Overview
- Editors:
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James B. Pawley
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Department of Zoology, University of Wisconsin, Madison, USA
Includes detailed descriptions and in-depth comparisons of parts of the microscope itself
Covers dyes and techniques of 3D specimen prep, as well as digital data acquisition
Addresses the fundamental limitations and practical complexities of quantitative confocal fluorescence imaging
This is the revised and expanded Third Edition of a classic text
Includes supplementary material: sn.pub/extras
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About this book
Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
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Article
Open access
28 October 2023
Table of contents (52 chapters)
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- Ping-Chin Cheng, C. K. Sun
Pages 703-721
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- Ayumu Tashiro, Gloster Aaron, Dmitriy Aronov, Rosa Cossart, Daniella Dumitriu, Vivian Fenstermaker et al.
Pages 722-735
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- Mark B. Cannell, Stephen H. Cody
Pages 736-745
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- Nuno Moreno, Susan Bougourd, Jim Haseloff, José A. Feijó
Pages 769-787
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- Irina Majoul, Yiwei Jia, Rainer Duden
Pages 788-808
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- Maria A. DeBernardi, Stephen M. Hewitt, Andres Kriete
Pages 809-817
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- Ting Zhao, Robert F. Murphy
Pages 818-828
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- Paul Sims, Ralph Albrecht, James B. Pawley, Victoria Centonze, Thomas Deerinck, Jeff Hardin
Pages 846-860
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- Steffen Lindek, Nicholas J. Salmon, Ernst H. K. Stelzer
Pages 861-869
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- R. J. Palmer Jr., Janus A. J. Haagensen, Thomas R. Neu, Claus Sternberg
Pages 870-888
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Back Matter
Pages 933-985
Editors and Affiliations
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Department of Zoology, University of Wisconsin, Madison, USA
James B. Pawley