Cell Cycle Synchronization

1. Front Matter

   Pages i-xv

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2. Cell Cycle Synchronization

   1. Front Matter

      Pages 1-1

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   2. Overview of Cell Synchronization

      • Gaspar Banfalvi

      Pages 3-27

3. Physical Fractionation

   1. Front Matter

      Pages 29-29

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   2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

      • Gaspar Banfalvi

      Pages 31-52

   3. Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Cells

      • Laura Senovilla, Yohann Demont, Juliette Humeau, Norma Bloy, Guido Kroemer

      Pages 53-64

   4. Large-Scale Mitotic Cell Synchronization

      • Kalyan Dulla, Anna Santamaria Margalef

      Pages 65-74

4. Chemical Blockade

   1. Front Matter

      Pages 75-75

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   2. Synchronization and Desynchronization of Cells by Interventions on the Spindle Assembly Checkpoint

      • Mohamed Jemaà, Gwenola Manic, Ilio Vitale

      Pages 77-95

   3. Synchronization of Mammalian Cell Cultures by Serum Deprivation

      • Thomas J. Langan, Kyla R. Rodgers, Richard C. Chou

      Pages 97-105

   4. DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry

      • Dorota Halicka, Hong Zhao, Jiangwei Li, Jorge Garcia, Monika Podhorecka, Zbigniew Darzynkiewicz

      Pages 107-119

   5. Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes

      • Takashi Iwamatsu

      Pages 121-147

   6. Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate

      • Cong-Jun Li

      Pages 149-159

   7. Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture

      • Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas, Gaspar Banfalvi

      Pages 161-176

   8. Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover During the Cell Cycle

      • Jennifer A. Fischer, Salvatore J. Caradonna

      Pages 177-188

   9. Synchronization of HeLa Cells

      • Hoi Tang Ma, Randy Y. C. Poon

      Pages 189-201

5. Synchronization of Unicellular Organisms

   1. Front Matter

      Pages 203-203

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   2. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

      • Gaspar Banfalvi

      Pages 205-213

   3. Synchronization of Yeast

      • Jessica Smith, Arkadi Manukyan, Hui Hua, Huzefa Dungrawala, Brandt L. Schneider

      Pages 215-242

   4. Synchronization of Pathogenic Protozoans

      • Staffan Svärd, Karin Troell

      Pages 243-252

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.

• Unicellular organisms
• Transfected cells
• Stem cells
• Physical fractionation
• centrifugal elutriation of apoptotic cells
• nuclei of mammalian cells
• large scale mitotic cells
• chromosome formation
• fertilization
• engraftment in transplantation

• Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

  Gaspar Banfalvi

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