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Gene Therapy of Salivary Diseases

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1537))

Abstract

For many years, our research group worked to develop gene transfer approaches for salivary gland disorders that lacked effective conventional therapy. The purpose of this chapter is to describe and update key methods used in this process. As described in our original chapter from the 2010 volume, we focus on one clinical condition, irradiation-induced salivary hypofunction, and address the choice of transgene and vector to be used, the construction of recombinant viral vectors, how vector delivery is accomplished, and methods for assessing vector function in vitro and in an appropriate animal model.

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Acknowledgment

The authors’ research is supported by the intramural research program of the National Institute of Dental and Craniofacial Research.

Author information

Authors and Affiliations

  1. Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA

    Bruce J. Baum M.P.T.B., N.I.D.C.R., N.I.H., Sandra Afione, John A. Chiorini, Ana P. Cotrim, Corinne M. Goldsmith & Changyu Zheng

  2. Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Building 10, Room 1N113, MSC-1190, Bethesda, MD, 20892-1190, USA

    Bruce J. Baum M.P.T.B., N.I.D.C.R., N.I.H.

Authors
  1. Bruce J. Baum M.P.T.B., N.I.D.C.R., N.I.H.
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  2. Sandra Afione
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  3. John A. Chiorini
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  4. Ana P. Cotrim
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  5. Corinne M. Goldsmith
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  6. Changyu Zheng
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Corresponding author

Correspondence to Bruce J. Baum M.P.T.B., N.I.D.C.R., N.I.H. .

Editor information

Editors and Affiliations

  1. University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  2. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  3. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

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Baum, B.J., Afione, S., Chiorini, J.A., Cotrim, A.P., Goldsmith, C.M., Zheng, C. (2017). Gene Therapy of Salivary Diseases. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 1537. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6685-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6685-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6683-7

  • Online ISBN: 978-1-4939-6685-1

  • eBook Packages: Springer Protocols

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