Skip to main content

Abstract

Spores are generally defined as small reproductive bodies that get detached from the microbial species to produce a new offspring. In the process they do not fuse with other reproductive bodies, but get germinated sooner or later. They widely vary in origin, size, shape, pigmentation, biological functions, etc. Microbial spores have a diameter ranging between 1-50 µm. However, the largest spores are the fungal ascospores, for eg. Varicellaria microsticta, measuring 340 x 115 µm; the smallest spores are bacterial endospores measuring about 0.25 µm in diameter. Bacterial endospores remain dormant in the same place of origin and help the species to pass through an unfavourable period; such spores are termed as resting spores. Also, there are dispersal spores, those of Ascomycetes, whose explosive asci bursts with a hydrostatic pressure getting scattered even upto distance of 40 cm (Wolken et al., 2003).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams TT, Eiteman MA & Hansel BM, 2002, Solid-state fermentation of broiler litter for production of biocontrol agents, Bioresource Technology, 82, 33–41.

    Google Scholar 

  • Alves SB & Pereira RM, 1989, Production of Metarhizium anisopliae (Metsch.) Sorok and Beauveria bassiana (Bals) Vuill in plastic trays, Ecossistema, 14, 188–192

    Google Scholar 

  • Areas JA, Diaz BM, Lecuona RE, 1999, Bioinsecticidal activity of conidia and dry mycelium preparations of two isolates of Beauveria bassiana against the sugarcane borer Diatraea saccharalis. Journal of Biotechnology, 67, 151–8

    Google Scholar 

  • Barranco-Florida JE, Alatorre-Rosas R, Gutierrez-Rojas, Viniegra-Gonzalez & Saucedo-Casteneda, 2002, Criteria for the selection of strains of entomopathogenic fungi Verticillium lecanii for solidstate cultivation, Enzyme and Microbial Tehnology, 30, 910–915.

    Google Scholar 

  • Burtet MJG, Silva ME & Diehl Fleig E, 1997, Prducao de conidios e micelio seco de Beauveria bassiana (Bals) Viull. para contrôle de formigas cortreiras, Congresso Bras Entomol, 16, 101.

    Google Scholar 

  • Calderon A, Fraga M & Carreras B, 1995, Production of Beauveria baasiana by solid-state fermentation, Reviews on Protection of Vegetables, 10, 269–273.

    Google Scholar 

  • Capalbo DMF, Moraes IO & Moraes RO, 1994, Development of a bioreactor for semi-solid fermentation purposes: Bacterial insecticide fermentation. In: Development in Food engineering. Proc. Int. Congress Eng. Food 6th 1993 (pub 1994), Yano T, Natanno R, Nakamura K (eds), Blackie, Glasgow, UK.

    Google Scholar 

  • Capalbo DMF, Moraes IO, 1988, Production of protein protoxin by Bacillus thuringiensis by semi-solid fermentation, In: 12th Simposio Annual da Academia de Ciencias do Fstado de Sao Paulo, Campinas 1988, Vol. 11, pp 46–55.

    Google Scholar 

  • Capalbo DMF, Valicente FH, Moraes IO & Peliser LH, 2001, Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize, Electronic journal of Biotechnology, 4, 2, 112–115

    Google Scholar 

  • Casula G, Cutting SM, 2002, Bacillus Probiotics: Spore germination in the gastrointestinal tract, Applied and Environmental Microbiology, 68, 5, 2344–2352.

    Google Scholar 

  • Chen W, Li Y, Du G & Chen J, 2005, Application of resposnse surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation, World Journal of Microbiology & Biotechnology, 21, 593–599

    Google Scholar 

  • Creuly C, Larroche C & Gros JB, 1990, A fed-batch technique for 2-heptanone production by spores of Pénicillium roquefortii, Applied Microbiology and Biotechnology, 34, 20–25.

    Google Scholar 

  • Dahlberg KR & Van etten JL, 1982, Physiology and biochemistry of fungal sporulation, Annual Review of Phytopathology, 20, 281–301.

    Article  CAS  Google Scholar 

  • De Reu JC, Solid-substrate fermentation of soya beans to Tempeh process innovations and product characteristics, 1995, Ph.D. Thesis, Wageningen Agricultural Universtiy.

    Google Scholar 

  • Deshchamps F & Huet FC, 1984, ?-glucosidase production in agitated solid fermentation, study of its properties, Biotechnology Letters, 6, 451–456.

    Google Scholar 

  • Desfarges C, Larroche C & Gros JB, 1987, Spore production of Penicillium roquefortii by solid state fermentation: Stoichiometry, growth and sporulation behaviour, Biotechnology and Bioengineering, 29, 1050–1058.

