Antibody Methods and Protocols

Editors:

ISBN: 978-1-61779-930-3 (Print) 978-1-61779-931-0 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-10

    Antigen Presentation for the Generation of Binding Molecules

  3. Book Protocol

    Pages 11-32

    Recombinant Antibodies and In Vitro Selection Technologies

  4. No Access

    Book Protocol

    Pages 33-51

    Phage Display

  5. No Access

    Book Protocol

    Pages 53-79

    Generation of Human Fab Libraries for Phage Display

  6. No Access

    Book Protocol

    Pages 81-99

    Selection of Human Fab Libraries by Phage Display

  7. No Access

    Book Protocol

    Pages 101-116

    Ribosome Display

  8. No Access

    Book Protocol

    Pages 117-135

    Hybridoma Technology for the Generation of Monoclonal Antibodies

  9. No Access

    Book Protocol

    Pages 137-148

    The Application of Transgenic Mice for Therapeutic Antibody Discovery

  10. No Access

    Book Protocol

    Pages 149-159

    Production of Human or Humanized Antibodies in Mice

  11. No Access

    Book Protocol

    Pages 161-170

    Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

  12. No Access

    Book Protocol

    Pages 171-181

    Kinetic Screening in the Antibody Development Process

  13. No Access

    Book Protocol

    Pages 183-194

    Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

  14. No Access

    Book Protocol

    Pages 195-208

    Determination of Antibody Glycosylation by Mass Spectrometry

  15. No Access

    Book Protocol

    Pages 209-232

    Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

  16. No Access

    Book Protocol

    Pages 233-246

    PEGylation of Antibody Fragments for Half-Life Extension

  17. No Access

    Book Protocol

    Pages 247-263

    Bispecific Antibody Derivatives Based on Full-Length IgG Formats

  18. No Access

    Book Protocol

    Pages 265-276

    Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

  19. No Access

    Book Protocol

    Pages 277-293

    Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

  20. No Access

    Book Protocol

    Pages 295-317

    Class-Specific Effector Functions of Therapeutic Antibodies

  21. No Access

    Book Protocol

    Pages E1-E1

    Antibody Methods and Protocols

  22. Back Matter

    Pages 319-325