Proetzel, G., Ebersbach, H. (eds) Antibody Methods and Protocols. Methods in Molecular Biology, vol 901. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-931-0_11
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DOI: https://doi.org/10.1007/978-1-61779-931-0_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-930-3
Online ISBN: 978-1-61779-931-0
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