    Google Scholar 

  • Desgranges C, Vergoignan C, Lereec A, Riba G & Durrand A, 1993, Use of solid state fermentatation to produce Beauveria bassiana for the biological control of European corn borer, Biotechnology Advances, 11(3), 577–587.

    Article  CAS  Google Scholar 

  • Devi PSV, Ravinder T & Jaidev C, 2005, Cost-effective production of Bacillus thuringiensis by solid-state fermentation, Journal of Invertebrate Pathology, 88, 163–168.

    Article  CAS  Google Scholar 

  • Dijksterhuis J & Samson RA, 2002, Food and crop spoilage on storage. In Mycota XI Agricultural Applications (Kemken, F., ed), pp 39–52, Springer-Verlag.

    Google Scholar 

  • Dorta B & Arcas J, 1998, Sporulation of Metarhizium anisopliae in solid-state fermentation with forced aeration, Enzyme and Microbial Technology, 23, 501–505.

    Article  CAS  Google Scholar 

  • Durand A, Renaud R, Maratray J, Almanza S & Pelletier A, 1994, Reactor for sterile solid-state fermentation methods, Patent WO 94/18306.

    Google Scholar 

  • Feng KC, Liu BL & Tzeng YM, 2000, Verticillium lecanii spore production in solid-state and liquid-state fermentations, Bioprocess Engineering, 23, 25–29

    Article  CAS  Google Scholar 

  • Feng KC, Liu BL & Tzeng YM, 2002, Morphological characterization and germination of aerial and submerged spores of the entompathogenic fungus Verticillium lecanii, World Journal of Microbiology & Biotechnology, 18, 217–224.

    Article  Google Scholar 

  • Ferron P, 1978, Biological control of insect pests by entomogenous fungi, Annual Review of Entomology, 23, 409–442.

    Article  Google Scholar 

  • Foda MS, El-Bendary MA & Moharam ME, 2003, Salient parameters involved in mosquitocidal toxins production from Bacillus spharicus by semi-solid substrate fermentation, Egypt Microbiology, 38, 229–246.

    Google Scholar 

  • Foda MS, Ismail IMK, Moharam ME & Sadek Kh. HA, 2002, A novel approach for production of Bacillus thuringiensis by solid-state fermentation, Egypt Microbiology, 37, 135–156.

    CAS  Google Scholar 

  • Gooday GW, 1981, Biogenesis of sporopollenin in fungal spore walls. In the Fungal spores; Morphogenetic controls, ed, Turian G & Hohl, HK, New York: Academic Press, pp 487–505, ISBN 0-12-703680-0.

    Google Scholar 

  • Grajek W, 1994, Sporogenesis of the entomopathogenic fungus Verticillium lecanii in solid-state cultures, Folia Microbiologica, 39, 29–32.

    Article  Google Scholar 

  • Holker U, 2000, Bioreactor for fermenting solids, Patent PCT WO 01/19954.

    Google Scholar 

  • Hussley NW & Tinsley, 1981, Impression of insect pathology in the People’s Republic of China. In: Surges, HD (Ed.), Microbial control of Pests and Plant Diseases 1970-1980. Academic Press, New York, pp 785–796.

    Google Scholar 

  • Lareo C, Sposito AF & Bossio AL, 2006 Volpe DC, Characterization of growth and sporulation of Mucor bacilliformis in solid state fermentation on an inert support, Enzyme and Microbial Technology, 38, 391–399.

    Google Scholar 

  • Larroche C & Gros JB, 1989, Strategies for spore production by Penicillium roquefortii using solid state fermentation techniques, Process Biochemistry, 24(2), 97–103.

    Google Scholar 

  • Larroche C & Gros JB, 1992, Characterization of the growth and sporulation behaviour of Penicillium roquefortii in solid substrate fermentation by material and bioenergetic balances, Biotechnology and Bioengineering, 39, 815–827.

    Article  CAS  Google Scholar 

  • Larroche C & Gros JB, 1997, Special transformation processes using fungal spores and immobilized cells, Advances in Biochemical Engineering and Biotechnology, 55, 179–220.

    CAS  Google Scholar 

  • Larroche C, Desfarges C & Gros JB, 1986, Spore production of Penicillium roquefortii in simulated solid state fermentation, Biotechnology Letters, 8(6), 453–456

    Article  CAS  Google Scholar 

  • Larroche C, Desfarges C & Gros JB, 1988, Optimization of the spore production of Pénicillium roquefortii in solid substrate fermentation on buckwheat seeds, Applied Microbiology Biotechnology, 28, 85–92

    CAS  Google Scholar 

  • Leuenberger HGW, 1984, Methodology. In; Biotechnology, Vol. 6a, Kieslick (eds), VErlag Chemie, Weinheim, Deerfield Beach, Basel, pp 5–9.

