Book Volume 1537 2017

Oral Biology

Molecular Techniques and Applications

Editors:

ISBN: 978-1-4939-6683-7 (Print) 978-1-4939-6685-1 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Saliva and Other Oral Fluids

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-15

      Salivary Diagnostics Using Purified Nucleic Acids

    3. No Access

      Protocol

      Pages 17-36

      RNA Sequencing Analysis of Salivary Extracellular RNA

    4. No Access

      Protocol

      Pages 37-60

      Qualitative and Quantitative Proteome Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry

    5. No Access

      Protocol

      Pages 61-77

      Antioxidant Micronutrients and Oxidative Stress Biomarkers

    6. No Access

      Protocol

      Pages 79-105

      NMR-Based Metabolomics of Oral Biofluids

    7. No Access

      Protocol

      Pages 107-123

      Gene Therapy of Salivary Diseases

  3. Molecular Biosciences

    1. Front Matter

      Pages 125-125

    2. No Access

      Protocol

      Pages 127-138

      The Oral Microbiota in Health and Disease: An Overview of Molecular Findings

    3. No Access

      Protocol

      Pages 139-152

      Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

    4. No Access

      Protocol

      Pages 153-163

      Analysis of 16S rRNA Gene Amplicon Sequences Using the QIIME Software Package

    5. No Access

      Protocol

      Pages 165-190

      Adhesion of Yeast and Bacteria to Oral Surfaces

    6. No Access

      Protocol

      Pages 191-202

      Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)

    7. No Access

      Protocol

      Pages 203-218

      Methods to Study Antagonistic Activities Among Oral Bacteria

    8. No Access

      Protocol

      Pages 219-232

      Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones

    9. No Access

      Protocol

      Pages 233-247

      Markerless Genome Editing in Competent Streptococci

    10. No Access

      Protocol

      Pages 249-277

      Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns

    11. No Access

      Protocol

      Pages 279-298

      Generating Multiple Base-Resolution DNA Methylomes Using Reduced Representation Bisulfite Sequencing

    12. No Access

      Protocol

      Pages 299-306

      A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands

    13. No Access

      Protocol

      Pages 307-326

      Genome-Wide Analysis of Periodontal and Peri-Implant Cells and Tissues

    14. No Access

      Protocol

      Pages 327-345

      Differential Expression and Functional Analysis of High-Throughput -Omics Data Using Open Source Tools

    15. No Access

      Protocol

      Pages 347-364

      Exploring Genome-Wide Expression Profiles Using Machine Learning Techniques

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