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Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT)

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Cell Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1524))

Abstract

Mammalian somatic cell nuclear transfer (SCNT) is a technically and biologically challenging procedure inducing rapid reprogramming of the nucleus from the differentiated into the totipotent state in a few hours. This procedure was initially successfully accomplished in farm animals, then in rodents, and more recently in primates and in humans. Though ethical concerns regarding SCNT still exist, this procedure can be utilized to generate patient and disease-specific pluripotent embryonic stem cell lines, which carry a great promise in improving our understanding of major disease conditions and a hope for better therapies and regenerative medicine. In this section, we will survey the existing literature and describe how mouse SCNT is performed and the importance of donor cell treatment and cycle synchronization prior to SCNT.

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Author information

Authors and Affiliations

  1. Infertility and IVF Unit, Beilinson Women’s Hospital, Rabin Medical Center, Petach Tikva, Israel

    Yoel Shufaro

  2. The Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

    Yoel Shufaro

  3. Department of Obstetrics and Gynecology, and the Hadassah Human Embryonic Stem Cells Research Center, The Goldyne-Savad Institute of Gene Therapy, Hadassah-Hebrew University Hospital, Jerusalem, Israel

    Benjamin E. Reubinoff

Authors
  1. Yoel Shufaro
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  2. Benjamin E. Reubinoff
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Corresponding author

Correspondence to Yoel Shufaro .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

    Gaspar Banfalvi

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Shufaro, Y., Reubinoff, B.E. (2017). Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT). In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 1524. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6603-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6603-5_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6602-8

  • Online ISBN: 978-1-4939-6603-5

  • eBook Packages: Springer Protocols

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