    Google Scholar 

  • Lonsane BK, Ghildayal NP, Budiatman S & Ramakrishna SV, 1985, Engineering aspects of solid state fermentation, Enzyme and Microbial Technology, 7, 258–265

    Article  CAS  Google Scholar 

  • McCoy CW, Stamper DH & Tuveson RW, 1984, Conidiogenous cell difference among mutant and wild type pathotypes of Hirsutella thompsonii var. thompsonii, Journal of Invertebrate Pathology, 43, 414–421.

    Article  Google Scholar 

  • McQuiken MP & Whipps JM, 1995, Production, survival and evaluation of solid-substrate inocula of Coniothyrium minitans against Sclerotinia sclerotiorum, European Journal of Plant Pathology, 101, 101–110

    Article  Google Scholar 

  • McQuilken MP, Budge SP & Whipps JM, 1997, Production, survival and evaluation of liquid culture produced inocula of Coniothyrium minitans against Sclerotinina sclerotiorum, Biocontrol Science and Technology, 7, 23–26.

    Article  Google Scholar 

  • Minton NP, Mauchline ML, Lemmon MJ, Brehm JK, Fox M, Michael NP, Giaccia A & Brown JM, 1995, Chemotherapeutic tumor targeting using clostridial spores, FEMS Microbial Reviews, 17, 357–364.

    Article  CAS  Google Scholar 

  • Mitchell DA, von Meien OF, Luz Jr LFL & Krieger N, 2002, Evaluation of productivity of zymotis solid-state fermentation bioreactor based on total reactor volume, Food Technology & Biotechnology, 40(2), 135–144.

    CAS  Google Scholar 

  • Moksia, J, Larroche, C & Gros JB, 1996, Gluconate production by spores of Aspergillus niger. Biotechnology Letters 18, 1025–1030.

    Article  CAS  Google Scholar 

  • Moo-Young M, Moreira AR & Tengerdy RP, 1983, Principles of solid-substrate fermentation, In; JE Smith, DR Berry, B Kristiansen (ed), The filamentous fungi, vol 4 (Fungal Technology), Edward Arnold, London, pp 117–144.

    Google Scholar 

  • Mose JR, 1970, The use of spores of bacteria for a serological tumor diagnosis (I), Z Krebsforsch, 72, 329–341.

    Google Scholar 

  • Moskowitz GJ, 1979, Inocula for blue-veined chesses and blue cheese flavour, pp 201–210, In: Peppier AJ, Perlman D (eds), Microbial Technology, Vol. 2, 2nd edition, Academic, New York & London.

    Google Scholar 

  • Munoz GA, Agosin E, Cotoras M, San Martin R & Volpe D, 1995, Comparison of aerial and submerged spore properties for Trichoderma harzianum, FEMS Microbiology Letters, 125, 63–70.

    Article  CAS  Google Scholar 

  • Oostra J, Tramper J & Rinzema A, 2000, Model-based bioreactor selection for large-scale solidstate cultivation of Coniothyrium minitans spores on oats, Enzyme and Microbial Technology, 27, 652–663.

    Article  CAS  Google Scholar 

  • Pandey A, 1992. Recent developments in solid-state fermentation, Process Biochemistry, 27, 109–117.

    Google Scholar 

  • Pandey A, 1994, Solid-state fermentation: An overview. In: Pandey, A. (Ed.), Solid-State Fermentation, Wiley Eastern, New Delhi, pp. 3–10.

    Google Scholar 

  • Pandey A, 2003, Solid-state fermentation, Biochemical Engineering Journal, 13(2-3), 81–84.

    Article  CAS  Google Scholar 

  • Pandey A, Selvakumar P, Ashakumary L & Damodaran AD, 1996, A novel process for the production of fungal spores in solid-state fermentation.. Indian patent IPA no 379/ DEL/96, Dated 23.2.96.

    Google Scholar 

  • Pandey A, Soccol CR & Mitchell D, 2000, New developments in solid-state fermentation: I-bioprocesses and products, Process Biochemistry, 35(10), 1153–1169.

    Article  CAS  Google Scholar 

  • Pascual S, De Cal A, Magan N & Melgarejo P, 2000, Surface hydrophobicity, viability and efficacy in biological control of Penicillium oxalicum spores produced in aerial and submerged culture, Journal of Applied Microbiology, 89, 847–853.

    Article  CAS  Google Scholar 

  • Raimbault M, 1981, Solid-state fermentation: growth of filamentous fungi in starchy substrate. ORSTOM 127.

    Google Scholar 

  • Ramachandran S, Fontanille P, Pandey A & Larroche C, 2007a, Spores of Aspergillus niger as reservoir of glucose oxidase synthesized during solid-state fermentation and their use as catalyst in gluconic acid production, Letters in Applied Microbiology, 44, 155–160.

    Article  CAS  Google Scholar 

  • Ramachandran S, Fontanille P, Pandey A & Larroche C, 2007b, Stability of glucose oxidase activity of A. niger spores produced by solid-state fermentation and its role as biocatalyst in bioconversion reaction, Food Technology and Biotechnology.

    Google Scholar 

  • Rotamn B, 2001, Using living spores for real-time biosensing, Genetic Engineering News, 21, 21.

    Google Scholar 

  • Roussos S, Olmas A, Raimbault M, Saucedo-Castaneda G & Lonsane BK, 1991, Startegies for large scale inoculum development for solid state fermentation system: Conidiospores of Trichoderma harzianum, Biotechnology Techniques, 5(6), 415–420.

    Article  Google Scholar 

  • Roussos S, Raimbault M, Prebois JP & Lonsane BK, 1993, Zymotis, a large scale solid state fermenter: design and evaluation, Applied Biochemistry and Biotechnology, 42, 37–52.

    Article  CAS  Google Scholar 

  • Santa HSD, Santa ORD, Brand D, Vandenberghe LPS & Soccol CR, 2005, Spore production of Beauveria bassiana from agro-industrial residues, Brazilian Archives of Biology and Technology, 48, 51–60.

    Article  Google Scholar 

  • Setlow P, 2001, Resistance of bacterial spores to ultraviolet light, Environmental Molecular Mutagenesis, 38, 97–104.

    Article  CAS  Google Scholar 

  • Silman RW, 1980, Enzyme formation during solid-substrate fermentation in rotating vessels, Biotechnology and Bioengineering, 22, 411–420.

    Article  CAS  Google Scholar 

  • Silman RW, Bothast RJ & Schisler DA, 1993, Production of Colletotrichumtruncatum for use as a mycoherbicide: Effects of culture, drying and storage on recovery and efficacy, 11, 561–575.

    CAS  Google Scholar 

  • Soccol CR, Ayala L, Soccol VT, Krueger N & Santos HR, 1997, Spore production by entomopathogenic fungus Beauveria bassiana from declassified potato by solid-state fermentation, Reviews in Microbiology, 28, 34–42.

    Google Scholar 

  • Sphicer G, Peters J, Nürnberg M & Schwebke I, 2002, Microbicidal efficacy of superheated steam II, Studies involving E. faecium and spores of B. xerothermodurans and B. coagulans, International Journal of Hygiene and Environmental Health, 204, 309–316.

    Article  Google Scholar 

  • Tarocco F, Lecuona RE, Couto AS & Areas JA, 2005, Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation using response surface methodology, Applied Microbiology and Biotechnology, 68, 481–488.

    Article  CAS  Google Scholar 

  • Viccim G, Mannich M, Capalbo DFM, Valdebenito-Sanhueza RA & Mitchell DA, 2006 Spore production in solid-state fermentation of rice by Clonostachys rosea, a biopesticide for gray mold of strawberries, Process Biochemistry.

    Google Scholar 

  • Vilas Boas AM, Andrade RM & Oliveira JV, 1996, Diversificacao de meios de cultura para producao de fungos entomotogenicos, Archives of Biology and Technology, 39, 123–128.

    Google Scholar 

  • Wang T, 1988, A simple and economic semi-solid fermentation process for Bacillus thuringiensis, International symposium on Insecticide of Bacillus thuringiensis. Hubei Academy of Agricultural Science, Wuhan, October, 1988.

    Google Scholar 

  • Weber FJ, Tramper J & Rinzema A, 1999, A simplified material and energy balance approach for process development and scale-up of Coniothyrium minitans conidia production by solid-state fermentation cultivation in a packed bed reactor, Biotechnology and Bioengineering, 65(4), 447–458.

    Article  CAS  Google Scholar 

  • Wolken WAM, Tramper J & van der Derf MJ, 2003, What spores can do for us, Trends in Biotechnology, 21(8), 338–345.

    Article  CAS  Google Scholar 

  • Wolken WAM, Tramper J & van de Werf MJ, 2002, Toxicity of terpenes to spores and mycelium of Penicillium digitatum, Biotechnology and Bioengineering, 80, 685–690.

    Article  CAS  Google Scholar 

  • Wolken WAM & van der Werf, 2001, Geraniol biotransformation-pathway in spores of Penicillium digitatum, Applied Microbiology and Biotechnology, 57, 731–737.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer Science+Business Media, LLC

About this chapter

Cite this chapter

Ramachandran, S., Larroche, C., Pandey, A. (2008). Production of Spores. In: Pandey, A., Soccol, C.R., Larroche, C. (eds) Current Developments in Solid-state Fermentation. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75213-6_11

Download citation

Publish with us

Policies and ethics